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Haugen S, He J, Sundaresan A, et al. Adiponectin Reduces Bone Stiffness: Verified in a Three-Dimensional Artificial Human Bone Model . Front Endocrinol (Lausanne). 2018;9:236doi: 10.3389/fendo.2018.00236.
Haugen, S., He, J., Sundaresan, A., Stunes, A. K., Aasarød, K. M., Tiainen, H., Syversen, U., Skallerud, B., & Reseland, J. E. (2018). Adiponectin Reduces Bone Stiffness: Verified in a Three-Dimensional Artificial Human Bone Model . Frontiers in endocrinology, 9236. https://doi.org/10.3389/fendo.2018.00236
Haugen, Sigrid, et al. "Adiponectin Reduces Bone Stiffness: Verified in a Three-Dimensional Artificial Human Bone Model ." Frontiers in endocrinology vol. 9 (2018): 236. doi: https://doi.org/10.3389/fendo.2018.00236
Haugen S, He J, Sundaresan A, Stunes AK, Aasarød KM, Tiainen H, Syversen U, Skallerud B, Reseland JE. Adiponectin Reduces Bone Stiffness: Verified in a Three-Dimensional Artificial Human Bone Model . Front Endocrinol (Lausanne). 2018 May 14;9:236. doi: 10.3389/fendo.2018.00236. eCollection 2018. PMID: 29867768; PMCID: PMC5960720.
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Front Endocrinol (Lausanne). 2018 May 14;9:236. doi: 10.3389/fendo.2018.00236. eCollection 2018.

Adiponectin Reduces Bone Stiffness: Verified in a Three-Dimensional Artificial Human Bone Model .

Frontiers in endocrinology

Sigrid Haugen, Jianying He, Alamelu Sundaresan, Astrid Kamilla Stunes, Kristin Matre Aasarød, Hanna Tiainen, Unni Syversen, Bjørn Skallerud, Janne Elin Reseland

Affiliations

  1. Department of Biomaterials, Institute for Clinical Dentistry, University of Oslo, Oslo, Norway.
  2. Department of Structural Engineering, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
  3. Department of Biology, Texas Southern University, Houston, TX, United States.
  4. Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
  5. St. Olav's University Hospital, Trondheim, Norway.
  6. Department of Endocrinology, St. Olav's University Hospital, Trondheim, Norway.

PMID: 29867768 PMCID: PMC5960720 DOI: 10.3389/fendo.2018.00236

Abstract

Primary human osteoblasts and osteoclasts incubated in a rotating coculture system without any scaffolding material, form bone-like tissue that may be used to evaluate effects of various compounds on mechanical strength. Circulating adiponectin has been found to be negatively associated with BMD and strength and was therefore assessed in this system. Osteospheres of human osteoblasts and osteoclasts were generated with and without adiponectin. The osteospheres were scanned using micro-computed tomography, the mechanical properties were tested by flat punch compression using nanoindentation equipment, and the cellular morphology characterized by microscopy. The association between autologously produced adiponectin and biomechanical properties was further evaluated by quantitation of adiponectin levels using quantitative polymerase chain reaction (qPCR) and immunoassays, and identification of stiffness by bending test of rat femurs. The molecular mechanisms were examined

Keywords: adiponectin; bone remodeling; bone stiffness; osteospheres; three-dimensional culturing system

References

  1. Bone. 2014 Jul;64:147-54 - PubMed
  2. Nature. 1964 Nov 21;204:793-4 - PubMed
  3. Stem Cells. 2009 Sep;27(9):2254-62 - PubMed
  4. J Anat. 2015 Dec;227(6):746-56 - PubMed
  5. Biochem Biophys Res Commun. 1999 Apr 2;257(1):79-83 - PubMed
  6. Endocrine. 2014 Dec;47(3):845-53 - PubMed
  7. BMC Endocr Disord. 2011 May 26;11:11 - PubMed
  8. Acta Biomater. 2013 Aug;9(8):7908-16 - PubMed
  9. Diabetologia. 2017 Oct;60(10 ):1922-1930 - PubMed
  10. Osteoporos Int. 2006;17(3):319-36 - PubMed
  11. Life Sci. 2016 May 1;152:82-93 - PubMed
  12. J Cell Biochem. 2006 Sep 1;99(1):196-208 - PubMed
  13. Eur Cell Mater. 2011 Jan 25;21:80-93 - PubMed
  14. J Biochem. 1996 Oct;120(4):803-12 - PubMed
  15. J Bone Miner Res. 2006 Oct;21(10):1648-56 - PubMed
  16. Biochem Biophys Res Commun. 2005 Jun 3;331(2):520-6 - PubMed
  17. J Recept Signal Transduct Res. 2013 Oct;33(5):291-7 - PubMed
  18. BMC Cell Biol. 2007 Nov 29;8:51 - PubMed
  19. J Mech Behav Biomed Mater. 2016 Jul;60:401-415 - PubMed
  20. J Orthop Surg Res. 2011 Jun 15;6:30 - PubMed
  21. J Invest Dermatol. 2013 Mar;133(3):812-821 - PubMed
  22. Clin Oral Investig. 2013 Jul;17(6):1547-55 - PubMed
  23. Osteoporos Int. 2014 Nov;25(11):2599-607 - PubMed
  24. Am J Physiol Endocrinol Metab. 2014 Jun 15;306(12 ):E1418-30 - PubMed
  25. JAMA. 2009 Jul 8;302(2):179-88 - PubMed
  26. Metab Syndr Relat Disord. 2008 Jun;6(2):87-102 - PubMed
  27. Acta Orthop Scand. 1978 Dec;49(6):512-8 - PubMed
  28. Bone. 2004 Oct;35(4):842-9 - PubMed
  29. Calcif Tissue Int. 2016 Jan;98 (1):85-93 - PubMed
  30. J Clin Endocrinol Metab. 2004 Jan;89(1):87-90 - PubMed
  31. Endocrinology. 2016 Sep;157(9):3550-61 - PubMed
  32. Calcif Tissue Int. 1994 Dec;55(6):436-42 - PubMed
  33. Nat Methods. 2012 Jul;9(7):671-5 - PubMed
  34. Proc Soc Exp Biol Med. 1952 Jul;80(3):474-9 - PubMed
  35. Acta Biomater. 2014 Dec;10 (12 ):5139-5147 - PubMed
  36. J Dent Res. 2010 Dec;89(12):1333-48 - PubMed
  37. Biomaterials. 2009 Oct;30(29):5376-84 - PubMed
  38. Exp Cell Res. 2005 Sep 10;309(1):99-109 - PubMed
  39. Horm Metab Res. 2005 May;37(5):297-302 - PubMed
  40. Nat Med. 2001 Aug;7(8):941-6 - PubMed
  41. Assay Drug Dev Technol. 2014 May;12(4):207-18 - PubMed
  42. Biochim Biophys Acta. 2011 Mar;1810(3):239-50 - PubMed
  43. BMC Endocr Disord. 2009 Mar 30;9:10 - PubMed
  44. J Biol Chem. 1995 Nov 10;270(45):26746-9 - PubMed
  45. Eur Cell Mater. 2015 May 15;29:250-66; discussion 266-7 - PubMed
  46. Cell Metab. 2013 Jun 4;17 (6):901-15 - PubMed
  47. PPAR Res. 2011;2011:436358 - PubMed
  48. J Biol Chem. 2011 Apr 8;286(14 ):12542-53 - PubMed
  49. Acta Biomater. 2012 Feb;8(2):734-43 - PubMed
  50. J Biol Chem. 1996 May 3;271(18):10697-703 - PubMed
  51. Physiol Rev. 1996 Apr;76(2):593-629 - PubMed
  52. Clin Oral Implants Res. 2014 Mar;25(3):385-394 - PubMed
  53. Biochem Biophys Res Commun. 2012 Aug 31;425(3):556-9 - PubMed
  54. Endocrinology. 2009 Aug;150(8):3603-10 - PubMed
  55. Bone. 2010 Dec;47(6):1001-5 - PubMed
  56. Bone Res. 2015 Sep 15;3:15026 - PubMed
  57. Int J Mol Sci. 2016 Jul 14;17 (7): - PubMed
  58. J Clin Invest. 2006 Jul;116(7):1784-92 - PubMed
  59. Endocrinology. 2002 Mar;143(3):998-1007 - PubMed
  60. Calcif Tissue Int. 2017 May;100(5):486-499 - PubMed

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