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Rizzo S, Petrella F, Zucca I, et al. In vitro labelling and detection of mesenchymal stromal cells: a comparison between magnetic resonance imaging of iron-labelled cells and magnetic resonance spectroscopy of fluorine-labelled cells. Eur Radiol Exp. 2017;1(1):6doi: 10.1186/s41747-017-0010-9.
Rizzo, S., Petrella, F., Zucca, I., Rinaldi, E., Barbaglia, A., Padelli, F., Baggi, F., Spaggiari, L., Bellomi, M., & Bruzzone, M. G. (2017). In vitro labelling and detection of mesenchymal stromal cells: a comparison between magnetic resonance imaging of iron-labelled cells and magnetic resonance spectroscopy of fluorine-labelled cells. European radiology experimental, 1(1), 6. https://doi.org/10.1186/s41747-017-0010-9
Rizzo, Stefania, et al. "In vitro labelling and detection of mesenchymal stromal cells: a comparison between magnetic resonance imaging of iron-labelled cells and magnetic resonance spectroscopy of fluorine-labelled cells." European radiology experimental vol. 1,1 (2017): 6. doi: https://doi.org/10.1186/s41747-017-0010-9
Rizzo S, Petrella F, Zucca I, Rinaldi E, Barbaglia A, Padelli F, Baggi F, Spaggiari L, Bellomi M, Bruzzone MG. In vitro labelling and detection of mesenchymal stromal cells: a comparison between magnetic resonance imaging of iron-labelled cells and magnetic resonance spectroscopy of fluorine-labelled cells. Eur Radiol Exp. 2017;1(1):6. doi: 10.1186/s41747-017-0010-9. Epub 2017 Jun 29. PMID: 29708157; PMCID: PMC5909334.
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Eur Radiol Exp. 2017;1(1):6. doi: 10.1186/s41747-017-0010-9. Epub 2017 Jun 29.

In vitro labelling and detection of mesenchymal stromal cells: a comparison between magnetic resonance imaging of iron-labelled cells and magnetic resonance spectroscopy of fluorine-labelled cells.

European radiology experimental

Stefania Rizzo, Francesco Petrella, Ileana Zucca, Elena Rinaldi, Andrea Barbaglia, Francesco Padelli, Fulvio Baggi, Lorenzo Spaggiari, Massimo Bellomi, Maria Grazia Bruzzone

Affiliations

  1. 1Department of Radiology, European Institute of Oncology, via Ripamonti 435, 20141 Milan, Italy.
  2. 2Department of Thoracic Surgery, European Institute of Oncology, Milan, Italy.
  3. 5Department of Oncology and Hemato-Oncology, Università degli Studi di Milano, via Festa del Perdono 7, 20142 Milan, Italy.
  4. Scientific Department, Neurological Institute IRCCS "Carlo Besta", Milan, Italy.
  5. Neuroimmunology and Neuromuscular Diseases Unit, Neurological Institute IRCCS "Carlo Besta", Milan, Italy.
  6. Department of Neuroradiology, Neurological Institute IRCCS "Carlo Besta", Milan, Italy.

PMID: 29708157 PMCID: PMC5909334 DOI: 10.1186/s41747-017-0010-9

Abstract

BACKGROUND: Among the various stem cell populations used for cell therapy, adult mesenchymal stromal cells (MSCs) have emerged as a major new cell technology. These cells must be tracked after transplantation to monitor their migration within the body and quantify their accumulation at the target site. This study assessed whether rat bone marrow MSCs can be labelled with superparamagnetic iron oxide (SPIO) nanoparticles and perfluorocarbon (PFC) nanoemulsion formulations without altering cell viability and compared magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) results from iron-labelled and fluorine-labelled MSCs, respectively.

METHODS: Of MSCs, 2 × 10

RESULTS: In total, 86.8% and 83.6% of MIRB-labelled cells and Cell Sense-labelled cells were viable, respectively. MIRB-labelled cells were visible in all samples with different cell numbers; pellets containing 0.5 × 10

CONCLUSIONS: Our data support the use of both types of contrast material (SPIO and PFC) for MSCs labelling, although further efforts should be dedicated to improve the efficiency of PFC labelling.

Keywords: Cell labelling; Cell tracking; Mesenchymal stromal cells (MSCs); Perfluorocarbon (PFC); Superparamagnetic iron oxide (SPIO)

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