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Washetine K, Kara-Borni M, Heeke S, et al. Ensuring the Safety and Security of Frozen Lung Cancer Tissue Collections through the Encapsulation of Dried DNA. Cancers (Basel). 2018;10(6)doi: 10.3390/cancers10060195.
Washetine, K., Kara-Borni, M., Heeke, S., Bonnetaud, C., Félix, J. M., Ribeyre, L., Bence, C., Ilié, M., Bordone, O., Pedro, M., Maitre, P., Tanga, V., Gormally, E., Mossuz, P., Lorimier, P., Marquette, C. H., Mouroux, J., Cohen, C., Lassalle, S., Long-Mira, E., Clément, B., Dagher, G., Hofman, V., & Hofman, P. (2018). Ensuring the Safety and Security of Frozen Lung Cancer Tissue Collections through the Encapsulation of Dried DNA. Cancers, 10(6), . https://doi.org/10.3390/cancers10060195
Washetine, Kevin, et al. "Ensuring the Safety and Security of Frozen Lung Cancer Tissue Collections through the Encapsulation of Dried DNA." Cancers vol. 10,6 (2018). doi: https://doi.org/10.3390/cancers10060195
Washetine K, Kara-Borni M, Heeke S, Bonnetaud C, Félix JM, Ribeyre L, Bence C, Ilié M, Bordone O, Pedro M, Maitre P, Tanga V, Gormally E, Mossuz P, Lorimier P, Marquette CH, Mouroux J, Cohen C, Lassalle S, Long-Mira E, Clément B, Dagher G, Hofman V, Hofman P. Ensuring the Safety and Security of Frozen Lung Cancer Tissue Collections through the Encapsulation of Dried DNA. Cancers (Basel). 2018 Jun 11;10(6). doi: 10.3390/cancers10060195. PMID: 29891792; PMCID: PMC6025404.
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Cancers (Basel). 2018 Jun 11;10(6). doi: 10.3390/cancers10060195.

Ensuring the Safety and Security of Frozen Lung Cancer Tissue Collections through the Encapsulation of Dried DNA.

Cancers

Kevin Washetine, Mehdi Kara-Borni, Simon Heeke, Christelle Bonnetaud, Jean-Marc Félix, Lydia Ribeyre, Coraline Bence, Marius Ilié, Olivier Bordone, Marine Pedro, Priscilla Maitre, Virginie Tanga, Emmanuelle Gormally, Pascal Mossuz, Philippe Lorimier, Charles Hugo Marquette, Jérôme Mouroux, Charlotte Cohen, Sandra Lassalle, Elodie Long-Mira, Bruno Clément, Georges Dagher, Véronique Hofman, Paul Hofman

Affiliations

  1. Hospital-Integrated Biobank (BB-0033-00025), Université Côte d'Azur, CHU de Nice, 06001 Nice CEDEX 1, France. [email protected].
  2. Hospital-Integrated Biobank (BB-0033-00025), Université Côte d'Azur, CHU de Nice, 06001 Nice CEDEX 1, France. [email protected].
  3. Team 4, Institute of Research on Cancer and Aging of Nice (IRCAN), Inserm U1081, CNRS UMR7284, Université Côte d'Azur, CHU de Nice, 06107 Nice CEDEX 2, France. [email protected].
  4. Hospital-Integrated Biobank (BB-0033-00025), Université Côte d'Azur, CHU de Nice, 06001 Nice CEDEX 1, France. [email protected].
  5. Hospital-Integrated Biobank (BB-0033-00025), Université Côte d'Azur, CHU de Nice, 06001 Nice CEDEX 1, France. [email protected].
  6. Hospital-Integrated Biobank (BB-0033-00025), Université Côte d'Azur, CHU de Nice, 06001 Nice CEDEX 1, France. [email protected].
  7. Team 4, Institute of Research on Cancer and Aging of Nice (IRCAN), Inserm U1081, CNRS UMR7284, Université Côte d'Azur, CHU de Nice, 06107 Nice CEDEX 2, France. [email protected].
  8. Laboratory of Clinical and Experimental Pathology, Université Côte d'Azur, CHU de Nice, University Hospital Federation OncoAge, 06001 Nice CEDEX 1, France. [email protected].
  9. Hospital-Integrated Biobank (BB-0033-00025), Université Côte d'Azur, CHU de Nice, 06001 Nice CEDEX 1, France. [email protected].
  10. Team 4, Institute of Research on Cancer and Aging of Nice (IRCAN), Inserm U1081, CNRS UMR7284, Université Côte d'Azur, CHU de Nice, 06107 Nice CEDEX 2, France. [email protected].
  11. Laboratory of Clinical and Experimental Pathology, Université Côte d'Azur, CHU de Nice, University Hospital Federation OncoAge, 06001 Nice CEDEX 1, France. [email protected].
  12. FHU OncoAge, University of Nice Sophia Antipolis, 06001 Nice CEDEX 1, France. [email protected].
  13. Hospital-Integrated Biobank (BB-0033-00025), Université Côte d'Azur, CHU de Nice, 06001 Nice CEDEX 1, France. [email protected].
  14. Hospital-Integrated Biobank (BB-0033-00025), Université Côte d'Azur, CHU de Nice, 06001 Nice CEDEX 1, France. [email protected].
  15. Hospital-Integrated Biobank (BB-0033-00025), Université Côte d'Azur, CHU de Nice, 06001 Nice CEDEX 1, France. [email protected].
  16. Hospital-Integrated Biobank (BB-0033-00025), Université Côte d'Azur, CHU de Nice, 06001 Nice CEDEX 1, France. [email protected].
  17. Université de Lyon, UMRS 449; Laboratoire de Biologie générale, Université Catholique de Lyon; Reproduction et développement comparé, EPHE, 69002 Lyon, France. [email protected].
  18. Biobank of Grenoble (BB-0033-00069), 38043 Grenoble, France. [email protected].
  19. Biobank of Grenoble (BB-0033-00069), 38043 Grenoble, France. [email protected].
  20. Team 4, Institute of Research on Cancer and Aging of Nice (IRCAN), Inserm U1081, CNRS UMR7284, Université Côte d'Azur, CHU de Nice, 06107 Nice CEDEX 2, France. [email protected].
  21. FHU OncoAge, University of Nice Sophia Antipolis, 06001 Nice CEDEX 1, France. [email protected].
  22. Department of Pulmonary Medicine and Oncology, Université Côte d'Azur, CHU de Nice, University Hospital Federation OncoAge, 06001 Nice CEDEX 1, France. [email protected].
  23. Department of Thoracic Surgery, Université Côte d'Azur, CHU de Nice, University Hospital Federation OncoAge, 06001 Nice CEDEX 1, France. [email protected].
  24. Department of Thoracic Surgery, Université Côte d'Azur, CHU de Nice, University Hospital Federation OncoAge, 06001 Nice CEDEX 1, France. [email protected].
  25. Hospital-Integrated Biobank (BB-0033-00025), Université Côte d'Azur, CHU de Nice, 06001 Nice CEDEX 1, France. [email protected].
  26. Team 4, Institute of Research on Cancer and Aging of Nice (IRCAN), Inserm U1081, CNRS UMR7284, Université Côte d'Azur, CHU de Nice, 06107 Nice CEDEX 2, France. [email protected].
  27. Laboratory of Clinical and Experimental Pathology, Université Côte d'Azur, CHU de Nice, University Hospital Federation OncoAge, 06001 Nice CEDEX 1, France. [email protected].
  28. FHU OncoAge, University of Nice Sophia Antipolis, 06001 Nice CEDEX 1, France. [email protected].
  29. Hospital-Integrated Biobank (BB-0033-00025), Université Côte d'Azur, CHU de Nice, 06001 Nice CEDEX 1, France. [email protected].
  30. Team 4, Institute of Research on Cancer and Aging of Nice (IRCAN), Inserm U1081, CNRS UMR7284, Université Côte d'Azur, CHU de Nice, 06107 Nice CEDEX 2, France. [email protected].
  31. Laboratory of Clinical and Experimental Pathology, Université Côte d'Azur, CHU de Nice, University Hospital Federation OncoAge, 06001 Nice CEDEX 1, France. [email protected].
  32. FHU OncoAge, University of Nice Sophia Antipolis, 06001 Nice CEDEX 1, France. [email protected].
  33. INSERM INRA, NuMeCan, Rennes University, CRB Santé, CHU Rennes, 35000 Rennes, France. [email protected].
  34. INSERM, 75654 Paris, France. [email protected].
  35. Hospital-Integrated Biobank (BB-0033-00025), Université Côte d'Azur, CHU de Nice, 06001 Nice CEDEX 1, France. [email protected].
  36. Team 4, Institute of Research on Cancer and Aging of Nice (IRCAN), Inserm U1081, CNRS UMR7284, Université Côte d'Azur, CHU de Nice, 06107 Nice CEDEX 2, France. [email protected].
  37. Laboratory of Clinical and Experimental Pathology, Université Côte d'Azur, CHU de Nice, University Hospital Federation OncoAge, 06001 Nice CEDEX 1, France. [email protected].
  38. FHU OncoAge, University of Nice Sophia Antipolis, 06001 Nice CEDEX 1, France. [email protected].
  39. Hospital-Integrated Biobank (BB-0033-00025), Université Côte d'Azur, CHU de Nice, 06001 Nice CEDEX 1, France. [email protected].
  40. Team 4, Institute of Research on Cancer and Aging of Nice (IRCAN), Inserm U1081, CNRS UMR7284, Université Côte d'Azur, CHU de Nice, 06107 Nice CEDEX 2, France. [email protected].
  41. Laboratory of Clinical and Experimental Pathology, Université Côte d'Azur, CHU de Nice, University Hospital Federation OncoAge, 06001 Nice CEDEX 1, France. [email protected].
  42. FHU OncoAge, University of Nice Sophia Antipolis, 06001 Nice CEDEX 1, France. [email protected].

PMID: 29891792 PMCID: PMC6025404 DOI: 10.3390/cancers10060195

Abstract

Collected specimens for research purposes may or may not be made available depending on their scarcity and/or on the project needs. Their protection against degradation or in the event of an incident is pivotal. Duplication and storage on a different site is the best way to assure their sustainability. The conservation of samples at room temperature (RT) by duplication can facilitate their protection. We describe a security system for the collection of non-small cell lung cancers (NSCLC) stored in the biobank of the Nice Hospital Center, France, by duplication and conservation of lyophilized (dried), encapsulated DNA kept at RT. Therefore, three frozen tissue collections from non-smoking, early stage and sarcomatoid carcinoma NSCLC patients were selected for this study. DNA was extracted, lyophilized and encapsulated at RT under anoxic conditions using the DNAshell technology. In total, 1974 samples from 987 patients were encapsulated. Six and two capsules from each sample were stored in the biobanks of the Nice and Grenoble (France) Hospitals, respectively. In conclusion, DNA maintained at RT allows for the conservation, duplication and durability of collections of interest stored in biobanks. This is a low-cost and safe technology that requires a limited amount of space and has a low environmental impact.

Keywords: DNA; biobank; genomic; international networks; lung cancer; personalized medicine; research projects; security; sustainability; tumor tissues

References

  1. Ann Occup Environ Med. 2016 Mar 15;28:12 - PubMed
  2. Mol Ecol Resour. 2013 Sep;13(5):890-8 - PubMed
  3. Biopreserv Biobank. 2013 Aug;11(4):194-201 - PubMed
  4. Risk Anal. 2017 Aug;37(8):1435-1442 - PubMed
  5. Clin Biochem. 2014 Mar;47(4-5):267-73 - PubMed
  6. Ann Oncol. 2017 Jul 1;28(7):1597-1604 - PubMed
  7. Cancer Treat Rev. 2017 Apr;55:136-149 - PubMed
  8. Yearb Med Inform. 2014 May 22;9:8-13 - PubMed
  9. Arch Pathol Lab Med. 2014 Nov;138(11):1520-30 - PubMed
  10. Diagn Mol Pathol. 1996 Dec;5(4):227-35 - PubMed
  11. Sci Rep. 2017 Aug 21;7(1):8371 - PubMed
  12. Eur J Hum Genet. 2014 Mar;22(3):379-85 - PubMed
  13. Medicine (Baltimore). 2017 Mar;96(10):e6221 - PubMed
  14. Biopreserv Biobank. 2014 Jun;12(3):165-75 - PubMed
  15. CA Cancer J Clin. 2004 Jan-Feb;54(1):8-29 - PubMed
  16. Onco Targets Ther. 2017 Sep 12;10:4527-4534 - PubMed
  17. Biopreserv Biobank. 2015 Oct;13(5):356-62 - PubMed
  18. Cancers (Basel). 2017 Nov 13;9(11):null - PubMed
  19. Arch Pathol Lab Med. 2015 Jun;139(6):812-8 - PubMed
  20. J Natl Cancer Inst Monogr. 2011;2011(42):24-31 - PubMed
  21. Eur Respir Rev. 2016 Dec;25(142):364-371 - PubMed
  22. Semin Cancer Biol. 2017 Nov 21;:null - PubMed
  23. Adv Exp Med Biol. 2016;893:43-57 - PubMed
  24. Biopreserv Biobank. 2017 Dec;15(6):502-511 - PubMed
  25. Brief Bioinform. 2017 Nov 29;:null - PubMed
  26. Ann Transl Med. 2017 Sep;5(18):377 - PubMed
  27. Transl Lung Cancer Res. 2015 Oct;4(5):503-14 - PubMed
  28. Transl Lung Cancer Res. 2017 Oct;6(5):570-587 - PubMed
  29. J Thromb Haemost. 2003 May;1(5):987-91 - PubMed
  30. Biopreserv Biobank. 2013 Aug;11(4):193 - PubMed
  31. Forensic Sci Int Genet. 2014 Mar;9:81-4 - PubMed
  32. Clin Transl Med. 2015 Dec;4(1):33 - PubMed
  33. Med Mycol. 2006 Aug;44(5):389-98 - PubMed
  34. Cancer Biol Ther. 2017 Jun 3;18(6):359-368 - PubMed
  35. Ann Occup Environ Med. 2016 Mar 16;28:13 - PubMed
  36. Curr Probl Cancer. 2017 Sep - Oct;41(5):328-339 - PubMed
  37. Biopreserv Biobank. 2017 Oct;15(5):475-483 - PubMed
  38. Epigenomics. 2015;7(6):1003-15 - PubMed
  39. Biopreserv Biobank. 2017 Jun;15(3):253-263 - PubMed
  40. Forensic Sci Int Genet. 2012 Jan;6(1):31-40 - PubMed
  41. Biopreserv Biobank. 2013 Aug;11(4):202-5 - PubMed
  42. Biopreserv Biobank. 2013 Aug;11(4):216-20 - PubMed
  43. Virchows Arch. 2017 Oct;471(4):491-500 - PubMed
  44. Clin Biochem. 2015 Oct;48(15):982-7 - PubMed
  45. Nucleic Acids Res. 2010 Mar;38(5):1531-46 - PubMed
  46. Curr Issues Mol Biol. 2010;12(3):135-42 - PubMed
  47. J Thorac Oncol. 2013 Jul;8(7):923-9 - PubMed
  48. Biopreserv Biobank. 2013 Aug;11(4):211-5 - PubMed
  49. Eur J Cancer. 2015 Sep;51(14):1897-903 - PubMed
  50. J Thorac Oncol. 2017 Jul;12(7):1109-1121 - PubMed
  51. Cancer Genomics Proteomics. 2018 Jan-Feb;15(1):41-51 - PubMed
  52. F1000Res. 2017 Feb 22;6:174 - PubMed
  53. Cancer Epidemiol Biomarkers Prev. 2017 Apr;26(4):444-457 - PubMed
  54. Biopreserv Biobank. 2017 Feb;15(1):46-56 - PubMed
  55. Curr Opin Oncol. 2011 Jan;23(1):112-9 - PubMed
  56. Mol Cancer Res. 2018 Feb;16(2):269-278 - PubMed
  57. Arch Pathol Lab Med. 2010 Nov;134(11):1645-58 - PubMed
  58. Biopreserv Biobank. 2013 Aug;11(4):206-10 - PubMed
  59. Biopreserv Biobank. 2016 Apr;14(2):89-98 - PubMed
  60. Expert Rev Anticancer Ther. 2018 Jan;18(1):63-70 - PubMed
  61. J Clin Microbiol. 2010 Nov;48(11):4260-2 - PubMed
  62. Biopreserv Biobank. 2017 Apr;15(2):85-92 - PubMed
  63. Lung Cancer. 2016 Aug;98:51-58 - PubMed
  64. J Thorac Oncol. 2015 Sep;10(9):1243-1260 - PubMed
  65. J Pathol. 2018 Apr;244(5):512-524 - PubMed
  66. Front Oncol. 2017 Sep 01;7:194 - PubMed
  67. Ann Transl Med. 2017 Sep;5(18):376 - PubMed
  68. Nature. 2012 Jun 06;486(7401):141-6 - PubMed
  69. Ann Transl Med. 2016 Apr;4(8):150 - PubMed
  70. Lung Cancer. 2017 Nov;113:18-29 - PubMed
  71. Biopreserv Biobank. 2011 Mar;9(1):47-50 - PubMed
  72. Oncotarget. 2017 Jan 17;8(3):4313-4329 - PubMed
  73. ScientificWorldJournal. 2014;2014:712826 - PubMed
  74. Biopreserv Biobank. 2015 Feb;13(1):49-55 - PubMed
  75. Eur Respir Rev. 2017 Nov 15;26(146):null - PubMed
  76. Exp Ther Med. 2017 Sep;14(3):2683-2688 - PubMed
  77. PLoS One. 2014 Nov 07;9(11):e111827 - PubMed
  78. Biopreserv Biobank. 2014 Jun;12(3):176-83 - PubMed

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