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Iran J Microbiol. 2018 Apr;10(2):90-97.

Assessment of disinfectant and antibiotic susceptibility patterns and multi-locus variable number tandem repeat analysis of .

Iranian journal of microbiology

Parisa Asadollahi, Fereshteh Jabalameli, Reza Beigverdi, Mohammad Emaneini

Affiliations

  1. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

PMID: 29997748 PMCID: PMC6039456

Abstract

BACKGROUND AND OBJECTIVES: Variable number tandem repeat (VNTR) patterns and resistance against three commonly used hospital disinfectants [0.5% (w/w) chlorhexidine digluconate (CHG) and 75% (w/w) alcohol (A), CHG-A; Quaternary ammonium compounds (QACs) and biguanides (B), QAC-B; and 70% (w/w) isopropanol (ISP) and 0.25% (w/w) QACs, ISP-QAC], as well as frequently used antibiotics, were evaluated among 115

MATERIALS AND METHODS: Multi-locus variable number tandem repeat analysis (MLVA) was performed using primers targeting 5 VNTR loci on the genome of

RESULTS: Out of the 115 isolates, 115 (100%) and 113 (98.3%) were susceptible to linezolid and quinupristin/dalfopristin, respectively. A total of 55.7% of the isolates were found to be multidrug resistant (MDR). All isolates had MICs of CHG-A and ISP-QAC of 8 folds lower and MIC of QAC-B 6 folds lower than that suggested by the manufacturers. The genes

CONCLUSION: MLVA typing of

Keywords: Blood cultures; Disinfectants; Multidrug resistant; Staphylococcus epidermidis; VNTR

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