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Nielsen T, Kristensen AF, Pedersen S, et al. Investigation of procoagulant activity in extracellular vesicles isolated by differential ultracentrifugation. J Extracell Vesicles. 2018;7(1):1454777doi: 10.1080/20013078.2018.1454777.
Nielsen, T., Kristensen, A. F., Pedersen, S., Christiansen, G., & Kristensen, S. R. (2018). Investigation of procoagulant activity in extracellular vesicles isolated by differential ultracentrifugation. Journal of extracellular vesicles, 7(1), 1454777. https://doi.org/10.1080/20013078.2018.1454777
Nielsen, Thøger, et al. "Investigation of procoagulant activity in extracellular vesicles isolated by differential ultracentrifugation." Journal of extracellular vesicles vol. 7,1 (2018): 1454777. doi: https://doi.org/10.1080/20013078.2018.1454777
Nielsen T, Kristensen AF, Pedersen S, Christiansen G, Kristensen SR. Investigation of procoagulant activity in extracellular vesicles isolated by differential ultracentrifugation. J Extracell Vesicles. 2018 Mar 26;7(1):1454777. doi: 10.1080/20013078.2018.1454777. eCollection 2018. PMID: 29696077; PMCID: PMC5912197.
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J Extracell Vesicles. 2018 Mar 26;7(1):1454777. doi: 10.1080/20013078.2018.1454777. eCollection 2018.

Investigation of procoagulant activity in extracellular vesicles isolated by differential ultracentrifugation.

Journal of extracellular vesicles

Thøger Nielsen, Anne Flou Kristensen, Shona Pedersen, Gunna Christiansen, Søren Risom Kristensen

Affiliations

  1. Department of Clinical Biochemistry, Aalborg University Hospital, Aalborg, Denmark.
  2. Department of Clinical Medicine, Aalborg University, Aalborg, Denmark.
  3. Department of Biomedicine, Aarhus University, Aarhus, Denmark.

PMID: 29696077 PMCID: PMC5912197 DOI: 10.1080/20013078.2018.1454777

Abstract

Tissue factor (TF) is the main initiator of coagulation and procoagulant phospholipids (PPL) are key components in promoting coagulation activity in blood. Both TF and PPL may be presented on the surface of extracellular vesicles (EVs), thus contributing to their procoagulant activity. These EVs may constitute a substantial part of pathological hypercoagulability that is responsible for triggering a higher risk of thrombosis in certain patients. The aim of this study was to describe a model system for the isolation of EVs required for investigating their effect on coagulation. Differential ultracentrifugation (DUC) with and without a single washing step was used to isolate and evaluate the procoagulant capacity of EVs from healthy volunteers through analysis of thrombin generation and PPL activity. Ultracentrifugation at 20,000 × 

Keywords: Electron microscopy; extracellular vesicles; isolation; microvesicles; procoagulant phospholipids; thrombin generation; tissue factor

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