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Alkan İ, Yüksel M, Canat HL, et al. Superoxide Anion Production by the Spermatozoa of Men with Varicocele: Relationship with Varicocele Grade and Semen Parameters. World J Mens Health. 2018;36(3):255-262doi: 10.5534/wjmh.180028.
Alkan, �. �., Yüksel, M., Canat, H. L., Atalay, H. A., Can, O., Özveri, H., & Başar, M. M. (2018). Superoxide Anion Production by the Spermatozoa of Men with Varicocele: Relationship with Varicocele Grade and Semen Parameters. The world journal of men's health, 36(3), 255-262. https://doi.org/10.5534/wjmh.180028
Alkan, İlter, et al. "Superoxide Anion Production by the Spermatozoa of Men with Varicocele: Relationship with Varicocele Grade and Semen Parameters." The world journal of men's health vol. 36,3 (2018): 255-262. doi: https://doi.org/10.5534/wjmh.180028
Alkan İ, Yüksel M, Canat HL, Atalay HA, Can O, Özveri H, Başar MM. Superoxide Anion Production by the Spermatozoa of Men with Varicocele: Relationship with Varicocele Grade and Semen Parameters. World J Mens Health. 2018 Sep;36(3):255-262. doi: 10.5534/wjmh.180028. PMID: 30168299; PMCID: PMC6119845.
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World J Mens Health. 2018 Sep;36(3):255-262. doi: 10.5534/wjmh.180028.

Superoxide Anion Production by the Spermatozoa of Men with Varicocele: Relationship with Varicocele Grade and Semen Parameters.

The world journal of men's health

İlter Alkan, Meral Yüksel, Halil Lütfi Canat, Hasan Anıl Atalay, Osman Can, Hakan Özveri, Mehmet Murad Başar

Affiliations

  1. Department of Urology, Okmeydan? Training and Research Hospital, University of Health Sciences, Istanbul, Turkey. [email protected].
  2. Department of Medical Laboratory, Vocational School of Health-Related Services, Marmara University, Istanbul, Turkey.
  3. Department of Urology, Okmeydan? Training and Research Hospital, University of Health Sciences, Istanbul, Turkey.
  4. Department of Urology & Andrology, School of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey.
  5. Department of Urology & Andrology, Memorial ?i?li Hospital, Istanbul, Turkey.

PMID: 30168299 PMCID: PMC6119845 DOI: 10.5534/wjmh.180028

Abstract

PURPOSE: To investigate the pathophysiological role of superoxide anion and total reactive oxygen species (ROS) production by the spermatozoa of men with varicocele and its relationship with varicocele grade and semen parameters.

MATERIALS AND METHODS: This prospective study included 34 men with grade II-III varicocele, regardless of their fertility status. The control group consisted of 13 healthy men. Semen characteristics were examined according to the 2010 World Health Organization criteria. The swim-up method was used for sperm preparation. Total ROS and superoxide anion production was assayed by luminol- and lucigenin-dependent chemiluminescence (CL), respectively.

RESULTS: The men with varicocele had significantly higher total ROS and superoxide anion levels than the healthy control subjects (2.9±0.4 relative light unit (RLU) vs. 2.4±0.1 RLU, p=0.001 for luminol-dependent CL and 2.8±0.4 RLU vs. 2.3±0.2 RLU, p=0.002 for lucigenin-dependent CL). Cases of grade III varicocele had significantly higher superoxide anion and total ROS levels than grade II cases and control subjects (p<0.001). Superoxide anion and total ROS levels were negatively correlated with all semen parameters.

CONCLUSIONS: The superoxide anion levels produced by spermatozoa were significantly higher in varicocele patients than in control subjects. ROS production was related to increased varicocele grade, impaired semen concentration, and abnormal morphology in men with varicocele. Our findings suggest that superoxide anion overproduction may be an important step in the cascade of ROS-related damage to spermatozoa, resulting in impaired semen parameters in patients with varicocele.

Copyright © 2018 Korean Society for Sexual Medicine and Andrology.

Keywords: Oxidative stress; Reactive oxygen species; Spermatozoa; Superoxides; Varicocele

Conflict of interest statement

The authors have no potential conflicts of interest to disclose.

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