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Mohammad NA, Mastuki MF, Al-Mekhlafi HM, et al. Comparative Study of Wheatley's Trichrome Stain and In-vitro Culture against PCR Assay for the Diagnosis of . Iran J Parasitol. 2018;13(1):127-136
Mohammad, N. A., Mastuki, M. F., Al-Mekhlafi, H. M., Moktar, N., & Anuar, T. S. (2018). Comparative Study of Wheatley's Trichrome Stain and In-vitro Culture against PCR Assay for the Diagnosis of . Iranian journal of parasitology, 13(1), 127-136.
Mohammad, Nabilah Amelia, et al. "Comparative Study of Wheatley's Trichrome Stain and In-vitro Culture against PCR Assay for the Diagnosis of ." Iranian journal of parasitology vol. 13,1 (2018): 127-136.
Mohammad NA, Mastuki MF, Al-Mekhlafi HM, Moktar N, Anuar TS. Comparative Study of Wheatley's Trichrome Stain and In-vitro Culture against PCR Assay for the Diagnosis of . Iran J Parasitol. 2018 Jan-Mar;13(1):127-136. PMID: 29963095; PMCID: PMC6019595.
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Iran J Parasitol. 2018 Jan-Mar;13(1):127-136.

Comparative Study of Wheatley's Trichrome Stain and In-vitro Culture against PCR Assay for the Diagnosis of .

Iranian journal of parasitology

Nabilah Amelia Mohammad, Mohd Fahmi Mastuki, Hesham M Al-Mekhlafi, Norhayati Moktar, Tengku Shahrul Anuar

Affiliations

  1. Center of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam Campus, Selangor, Malaysia.
  2. Endemic and Tropical Diseases Unit, Medical Research Center, Jazan University, Jazan, Kingdom of Saudi Arabia.
  3. Dept. of Parasitology, Faculty of Medicine and Health Sciences, Sana'a University, Sana'a, Yemen.
  4. Dept. of Pre-Clinical Sciences, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Sungai Long Campus, Selangor, Malaysia.
  5. Integrative Pharmacogenomics Institute, Universiti Teknologi MARA, Puncak Alam Campus, Selangor, Malaysia.

PMID: 29963095 PMCID: PMC6019595

Abstract

BACKGROUND: This study evaluated the performance of routine permanent stain and cultivation method in comparison with polymerase chain reaction assay as the reference technique to detect

METHODS: A cross-sectional study was conducted among aboriginal populations that reside in Pahang, Peninsular Malaysia in Feb to Mar 2015. A total of 359 stool samples were examined using Wheatley's trichrome stain, in-vitro cultivation in Jones' medium and PCR assay. Positive amplicons were subjected to sequencing and phylogenetic analysis.

RESULTS: Fifty-six (15.6%) samples were detected positive with

CONCLUSION: The combination of microscopic technique is highly recommended to be used as a screening method for the diagnosis of

Keywords: Blastocystis; In-vitro culture; PCR; Trichrome stain

Conflict of interest statement

Conflict of interest The authors declare that there is no conflict of interest.

References

  1. PLoS One. 2010 Nov 18;5(11):e15484 - PubMed
  2. Trop Biomed. 2010 Apr;27(1):79-88 - PubMed
  3. Parasitol Res. 2008 Mar;102(4):663-70 - PubMed
  4. Asian Pac J Trop Med. 2013 Oct;6(10):780-4 - PubMed
  5. Parasitol Int. 2007 Dec;56(4):281-6 - PubMed
  6. J Parasitol. 2006 Oct;92(5):1081-7 - PubMed
  7. Diagn Microbiol Infect Dis. 2007 Nov;59(3):303-7 - PubMed
  8. Parasit Vectors. 2012 Jun 28;5:130 - PubMed
  9. J Protozool. 1981 Nov;28(4):483-5 - PubMed
  10. Mol Biol Evol. 2013 Dec;30(12):2725-9 - PubMed
  11. Ann Trop Med Parasitol. 2002 Dec;96(8):803-7 - PubMed
  12. Parasitology. 2007 Mar;134(Pt 3):359-67 - PubMed
  13. Parasitol Res. 2010 Jan;106(2):505-11 - PubMed
  14. Parasit Vectors. 2013 Feb 22;6:40 - PubMed
  15. Ann Trop Med Parasitol. 2008 Apr;102(3):271-4 - PubMed
  16. Protist. 2013 Jul;164(4):497-509 - PubMed
  17. Ann Trop Med Parasitol. 1946 Jul;40:130-40 - PubMed
  18. Clin Microbiol Rev. 2008 Oct;21(4):639-65 - PubMed
  19. Eur J Clin Microbiol Infect Dis. 2004 Jun;23(6):509-11 - PubMed
  20. Trop Parasitol. 2015 Jan-Jun;5(1):36-41 - PubMed
  21. BMC Infect Dis. 2013 Aug 23;13:389 - PubMed
  22. Trop Med Int Health. 1997 Aug;2(8):771-8 - PubMed
  23. J Microbiol Methods. 2013 Mar;92(3):344-8 - PubMed
  24. J Clin Microbiol. 1990 May;28(5):1085-6 - PubMed
  25. Parasit Vectors. 2008 Oct 21;1(1):40 - PubMed
  26. J Microbiol Methods. 2012 Oct;91(1):174-8 - PubMed
  27. Mikrobiyol Bul. 2015 Jan;49(1):85-97 - PubMed
  28. Parasit Vectors. 2015 Sep 17;8:465 - PubMed
  29. Parasit Vectors. 2013 Oct 11;6(1):295 - PubMed
  30. Am J Trop Med Hyg. 2011 Feb;84(2):308-12 - PubMed
  31. Parasitol Res. 2008 Dec;104(1):85-93 - PubMed
  32. Parasitol Res. 2004 Aug;93(6):445-7 - PubMed
  33. Korean J Parasitol. 2015 Feb;53(1):13-9 - PubMed

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