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PeerJ. 2018 May 04;6:e4784. doi: 10.7717/peerj.4784. eCollection 2018.

The ΦBT1 large serine recombinase catalyzes DNA integration at pseudo-.

PeerJ

Marion Herisse, Jessica L Porter, Romain Guerillot, Takehiro Tomita, Anders Goncalves Da Silva, Torsten Seemann, Benjamin P Howden, Timothy P Stinear, Sacha J Pidot

Affiliations

  1. Department of Microbiology and Immunology at the Doherty Institute, University of Melbourne, Melbourne, VIC, Australia.
  2. Microbiological Diagnostic Unit, University of Melbourne, Melbourne, VIC, Australia.
  3. Doherty Applied Microbial Genomics, University of Melbourne, Melbourne, VIC, Australia.

PMID: 29740520 PMCID: PMC5937489 DOI: 10.7717/peerj.4784

Abstract

Plasmid vectors based on bacteriophage integrases are important tools in molecular microbiology for the introduction of foreign DNA, especially into bacterial species where other systems for genetic manipulation are limited. Site specific integrases catalyze recombination between phage and bacterial attachment sites (

Keywords: DNA integration; Nocardia; Serine recombinase; Streptomyces; ΦBT1

Conflict of interest statement

Timothy P Stinear is an Academic Editor for PeerJ.

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