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Mohamed Yusoff AA, Zulfakhar FN, Mohd Khair SZN, et al. Mitochondrial 10398A>G NADH-Dehydrogenase Subunit 3 of Complex I Is Frequently Altered in Intra-Axial Brain Tumors in Malaysia. Brain Tumor Res Treat. 2018;6(1):31-38doi: 10.14791/btrt.2018.6.e5.
Mohamed Yusoff, A. A., Zulfakhar, F. N., Mohd Khair, S. Z. N., Wan Abdullah, W. S., Abdullah, J. M., & Idris, Z. (2018). Mitochondrial 10398A>G NADH-Dehydrogenase Subunit 3 of Complex I Is Frequently Altered in Intra-Axial Brain Tumors in Malaysia. Brain tumor research and treatment, 6(1), 31-38. https://doi.org/10.14791/btrt.2018.6.e5
Mohamed Yusoff, Abdul Aziz, et al. "Mitochondrial 10398A>G NADH-Dehydrogenase Subunit 3 of Complex I Is Frequently Altered in Intra-Axial Brain Tumors in Malaysia." Brain tumor research and treatment vol. 6,1 (2018): 31-38. doi: https://doi.org/10.14791/btrt.2018.6.e5
Mohamed Yusoff AA, Zulfakhar FN, Mohd Khair SZN, Wan Abdullah WS, Abdullah JM, Idris Z. Mitochondrial 10398A>G NADH-Dehydrogenase Subunit 3 of Complex I Is Frequently Altered in Intra-Axial Brain Tumors in Malaysia. Brain Tumor Res Treat. 2018 Apr;6(1):31-38. doi: 10.14791/btrt.2018.6.e5. PMID: 29717568; PMCID: PMC5932297.
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Brain Tumor Res Treat. 2018 Apr;6(1):31-38. doi: 10.14791/btrt.2018.6.e5.

Mitochondrial 10398A>G NADH-Dehydrogenase Subunit 3 of Complex I Is Frequently Altered in Intra-Axial Brain Tumors in Malaysia.

Brain tumor research and treatment

Abdul Aziz Mohamed Yusoff, Fatin Najwa Zulfakhar, Siti Zulaikha Nashwa Mohd Khair, Wan Salihah Wan Abdullah, Jafri Malin Abdullah, Zamzuri Idris

Affiliations

  1. Department of Neurosciences, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kelantan, Malaysia. [email protected].
  2. School of Health Sciences, Universiti Sains Malaysia, Health Campus, Kelantan, Malaysia.
  3. Department of Neurosciences, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kelantan, Malaysia.
  4. Center for Neuroscience Services and Research, Universiti Sains Malaysia, Health Campus, Kelantan, Malaysia.

PMID: 29717568 PMCID: PMC5932297 DOI: 10.14791/btrt.2018.6.e5

Abstract

BACKGROUND: Mitochondria are major cellular sources of reactive oxygen species (ROS) generation which can induce mitochondrial DNA damage and lead to carcinogenesis. The mitochondrial 10398A>G alteration in NADH-dehydrogenase subunit 3 (ND3) can severely impair complex I, a key component of ROS production in the mitochondrial electron transport chain. Alteration in ND3 10398A>G has been reported to be linked with diverse neurodegenerative disorders and cancers. The aim of this study was to find out the association of mitochondrial ND3 10398A>G alteration in brain tumor of Malaysian patients.

METHODS: Brain tumor tissues and corresponding blood specimens were obtained from 45 patients. The ND3 10398A>G alteration at target codon 114 was detected using the PCR-RFLP analysis and later was confirmed by DNA sequencing.

RESULTS: Twenty-six (57.8%) patients showed ND3 10398A>G mutation in their tumor specimens, in which 26.9% of these mutations were heterozygous mutations. ND3 10398A>G mutation was not significantly correlated with age, gender, and histological tumor grade, however was found more frequently in intra-axial than in extra-axial tumors (62.5% vs. 46.2%, p<0.01).

CONCLUSION: For the first time, we have been able to describe the occurrence of ND3 10398A>G mutations in a Malaysian brain tumor population. It can be concluded that mitochondrial ND3 10398A>G alteration is frequently present in brain tumors among Malaysian population and it shows an impact on the intra-axial tumors.

Copyright © 2018 The Korean Brain Tumor Society, The Korean Society for Neuro-Oncology, and The Korean Society for Pediatric Neuro-Oncology.

Keywords: Brain tumor; Malaysia; Mitochondrial DNA; ND3 A10398G mutation

Conflict of interest statement

The authors have no financial conflicts of interest.

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