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Wagoner MD, Bohrer LR, Aldrich BT, et al. Feeder-free differentiation of cells exhibiting characteristics of corneal endothelium from human induced pluripotent stem cells. Biol Open. 2018;7(5)doi: 10.1242/bio.032102.
Wagoner, M. D., Bohrer, L. R., Aldrich, B. T., Greiner, M. A., Mullins, R. F., Worthington, K. S., Tucker, B. A., & Wiley, L. A. (2018). Feeder-free differentiation of cells exhibiting characteristics of corneal endothelium from human induced pluripotent stem cells. Biology open, 7(5), . https://doi.org/10.1242/bio.032102
Wagoner, Michael D, et al. "Feeder-free differentiation of cells exhibiting characteristics of corneal endothelium from human induced pluripotent stem cells." Biology open vol. 7,5 (2018). doi: https://doi.org/10.1242/bio.032102
Wagoner MD, Bohrer LR, Aldrich BT, Greiner MA, Mullins RF, Worthington KS, Tucker BA, Wiley LA. Feeder-free differentiation of cells exhibiting characteristics of corneal endothelium from human induced pluripotent stem cells. Biol Open. 2018 May 08;7(5). doi: 10.1242/bio.032102. PMID: 29685994; PMCID: PMC5992532.
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Biol Open. 2018 May 08;7(5). doi: 10.1242/bio.032102.

Feeder-free differentiation of cells exhibiting characteristics of corneal endothelium from human induced pluripotent stem cells.

Biology open

Michael D Wagoner, Laura R Bohrer, Benjamin T Aldrich, Mark A Greiner, Robert F Mullins, Kristan S Worthington, Budd A Tucker, Luke A Wiley

Affiliations

  1. Cornea Research Unit, Department of Ophthalmology & Visual Sciences, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.
  2. Institute for Vision Research, Department of Ophthalmology & Visual Sciences, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.
  3. Department of Ophthalmology & Visual Sciences, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.
  4. Iowa Lions Eye Bank, Coralville, IA 52241, USA.
  5. Department of Biomedical Engineering, University of Iowa, Iowa City, IA 52242, USA.
  6. Cornea Research Unit, Department of Ophthalmology & Visual Sciences, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA [email protected].

PMID: 29685994 PMCID: PMC5992532 DOI: 10.1242/bio.032102

Abstract

The purpose of this study was to devise a strategy for the derivation of corneal endothelial cells (CEnCs) from adult fibroblast-derived induced pluripotent stem cells (iPSCs). IPSCs were generated from an adult human with normal ocular history via expression of

© 2018. Published by The Company of Biologists Ltd.

Keywords: Corneal endothelial cells; Differentiation; Induced pluripotent stem cells; Neural crest cells

Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

References

  1. Ophthalmology. 2007 Dec;114(12):2281-6 - PubMed
  2. Bioessays. 2004 Apr;26(4):374-86 - PubMed
  3. Sci Rep. 2015 Nov 13;5:16595 - PubMed
  4. Ophthalmology. 2009 Jun;116(6):1023-8 - PubMed
  5. Invest Ophthalmol Vis Sci. 2015 Apr;56(4):2749-54 - PubMed
  6. PLoS One. 2015 Dec 21;10(12):e0145266 - PubMed
  7. Invest Ophthalmol Vis Sci. 2015 May;56(5):2803-10 - PubMed
  8. Ophthalmology. 2007 Sep;114(9):1630-6 - PubMed
  9. J Stem Cell Res Ther. 2014 Jan 25;3(5):161 - PubMed
  10. Sci Rep. 2017 Dec;7(1):142 - PubMed
  11. Ophthalmology. 1991 Mar;98(3):322-8 - PubMed
  12. Ophthalmology. 2015 Dec;122(12):2432-42 - PubMed
  13. Invest Ophthalmol Vis Sci. 2011 Dec 02;52(13):9291-7 - PubMed
  14. Nat Med. 2004 Jan;10(1):55-63 - PubMed
  15. Invest Ophthalmol Vis Sci. 2016 Feb;57(2):771-2 - PubMed
  16. Sci Rep. 2017 Jun 22;7(1):4074 - PubMed
  17. Eye (Lond). 2007 Oct;21(10):1310-8 - PubMed
  18. Sci Rep. 2016 Jul 29;6:30742 - PubMed
  19. Ophthalmology. 2011 Apr;118(4):725-9 - PubMed
  20. Semin Ophthalmol. 2013 Sep-Nov;28(5-6):281-6 - PubMed
  21. Stem Cells Transl Med. 2017 Jun;6(6):1533-1546 - PubMed
  22. Transl Res. 2015 Dec;166(6):740-749.e1 - PubMed
  23. Graefes Arch Clin Exp Ophthalmol. 2013 Apr;251(4):1191-8 - PubMed
  24. Ophthalmology. 2016 Jun;123(6):1232-6 - PubMed
  25. Invest Ophthalmol Vis Sci. 2017 Mar 1;58(3):1368-1377 - PubMed
  26. Stem Cell Res. 2017 May;21:58-70 - PubMed
  27. Ophthalmology. 2009 Sep;116(9):1818-30 - PubMed
  28. Invest Ophthalmol Vis Sci. 2017 Feb 1;58(2):892-900 - PubMed
  29. Am J Pathol. 2010 Nov;177(5):2278-89 - PubMed
  30. Curr Protoc Stem Cell Biol. 2017 Aug 14;42:4A.12.1-4A.12.14 - PubMed
  31. Sci Rep. 2017 Jul 28;7(1):6801 - PubMed
  32. Ophthalmology. 2003 Jul;110(7):1396-402 - PubMed
  33. Int Ophthalmol. 2008 Jun;28(3):191-207 - PubMed
  34. Invest Ophthalmol Vis Sci. 2017 Apr 1;58(4):2130-2138 - PubMed
  35. Cold Spring Harb Perspect Biol. 2012 Dec 01;4(12):null - PubMed
  36. Int Ophthalmol. 2012 Feb;32(1):9-14 - PubMed
  37. Nat Protoc. 2013 Jan;8(1):203-12 - PubMed
  38. Lab Invest. 2015 Nov;95(11):1291-304 - PubMed
  39. Hum Mol Genet. 2013 Feb 1;22(3):593-607 - PubMed
  40. Invest Ophthalmol Vis Sci. 2016 Dec 1;57(15):6878-6884 - PubMed
  41. Ophthalmology. 2007 Jun;114(6):1073-9 - PubMed
  42. Cornea. 2009 May;28(4):385-94 - PubMed
  43. Curr Protoc Stem Cell Biol. 2016 May 12;37:1C.19.1-1C.19.11 - PubMed
  44. Ophthalmic Genet. 2013 Mar-Jun;34(1-2):32-4 - PubMed
  45. Cornea. 2014 Aug;33(8):785-9 - PubMed
  46. J Biol Chem. 2015 Mar 6;290(10):5979-90 - PubMed
  47. Cornea. 2007 Jul;26(6):670-4 - PubMed
  48. Nat Commun. 2014 Jun 10;5:4047 - PubMed
  49. Mol Ther. 2017 Sep 6;25(9):1999-2013 - PubMed
  50. Sci Rep. 2016 Jul 06;6:29047 - PubMed
  51. Invest Ophthalmol Vis Sci. 2016 Nov 1;57(14 ):5926-5934 - PubMed
  52. Br J Ophthalmol. 2006 Mar;90(3):324-7 - PubMed
  53. Surv Ophthalmol. 2012 May-Jun;57(3):236-52 - PubMed
  54. Invest Ophthalmol Vis Sci. 2015 Dec;56(13):8258-67 - PubMed
  55. Cornea. 2017 Jul;36(7):866-874 - PubMed
  56. Stem Cells Dev. 2014 Sep 15;23(18):2190-201 - PubMed
  57. J Tissue Eng Regen Med. 2017 Apr;11(4):1057-1064 - PubMed

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