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Tajsoleiman T, Abdekhodaie MJ, Gernaey KV, et al. Efficient Computational Design of a Scaffold for Cartilage Cell Regeneration. Bioengineering (Basel). 2018;5(2)doi: 10.3390/bioengineering5020033.
Tajsoleiman, T., Abdekhodaie, M. J., Gernaey, K. V., & Krühne, U. (2018). Efficient Computational Design of a Scaffold for Cartilage Cell Regeneration. Bioengineering (Basel, Switzerland), 5(2), . https://doi.org/10.3390/bioengineering5020033
Tajsoleiman, Tannaz, et al. "Efficient Computational Design of a Scaffold for Cartilage Cell Regeneration." Bioengineering (Basel, Switzerland) vol. 5,2 (2018). doi: https://doi.org/10.3390/bioengineering5020033
Tajsoleiman T, Abdekhodaie MJ, Gernaey KV, Krühne U. Efficient Computational Design of a Scaffold for Cartilage Cell Regeneration. Bioengineering (Basel). 2018 Apr 24;5(2). doi: 10.3390/bioengineering5020033. PMID: 29695105; PMCID: PMC6027378.
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Bioengineering (Basel). 2018 Apr 24;5(2). doi: 10.3390/bioengineering5020033.

Efficient Computational Design of a Scaffold for Cartilage Cell Regeneration.

Bioengineering (Basel, Switzerland)

Tannaz Tajsoleiman, Mohammad Jafar Abdekhodaie, Krist V Gernaey, Ulrich Krühne

Affiliations

  1. Department of Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800 Kgs., Lyngby, Denmark. [email protected].
  2. Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran. [email protected].
  3. Department of Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800 Kgs., Lyngby, Denmark. [email protected].
  4. Department of Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800 Kgs., Lyngby, Denmark. [email protected].

PMID: 29695105 PMCID: PMC6027378 DOI: 10.3390/bioengineering5020033

Abstract

Due to the sensitivity of mammalian cell cultures, understanding the influence of operating conditions during a tissue generation procedure is crucial. In this regard, a detailed study of scaffold based cell culture under a perfusion flow is presented with the aid of mathematical modelling and computational fluid dynamics (CFD). With respect to the complexity of the case study, this work focuses solely on the effect of nutrient and metabolite concentrations, and the possible influence of fluid-induced shear stress on a targeted cell (cartilage) culture. The simulation set up gives the possibility of predicting the cell culture behavior under various operating conditions and scaffold designs. Thereby, the exploitation of the predictive simulation into a newly developed stochastic routine provides the opportunity of exploring improved scaffold geometry designs. This approach was applied on a common type of fibrous structure in order to increase the process efficiencies compared with the regular used formats. The suggested topology supplies a larger effective surface for cell attachment compared to the reference design while the level of shear stress is kept at the positive range of effect. Moreover, significant improvement of mass transfer is predicted for the suggested topology.

Keywords: CFD simulation; micro-bioreactor operating conditions; scaffold geometry optimization; tissue engineering

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