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Lubośny M, Przyłucka A, Śmietanka B, et al. Actively transcribed and expressed . PeerJ. 2018;6:e4897doi: 10.7717/peerj.4897.
Lubośny, M., Przyłucka, A., Śmietanka, B., Breton, S., & Burzyński, A. (2018). Actively transcribed and expressed . PeerJ, 6e4897. https://doi.org/10.7717/peerj.4897
Lubośny, Marek, et al. "Actively transcribed and expressed ." PeerJ vol. 6 (2018): e4897. doi: https://doi.org/10.7717/peerj.4897
Lubośny M, Przyłucka A, Śmietanka B, Breton S, Burzyński A. Actively transcribed and expressed . PeerJ. 2018 Jun 08;6:e4897. doi: 10.7717/peerj.4897. eCollection 2018. PMID: 29900071; PMCID: PMC5995098.
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PeerJ. 2018 Jun 08;6:e4897. doi: 10.7717/peerj.4897. eCollection 2018.

Actively transcribed and expressed .

PeerJ

Marek Lubośny, Aleksandra Przyłucka, Beata Śmietanka, Sophie Breton, Artur Burzyński

Affiliations

  1. Department of Genetics and Marine Biotechnology, Institute of Oceanology Polish Academy of Sciences, Sopot, Poland.
  2. Department of Biological Sciences, Université de Montréal, Montréal, Québec, Canada.

PMID: 29900071 PMCID: PMC5995098 DOI: 10.7717/peerj.4897

Abstract

BACKGROUND: Animal mitochondrial genomes typically encode 37 genes: 13 proteins, 22 tRNAs and two rRNAs. However, many species represent exceptions to that rule. Bivalvia along with Nematoda and Platyhelminthes are often suspected to fully or partially lack the ATP synthase subunit 8 (

RESULTS: Here we demonstrate that

CONCLUSION: Many organisms (e.g., Bivalvia along with Nematoda and Platyhelminthes) are considered to be lacking certain mitochondrial genes often only based on poor similarity between protein coding gene sequences in genetically closed species. In some situations, this may lead to the inference that the ATP8 gene is absent, when it is in fact present, but highly divergent. This shows how important complementary role protein-based approaches, such as those in the present study, can provide to bioinformatic, genomic studies (i.e., ability to confirm the presence of a gene).

Keywords: ATP8; Bivalvia; Blue Native; Doubly uniparental inheritance; Mitochondrial DNA; Western blot

Conflict of interest statement

The authors declare there are no competing interests.

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