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Front Microbiol. 2018 Apr 24;9:755. doi: 10.3389/fmicb.2018.00755. eCollection 2018.

The Membrane-Bound C Subunit of Reductive Dehalogenases: Topology Analysis and Reconstitution of the FMN-Binding Domain of PceC.

Frontiers in microbiology

GĂ©raldine F Buttet, Mathilde S Willemin, Romain Hamelin, Aamani Rupakula, Julien Maillard

Affiliations

  1. Laboratory for Environmental Biotechnology, Institute for Environmental Engineering, Swiss Federal Institute of Technology in Lausanne, Lausanne, Switzerland.
  2. Protein Core Facility, Faculty of Life Sciences, Swiss Federal Institute of Technology in Lausanne, Lausanne, Switzerland.

PMID: 29740408 PMCID: PMC5928378 DOI: 10.3389/fmicb.2018.00755

Abstract

Organohalide respiration (OHR) is the energy metabolism of anaerobic bacteria able to use halogenated organic compounds as terminal electron acceptors. While the terminal enzymes in OHR, so-called reductive dehalogenases, are well-characterized, the identity of proteins potentially involved in electron transfer to the terminal enzymes remains elusive. Among the accessory genes identified in OHR gene clusters, the C subunit (

Keywords: PceC; RdhC; flavin mononucleotide (FMN); flavin transferase; flavin-trafficking proteins (Ftp); flavoproteins; organohalide respiration; protein reconstitution

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