Display options
Cite
Tian H, Zhou X, Chen C, et al. Simultaneous Determination of Phenobarbital, Pentobarbital, Amobarbital and Secobarbital in Raw Milk via Liquid Chromatography with Electron Spray Ionization Tandem Mass Spectrometry. Korean J Food Sci Anim Resour. 2017;37(6):847-854doi: 10.5851/kosfa.2017.37.6.847.
Tian, H., Zhou, X., Chen, C., He, Y., Yu, H., & Zheng, X. (2017). Simultaneous Determination of Phenobarbital, Pentobarbital, Amobarbital and Secobarbital in Raw Milk via Liquid Chromatography with Electron Spray Ionization Tandem Mass Spectrometry. Korean journal for food science of animal resources, 37(6), 847-854. https://doi.org/10.5851/kosfa.2017.37.6.847
Tian, Huaixiang, et al. "Simultaneous Determination of Phenobarbital, Pentobarbital, Amobarbital and Secobarbital in Raw Milk via Liquid Chromatography with Electron Spray Ionization Tandem Mass Spectrometry." Korean journal for food science of animal resources vol. 37,6 (2017): 847-854. doi: https://doi.org/10.5851/kosfa.2017.37.6.847
Tian H, Zhou X, Chen C, He Y, Yu H, Zheng X. Simultaneous Determination of Phenobarbital, Pentobarbital, Amobarbital and Secobarbital in Raw Milk via Liquid Chromatography with Electron Spray Ionization Tandem Mass Spectrometry. Korean J Food Sci Anim Resour. 2017;37(6):847-854. doi: 10.5851/kosfa.2017.37.6.847. Epub 2017 Dec 31. PMID: 29725206; PMCID: PMC5932935.
Copy Download .nbib Format: NLM
Share it on

Korean J Food Sci Anim Resour. 2017;37(6):847-854. doi: 10.5851/kosfa.2017.37.6.847. Epub 2017 Dec 31.

Simultaneous Determination of Phenobarbital, Pentobarbital, Amobarbital and Secobarbital in Raw Milk via Liquid Chromatography with Electron Spray Ionization Tandem Mass Spectrometry.

Korean journal for food science of animal resources

Huaixiang Tian, Xingxin Zhou, Chen Chen, Yabin He, Haiyan Yu, Xiaoping Zheng

Affiliations

  1. School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 200436, China.
  2. Shanghai Bino Testing Technology Service, Shanghai, China.

PMID: 29725206 PMCID: PMC5932935 DOI: 10.5851/kosfa.2017.37.6.847

Abstract

A rapid, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous determination of four barbiturates (phenobarbital, pentobarbital, amobarbital and secobarbital) in raw milk. The barbiturates were extracted using liquid-liquid extraction, ultrasonication and centrifugation, and purified on an SPE column. Analytes were separated by HPLC on a CSH C18 column eluted using an acetonitrile-water system with a linear gradient dilution programme, and detected by MS/MS. The recoveries of the barbiturates were 85.0-113.5%, and the intra- and inter-assay RSDs were less than 9.8% and 7.3%, respectively. The limit of detection was 5 ng/mL for all four of the barbiturates. The analytical method exhibited good linearity from 10 to 1000 ng/mL; the correlation coefficient (

Keywords: LC-MS/MS; barbiturates; raw milk; veterinary drug residue

References

  1. Pharmazie. 2014 Jun;69(6):417-9 - PubMed
  2. Psychosomatics. 2016 Jul-Aug;57(4):341-7 - PubMed
  3. Anal Chem. 2004 Feb 1;76(3):839-47 - PubMed
  4. Anal Sci. 2016;32(6):645-52 - PubMed
  5. Forensic Sci Int. 2011 Mar 20;206(1-3):119-26 - PubMed
  6. Respir Physiol Neurobiol. 2004 Jun 25;140(3):219-30 - PubMed
  7. J Chromatogr A. 2000 Feb 18;870(1-2):39-44 - PubMed
  8. Pediatr Rev. 1997 Aug;18(8):260-4; quiz 265 - PubMed
  9. J Appl Physiol Respir Environ Exerc Physiol. 1977 Aug;43(2):221-9 - PubMed
  10. Pharm Biol. 2017 Dec;55(1):937-945 - PubMed
  11. Drug Test Anal. 2017 Apr;9(4):588-595 - PubMed
  12. J Mass Spectrom. 2004 Jan;39(1):43-50 - PubMed
  13. Biomed Chromatogr. 2010 Jan;24(1):49-65 - PubMed
  14. Food Chem. 2016 Jan 1;190:982-989 - PubMed
  15. J Pharm Biomed Anal. 2015 Oct 10;114:455-61 - PubMed
  16. Methods Mol Biol. 2010;603:65-74 - PubMed
  17. J Forensic Sci. 2012 May;57(3):813-9 - PubMed
  18. Scand J Clin Lab Invest. 1993 Nov;53(7):757-63 - PubMed
  19. J Dairy Sci. 2004 Nov;87(11):3889-98 - PubMed
  20. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jun 1;852(1-2):22-34 - PubMed
  21. Arch Int Pharmacodyn Ther. 1974 May;209(1):127-43 - PubMed

Publication Types