Display options
Cite
Squaiella-Baptistão CC, Magnoli FC, Marcelino JR, et al. Quality of horse F(ab'). J Venom Anim Toxins Incl Trop Dis. 2018;24:16doi: 10.1186/s40409-018-0153-z.
Squaiella-Baptistão, C. C., Magnoli, F. C., Marcelino, J. R., Sant'Anna, O. A., & Tambourgi, D. V. (2018). Quality of horse F(ab'). The journal of venomous animals and toxins including tropical diseases, 2416. https://doi.org/10.1186/s40409-018-0153-z
Squaiella-Baptistão, Carla Cristina, et al. "Quality of horse F(ab')." The journal of venomous animals and toxins including tropical diseases vol. 24 (2018): 16. doi: https://doi.org/10.1186/s40409-018-0153-z
Squaiella-Baptistão CC, Magnoli FC, Marcelino JR, Sant'Anna OA, Tambourgi DV. Quality of horse F(ab'). J Venom Anim Toxins Incl Trop Dis. 2018 Jun 18;24:16. doi: 10.1186/s40409-018-0153-z. eCollection 2018. PMID: 29946337; PMCID: PMC6006770.
Copy Download .nbib Format: NLM
Share it on

J Venom Anim Toxins Incl Trop Dis. 2018 Jun 18;24:16. doi: 10.1186/s40409-018-0153-z. eCollection 2018.

Quality of horse F(ab').

The journal of venomous animals and toxins including tropical diseases

Carla Cristina Squaiella-Baptistão, Fábio Carlos Magnoli, José Roberto Marcelino, Osvaldo Augusto Sant'Anna, Denise V Tambourgi

Affiliations

  1. 1Laboratório de Imunoquímica, Instituto Butantan, Av. Vital Brazil, 1500, São Paulo, SP CEP 05503-900 Brazil.
  2. 2Seção de Processamento de Plasmas Hiperimunes, Instituto Butantan, Av. Vital Brazil, 1500, São Paulo, SP CEP 05503-900 Brazil.

PMID: 29946337 PMCID: PMC6006770 DOI: 10.1186/s40409-018-0153-z

Abstract

BACKGROUND: Among other applications, immunotherapy is used for the post-exposure treatment and/or prophylaxis of important infectious diseases, such as botulism, diphtheria, tetanus and rabies. The effectiveness of serum therapy is widely proven, but improvements on the immunoglobulin purification process and on the quality control are necessary to reduce the amount of protein aggregates. These may trigger adverse reactions in patients by activating the complement system and inducing the generation of anaphylatoxins. Herein, we used immunochemical methods to predict the quality of horse F(ab')

METHODS: Samples were submitted to protein quantification, SDS-PAGE, Western blot analysis and molecular exclusion chromatography. The anticomplementary activity was determined in vitro by detecting the production of C5a/C5a desArg, the most potent anaphylatoxin. Data were analyzed by one-way ANOVA followed by Tukey's post-test, and differences were considered statistically significant when

RESULTS: Horse F(ab')

CONCLUSIONS: Anti-botulinum AB (bivalent), anti-diphtheric, antitetanic and anti-rabies horse F(ab')

Keywords: Anti-rabies; Antitoxins; Complement system; F(ab’)2 fragment; Heterologous immunoglobulin; Protein profile

Conflict of interest statement

This study and the respective informed consent form were approved by the National Commission on Research Ethics – Research Ethics Committee of the Albert Einstein Hospital (CAAE02001612.6.0000.0071).

References

  1. Curr Infect Dis Rep. 2016 Nov;18(11):38 - PubMed
  2. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
  3. Toxicon. 1994 Jun;32(6):725-34 - PubMed
  4. J Venom Anim Toxins Incl Trop Dis. 2018 Feb 28;24:7 - PubMed
  5. Cell Tissue Res. 2011 Jan;343(1):227-35 - PubMed
  6. Biologicals. 2002 Jun;30(2):143-51 - PubMed
  7. Viruses. 2016 Oct 28;8(11): - PubMed
  8. Toxicon. 2012 Nov;60(6):1022-9 - PubMed
  9. Toxicon. 2001 Jun;39(6):793-801 - PubMed
  10. Expert Opin Drug Discov. 2017 May;12 (5):497-510 - PubMed
  11. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 - PubMed
  12. Clin Toxicol (Phila). 2014 Jul;52(6):594-603 - PubMed
  13. Toxins (Basel). 2010 Nov;2(11):2622-44 - PubMed
  14. J Venom Anim Toxins Incl Trop Dis. 2017 Mar 16;23 :14 - PubMed
  15. Immunobiology. 2012 Feb;217(2):225-34 - PubMed
  16. Clin Microbiol Rev. 2000 Oct;13(4):602-14 - PubMed
  17. Am J Trop Med Hyg. 2014 Mar;90(3):574-84 - PubMed
  18. Toxicon. 1999 Jun;37(6):895-908 - PubMed
  19. Anal Biochem. 1981 Nov 1;117(2):307-10 - PubMed
  20. Vaccine. 2009 Dec 10;28(1):14-20 - PubMed
  21. Toxicon. 2009 May;53(6):609-15 - PubMed

Publication Types