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Maglione M, Salvador E, Ruaro ME, et al. Bone regeneration with adipose derived stem cells in a rabbit model. J Biomed Res. 2018;doi: 10.7555/JBR.32.20160066.
Maglione, M., Salvador, E., Ruaro, M. E., Melato, M., Tromba, G., Angerame, D., & Bevilacqua, L. (2018). Bone regeneration with adipose derived stem cells in a rabbit model. Journal of biomedical research, . https://doi.org/10.7555/JBR.32.20160066
Maglione, Michele, et al. "Bone regeneration with adipose derived stem cells in a rabbit model." Journal of biomedical research vol. (2018). doi: https://doi.org/10.7555/JBR.32.20160066
Maglione M, Salvador E, Ruaro ME, Melato M, Tromba G, Angerame D, Bevilacqua L. Bone regeneration with adipose derived stem cells in a rabbit model. J Biomed Res. 2018 Jul 11; doi: 10.7555/JBR.32.20160066. Epub 2018 Jul 11. PMID: 30007953; PMCID: PMC6352878.
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J Biomed Res. 2018 Jul 11; doi: 10.7555/JBR.32.20160066. Epub 2018 Jul 11.

Bone regeneration with adipose derived stem cells in a rabbit model.

Journal of biomedical research

Michele Maglione, Enrico Salvador, Maria E Ruaro, Mauro Melato, Giuliana Tromba, Daniele Angerame, Lorenzo Bevilacqua

Affiliations

  1. Department of Medical Sciences, University of Trieste, Trieste 34125, Italy.
  2. SISSA-International School for Advanced Studies, Trieste 34136, Italy.
  3. Department of Pathology and Legal Medicine, University of Trieste, Trieste 34125, Italy.
  4. Elettra-Sincrotrone Trieste S.C.p.A., Trieste 34149, Italy.

PMID: 30007953 PMCID: PMC6352878 DOI: 10.7555/JBR.32.20160066

Abstract

It has been shown that stem cells are able to calcify both in vitro and in vivo once implanted under the skin, if conveniently differentiated. Nowadays, however, a study on their efficiency in osseous regeneration does not exist in scientific literature and this very task is the real aim of the present experimentation. Five different defects of 6 mm in diameter and 2 mm in depth were created in the calvaria of 8 white New Zealand rabbits. Four defects were regenerated using 2 different conveniently modified scaffolds (Bio-Oss® Block and Bio-Oss Collagen®, Geistlich), with and without the aid of stem cells. After the insertion, the part was covered with a collagen membrane fixed by 5 modified titan pins (Altapin®). The defect in the front was left empty on purpose as an internal control to each animal. Two animals were sacrificed respectively after 2, 4, 6, 10 weeks. The samples were evaluated with micro-CT and histological analysis. Micro-CT analysis revealed that the quantity of new bone for samples with Bio-Oss® Block and stem cells was higher than for samples with Bio-Oss® Block alone. Histological analysis showed that regeneration occurred in an optimal way in every sample treated with scaffolds. The findings indicated that the use of adult stem cells combined with scaffolds accelerated some steps in normal osseous regeneration.

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