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Glaser AK, Reder NP, Chen Y, et al. Light-sheet microscopy for slide-free non-destructive pathology of large clinical specimens. Nat Biomed Eng. 2017;1(7)doi: 10.1038/s41551-017-0084.
Glaser, A. K., Reder, N. P., Chen, Y., McCarty, E. F., Yin, C., Wei, L., Wang, Y., True, L. D., & Liu, J. T. C. (2017). Light-sheet microscopy for slide-free non-destructive pathology of large clinical specimens. Nature biomedical engineering, 1(7), . https://doi.org/10.1038/s41551-017-0084
Glaser, Adam K, et al. "Light-sheet microscopy for slide-free non-destructive pathology of large clinical specimens." Nature biomedical engineering vol. 1,7 (2017). doi: https://doi.org/10.1038/s41551-017-0084
Glaser AK, Reder NP, Chen Y, McCarty EF, Yin C, Wei L, Wang Y, True LD, Liu JTC. Light-sheet microscopy for slide-free non-destructive pathology of large clinical specimens. Nat Biomed Eng. 2017 Jul;1(7). doi: 10.1038/s41551-017-0084. Epub 2017 Jun 26. PMID: 29750130; PMCID: PMC5940348.
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Nat Biomed Eng. 2017 Jul;1(7). doi: 10.1038/s41551-017-0084. Epub 2017 Jun 26.

Light-sheet microscopy for slide-free non-destructive pathology of large clinical specimens.

Nature biomedical engineering

Adam K Glaser, Nicholas P Reder, Ye Chen, Erin F McCarty, Chengbo Yin, Linpeng Wei, Yu Wang, Lawrence D True, Jonathan T C Liu

Affiliations

  1. Department of Mechanical Engineering, University of Washington, Seattle, WA USA.
  2. Department of Pathology, University of Washington, Seattle, WA USA.

PMID: 29750130 PMCID: PMC5940348 DOI: 10.1038/s41551-017-0084

Abstract

For the 1.7 million patients per year in the U.S. who receive a new cancer diagnosis, treatment decisions are largely made after a histopathology exam. Unfortunately, the gold standard of slide-based microscopic pathology suffers from high inter-observer variability and limited prognostic value due to sampling limitations and the inability to visualize tissue structures and molecular targets in their native 3D context. Here, we show that an open-top light-sheet microscope optimized for non-destructive slide-free pathology of clinical specimens enables the rapid imaging of intact tissues at high resolution over large 2D and 3D fields of view, with the same level of detail as traditional pathology. We demonstrate the utility of this technology for various applications: wide-area surface microscopy to triage surgical specimens (with ~200 μm surface irregularities), rapid intraoperative assessment of tumour-margin surfaces (12.5 sec/cm2), and volumetric assessment of optically cleared core-needle biopsies (1 mm in diameter, 2 cm in length). Light-sheet microscopy can be a versatile tool for both rapid surface microscopy and deep volumetric microscopy of human specimens.

Conflict of interest statement

Competing interests The authors declare no competing financial interests.

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