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Abbas AM, Abd El-Moaty DAM, Zaki ESA, et al. Use of molecular biology tools for rapid identification and characterization of . Vet World. 2018;11(7):1006-1014doi: 10.14202/vetworld.2018.1006-1014.
Abbas, A. M., Abd El-Moaty, D. A. M., Zaki, E. S. A., El-Sergany, E. F., El-Sebay, N. A., Fadl, H. A., & Samy, A. A. (2018). Use of molecular biology tools for rapid identification and characterization of . Veterinary world, 11(7), 1006-1014. https://doi.org/10.14202/vetworld.2018.1006-1014
Abbas, Ashraf M, et al. "Use of molecular biology tools for rapid identification and characterization of ." Veterinary world vol. 11,7 (2018): 1006-1014. doi: https://doi.org/10.14202/vetworld.2018.1006-1014
Abbas AM, Abd El-Moaty DAM, Zaki ESA, El-Sergany EF, El-Sebay NA, Fadl HA, Samy AA. Use of molecular biology tools for rapid identification and characterization of . Vet World. 2018 Jul;11(7):1006-1014. doi: 10.14202/vetworld.2018.1006-1014. Epub 2018 Jul 29. PMID: 30147273; PMCID: PMC6097567.
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Vet World. 2018 Jul;11(7):1006-1014. doi: 10.14202/vetworld.2018.1006-1014. Epub 2018 Jul 29.

Use of molecular biology tools for rapid identification and characterization of .

Veterinary world

Ashraf M Abbas, Dalia A M Abd El-Moaty, Eman S A Zaki, Elham F El-Sergany, Nadine A El-Sebay, Hala A Fadl, A A Samy

Affiliations

  1. Genetic Engineering Research Department, Veterinary Serum and Vaccine Research Institute, Cairo Egypt.
  2. Aerobic Bacterial Vaccine Research Department, Veterinary Serum and Vaccine Research Institute, Cairo Egypt.
  3. Anaerobic Bacterial Vaccine Research Department, Veterinary Serum and Vaccine Research Institute, Cairo Egypt.
  4. Department of Microbiology and Immunology, Veterinary Division, National Research Center, Dokki, Egypt.

PMID: 30147273 PMCID: PMC6097567 DOI: 10.14202/vetworld.2018.1006-1014

Abstract

AIM: This study aimed to create rapid characterization and genotyping of

MATERIALS AND METHODS: Thirty bacterial isolates were characterized by capsular and somatic identification using conventional procedure followed by multiplex polymerase chain reaction (PCR), restriction endonucleases analysis (REA), and finally confirmed by sequence analysis. Two local vaccine strains and two field isolates were identified as PM Type A and B.

RESULTS: A total of 30 isolates were found positive for PM either morphologically and biochemically; however, multiplex PCR technique identified only 22 isolates as

CONCLUSION: PCR and REA could confirm the identity of PM and provide a rapid and reliable characterization in comparison with biochemical analysis and conventional serotyping that may take up to 2 weeks. Hence, they can reduce the time needed for polyvalent vaccine production and when the reference antisera are unavailable. Moreover, the identity of Omp-H for vaccine and field strains may provide better data to control Pasteurellosis in Egypt.

Keywords: Pasteurella multocida; multiplex polymerase chain reaction; outer membrane protein H; restriction endonucleases analysis

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