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García-Sarrió MJ, Sanz ML, Sanz J, et al. A new method for microwave assisted ethanolic extraction of Mentha rotundifolia bioactive terpenoids. Electrophoresis. 2018;doi: 10.1002/elps.201800115.
García-Sarrió, M. J., Sanz, M. L., Sanz, J., González-Coloma, A., & Cristina Soria, A. (2018). A new method for microwave assisted ethanolic extraction of Mentha rotundifolia bioactive terpenoids. Electrophoresis, . https://doi.org/10.1002/elps.201800115
García-Sarrió, María Jesús, et al. "A new method for microwave assisted ethanolic extraction of Mentha rotundifolia bioactive terpenoids." Electrophoresis vol. (2018). doi: https://doi.org/10.1002/elps.201800115
García-Sarrió MJ, Sanz ML, Sanz J, González-Coloma A, Cristina Soria A. A new method for microwave assisted ethanolic extraction of Mentha rotundifolia bioactive terpenoids. Electrophoresis. 2018 Apr 14; doi: 10.1002/elps.201800115. Epub 2018 Apr 14. PMID: 29656406.
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Electrophoresis. 2018 Apr 14; doi: 10.1002/elps.201800115. Epub 2018 Apr 14.

A new method for microwave assisted ethanolic extraction of Mentha rotundifolia bioactive terpenoids.

Electrophoresis

María Jesús García-Sarrió, María Luz Sanz, Jesús Sanz, Azucena González-Coloma, Ana Cristina Soria

Affiliations

  1. Dpto. Análisis Instrumental y Química Ambiental, Instituto de Química Orgánica General (CSIC), Madrid, Spain.
  2. Dpto. de Protección Vegetal, Instituto de Ciencias Agrarias (CSIC), Madrid, Spain.

PMID: 29656406 DOI: 10.1002/elps.201800115

Abstract

A new microwave-assisted extraction (MAE) method using ethanol as solvent has been optimized by means of a Box-Behnken experimental design for the enhanced extraction of bioactive terpenoids from Mentha rotundifolia leaves; 100°C, 5 min, 1.125 g dry sample: 10 mL solvent and a single extraction cycle were selected as optimal conditions. Improved performance of MAE method in terms of extraction yield and/or reproducibility over conventional solid-liquid extraction and ultrasound assisted extraction was also previously assessed. A comprehensive characterization of MAE extracts was carried out by GC-MS. A total of 46 compounds, mostly terpenoids, were identified; piperitenone oxide and piperitenone were the major compounds determined. Several neophytadiene isomers were also detected for the first time in MAE extracts. Different procedures (solid-phase extraction and activated charcoal (AC) treatment) were also evaluated for clean-up of MAE extracts, with AC providing the highest enrichment in bioactive terpenoids. Finally, the MAE method here developed is shown as a green, fast, efficient and reproducible liquid extraction methodology to obtain M. rotundifolia bioactive extracts for further application, among others, as food preservatives.

© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords: Gas chromatography-mass spectrometry (GC-MS); Mentha rotundifolia; Microwave assisted extraction (MAE); Terpenoids

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