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Moreira LN, Silva JF, Silva GC, et al. Activation of eNOS by D-pinitol Induces an Endothelium-Dependent Vasodilatation in Mouse Mesenteric Artery. Front Pharmacol. 2018;9:528doi: 10.3389/fphar.2018.00528.
Moreira, L. N., Silva, J. F., Silva, G. C., Lemos, V. S., & Cortes, S. F. (2018). Activation of eNOS by D-pinitol Induces an Endothelium-Dependent Vasodilatation in Mouse Mesenteric Artery. Frontiers in pharmacology, 9528. https://doi.org/10.3389/fphar.2018.00528
Moreira, Luciana N, et al. "Activation of eNOS by D-pinitol Induces an Endothelium-Dependent Vasodilatation in Mouse Mesenteric Artery." Frontiers in pharmacology vol. 9 (2018): 528. doi: https://doi.org/10.3389/fphar.2018.00528
Moreira LN, Silva JF, Silva GC, Lemos VS, Cortes SF. Activation of eNOS by D-pinitol Induces an Endothelium-Dependent Vasodilatation in Mouse Mesenteric Artery. Front Pharmacol. 2018 May 22;9:528. doi: 10.3389/fphar.2018.00528. eCollection 2018. PMID: 29872397; PMCID: PMC5972298.
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Front Pharmacol. 2018 May 22;9:528. doi: 10.3389/fphar.2018.00528. eCollection 2018.

Activation of eNOS by D-pinitol Induces an Endothelium-Dependent Vasodilatation in Mouse Mesenteric Artery.

Frontiers in pharmacology

Luciana N Moreira, Josiane F Silva, Grazielle C Silva, Virgínia S Lemos, Steyner F Cortes

Affiliations

  1. Department of Pharmacology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  2. Department of Physiology and Biophysics, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

PMID: 29872397 PMCID: PMC5972298 DOI: 10.3389/fphar.2018.00528

Abstract

D-pinitol is a cyclitol present in several edible plant species and extensively investigated for the treatment of metabolic diseases in humans, as food supplement, and demonstrated protective effects in the cardiovascular system. For these reasons, the present work aimed at investigating the mechanisms involved in the vascular effects of D-pinitol in mouse mesenteric artery. Mesenteric arteries from male C57BL/6 mice were mounted in a wire myograph. Nitrite was measured by the 2,3-diaminonaphthalene (DAN) method. Protein expression and phosphorylation were measured by Western blot. The systolic blood pressure (SBP) was measured by tail-cuff plethysmography. D-pinitol induced a concentration-dependent vasodilatation in endothelium-intact, but not in endothelium-denuded arteries. Nω-Nitro-L-arginine methyl ester (300 μM) abolished the effect of D-pinitol, while 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ; 10 μM) shifted the concentration-response curve to the right. KN-93 (1 μM) blunted the vasodilator effect of D-pinitol, but H-89 (0.1 μM) did not change it. 1-[2-(Trifluoromethyl) phenyl]imidazole (300 μM), indomethacin (10 μM), celecoxib (5 μM), wortmannin (1 μM), ruthenium red (10 μM), tiron (10 μM), MnTMPyP (30 μM), MPP (0.1 μM), PHTPP (0.1 μM), and atropine (1 μM) did not change the effect of D-pinitol. D-pinitol increased the concentration of nitrite, which was inhibited by L-NAME and calmidazolium (10 μM). D-pinitol increased the phosphorylation level of eNOS activation site at Ser

Keywords: D-pinitol; calcium-calmodulin complex; endothelium; mesenteric artery; nitric oxide synthase

References

  1. J Physiol. 1988 Apr;398:277-89 - PubMed
  2. Am J Physiol Heart Circ Physiol. 2003 Jun;284(6):H2311-9 - PubMed
  3. Clin Sci (Lond). 2016 Jun 1;130(11):895-906 - PubMed
  4. Angew Chem Int Ed Engl. 2016 Jan 26;55(5):1614-50 - PubMed
  5. Proc Natl Acad Sci U S A. 2006 Jan 3;103(1):218-23 - PubMed
  6. Am J Physiol Heart Circ Physiol. 2008 Dec;295(6):H2503-11 - PubMed
  7. Diabetes Care. 2000 Jul;23(7):1000-5 - PubMed
  8. Hypertension. 2003 Apr;41(4):879-81 - PubMed
  9. Eur J Obstet Gynecol Reprod Biol. 2015 Dec;195:72-6 - PubMed
  10. Eur J Clin Nutr. 2005 Mar;59(3):456-8 - PubMed
  11. Biochem Biophys Res Commun. 2002 Jan 25;290(3):909-13 - PubMed
  12. Biochemistry. 1996 Nov 19;35(46):14651-8 - PubMed
  13. Nature. 1999 Jun 10;399(6736):597-601 - PubMed
  14. FEBS Lett. 1990 Jun 4;265(1-2):133-6 - PubMed
  15. J Cardiovasc Pharmacol. 2005 Sep;46(3):274-9 - PubMed
  16. Biochem J. 1993 Dec 1;296 ( Pt 2):297-301 - PubMed
  17. J Smooth Muscle Res. 2003 Dec;39(6):249-67 - PubMed
  18. Eur J Pharmacol. 2010 Sep 25;643(2-3):260-6 - PubMed
  19. Nature. 1999 Jun 10;399(6736):601-5 - PubMed
  20. Circulation. 2001 Mar 13;103(10):1410-5 - PubMed
  21. Ann Nutr Metab. 2012;60(1):1-5 - PubMed
  22. Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6252-6 - PubMed
  23. Circ Res. 2009 Jul 17;105(2):114-27 - PubMed
  24. J Biol Chem. 1999 Oct 15;274(42):30101-8 - PubMed
  25. Circ Res. 2001 Jun 8;88(11):E68-75 - PubMed
  26. Br J Pharmacol. 2000 Aug;130(8):1944-8 - PubMed
  27. Atherosclerosis. 2006 Apr;185(2):227-39 - PubMed
  28. J Exp Med. 2008 Sep 1;205(9):2053-63 - PubMed
  29. Cell Calcium. 2005 Sep-Oct;38(3-4):233-52 - PubMed
  30. Peptides. 2003 Mar;24(3):455-62 - PubMed
  31. Nat Rev Cardiol. 2012 May 22;9(8):439-53 - PubMed
  32. Curr Diab Rep. 2016 Mar;16(3):30 - PubMed
  33. Jpn J Pharmacol. 2001 Aug;86(4):369-75 - PubMed
  34. J Pharmacol Exp Ther. 2011 Mar;336(3):701-8 - PubMed
  35. Biosci Biotechnol Biochem. 2010;74(5):1062-7 - PubMed
  36. Eur Heart J. 2012 Apr;33(7):829-37, 837a-837d - PubMed
  37. J Nutr. 2004 Nov;134(11):2998-3003 - PubMed
  38. Annu Rev Physiol. 2006;68:685-717 - PubMed
  39. Circ Res. 2007 Feb 2;100(2):158-73 - PubMed
  40. Hypertension. 2009 Mar;53(3):532-8 - PubMed
  41. Biochimie. 2013 Oct;95(10):1811-27 - PubMed
  42. Pflugers Arch. 2010 May;459(6):793-806 - PubMed
  43. J Med Food. 2006 Summer;9(2):182-6 - PubMed

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