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Lackmann JW, Wende K, Verlackt C, et al. Chemical fingerprints of cold physical plasmas - an experimental and computational study using cysteine as tracer compound. Sci Rep. 2018;8(1):7736doi: 10.1038/s41598-018-25937-0.
Lackmann, J. W., Wende, K., Verlackt, C., Golda, J., Volzke, J., Kogelheide, F., Held, J., Bekeschus, S., Bogaerts, A., Schulz-von der Gathen, V., & Stapelmann, K. (2018). Chemical fingerprints of cold physical plasmas - an experimental and computational study using cysteine as tracer compound. Scientific reports, 8(1), 7736. https://doi.org/10.1038/s41598-018-25937-0
Lackmann, J-W, et al. "Chemical fingerprints of cold physical plasmas - an experimental and computational study using cysteine as tracer compound." Scientific reports vol. 8,1 (2018): 7736. doi: https://doi.org/10.1038/s41598-018-25937-0
Lackmann JW, Wende K, Verlackt C, Golda J, Volzke J, Kogelheide F, Held J, Bekeschus S, Bogaerts A, Schulz-von der Gathen V, Stapelmann K. Chemical fingerprints of cold physical plasmas - an experimental and computational study using cysteine as tracer compound. Sci Rep. 2018 May 16;8(1):7736. doi: 10.1038/s41598-018-25937-0. PMID: 29769633; PMCID: PMC5955931.
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Sci Rep. 2018 May 16;8(1):7736. doi: 10.1038/s41598-018-25937-0.

Chemical fingerprints of cold physical plasmas - an experimental and computational study using cysteine as tracer compound.

Scientific reports

J-W Lackmann, K Wende, C Verlackt, J Golda, J Volzke, F Kogelheide, J Held, S Bekeschus, A Bogaerts, V Schulz-von der Gathen, K Stapelmann

Affiliations

  1. Biomedical Applications of Plasma Technology, Ruhr University Bochum, Universitätsstr 150, 44780, Bochum, Germany. [email protected].
  2. ZIK plasmatis, Leibniz-Institute for Plasma Science and Technology, Felix-Hausdorff-Str. 2, 17489, Greifswald, Germany. [email protected].
  3. ZIK plasmatis, Leibniz-Institute for Plasma Science and Technology, Felix-Hausdorff-Str. 2, 17489, Greifswald, Germany. [email protected].
  4. PLASMANT, University of Antwerp, Universiteitsplein 1, 2610, Antwerp-Wilrijk, Belgium.
  5. Experimental Physics II, Ruhr University Bochum, Universitätsstr 150, 44780, Bochum, Germany.
  6. ZIK plasmatis, Leibniz-Institute for Plasma Science and Technology, Felix-Hausdorff-Str. 2, 17489, Greifswald, Germany.
  7. Biomedical Applications of Plasma Technology, Ruhr University Bochum, Universitätsstr 150, 44780, Bochum, Germany.
  8. Department of Nuclear Engineering, Plasma for Life Sciences, North Carolina State University, Raleigh, NC, 27695, USA.

PMID: 29769633 PMCID: PMC5955931 DOI: 10.1038/s41598-018-25937-0

Abstract

Reactive oxygen and nitrogen species released by cold physical plasma are being proposed as effectors in various clinical conditions connected to inflammatory processes. As these plasmas can be tailored in a wide range, models to compare and control their biochemical footprint are desired to infer on the molecular mechanisms underlying the observed effects and to enable the discrimination between different plasma sources. Here, an improved model to trace short-lived reactive species is presented. Using FTIR, high-resolution mass spectrometry, and molecular dynamics computational simulation, covalent modifications of cysteine treated with different plasmas were deciphered and the respective product pattern used to generate a fingerprint of each plasma source. Such, our experimental model allows a fast and reliable grading of the chemical potential of plasmas used for medical purposes. Major reaction products were identified to be cysteine sulfonic acid, cystine, and cysteine fragments. Less-abundant products, such as oxidized cystine derivatives or S-nitrosylated cysteines, were unique to different plasma sources or operating conditions. The data collected point at hydroxyl radicals, atomic O, and singlet oxygen as major contributing species that enable an impact on cellular thiol groups when applying cold plasma in vitro or in vivo.

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