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Bekusova VV, Falchuk EL, Okorokova LS, et al. Increased paracellular permeability of tumor-adjacent areas in 1,2-dimethylhydrazine-induced colon carcinogenesis in rats. Cancer Biol Med. 2018;15(3):251-259doi: 10.20892/j.issn.2095-3941.2018.0016.
Bekusova, V. V., Falchuk, E. L., Okorokova, L. S., Kruglova, N. M., Nozdrachev, A. D., & Markov, A. G. (2018). Increased paracellular permeability of tumor-adjacent areas in 1,2-dimethylhydrazine-induced colon carcinogenesis in rats. Cancer biology & medicine, 15(3), 251-259. https://doi.org/10.20892/j.issn.2095-3941.2018.0016
Bekusova, Viktoria V, et al. "Increased paracellular permeability of tumor-adjacent areas in 1,2-dimethylhydrazine-induced colon carcinogenesis in rats." Cancer biology & medicine vol. 15,3 (2018): 251-259. doi: https://doi.org/10.20892/j.issn.2095-3941.2018.0016
Bekusova VV, Falchuk EL, Okorokova LS, Kruglova NM, Nozdrachev AD, Markov AG. Increased paracellular permeability of tumor-adjacent areas in 1,2-dimethylhydrazine-induced colon carcinogenesis in rats. Cancer Biol Med. 2018 Aug;15(3):251-259. doi: 10.20892/j.issn.2095-3941.2018.0016. PMID: 30197792; PMCID: PMC6121046.
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Cancer Biol Med. 2018 Aug;15(3):251-259. doi: 10.20892/j.issn.2095-3941.2018.0016.

Increased paracellular permeability of tumor-adjacent areas in 1,2-dimethylhydrazine-induced colon carcinogenesis in rats.

Cancer biology & medicine

Viktoria V Bekusova, Evgeny L Falchuk, Larisa S Okorokova, Natalia M Kruglova, Alexander D Nozdrachev, Alexander G Markov

Affiliations

  1. Department of Physiology, St. Petersburg State University, St. Petersburg 197183, Russia.
  2. I.P.Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg 199034, Russia.

PMID: 30197792 PMCID: PMC6121046 DOI: 10.20892/j.issn.2095-3941.2018.0016

Abstract

OBJECTIVE: The morphology and functions of the proximal and distal large intestine are not the same. The incidence of colorectal cancer in these regions is also different, as tumors more often appear in the descending colon than in the ascending colon. Inflammatory bowel disease and colorectal cancer can increase transepithelial permeability, which is a sign of reduced intestinal barrier function. However, there is not enough evidence to establish a connection between the difference in colorectal cancer incidence in the proximal and distal colon and intestinal permeability or the effects of carcinogenesis on the barrier properties in various areas of the colon. The aim of the study was to assess the permeability of different segments of the large intestine according to a developed mapping methodology in healthy rats and rats with 1,2-dimethylhydrazine (DMH)-induced colon adenocarcinoma.

METHODS: The short circuit current, the transepithelial electrical resistance and the paracellular permeability to fluorescein of large intestine wall of male Wistar rats were examined in the Ussing chambers. The optical density of the solution from the serosa side to assess the concentration of the diffused fluorescein from mucosa to serosa was analyzed by spectrophotometry. The morphometric and histological studies were performed by optical microscopy.

RESULTS: Rats with DMH-induced colon adenocarcinomas showed elevated transepithelial electrical resistance in the areas of neoplasm development. In contrast, there was no change in the electrophysiological properties of tumor adjacent areas, however, the paracellular permeability of these areas to fluorescein was increased compared to the control rats and was characterized by sharply reduced barrier function.

CONCLUSIONS: The barrier properties of the colon vary depending on tumor location. The tumors were less permeable than the intact intestinal wall and probably have a negative influence on tumor-adjacent tissues by disrupting their barrier function.

Keywords: 1,2-dimethylhydrazine; Rat; Ussing chamber; colorectal cancer; intestinal permeability; short circuit current; transepithelial electrical resistance

References

  1. Int J Cancer. 2015 Mar 1;136(5):E359-86 - PubMed
  2. Int J Cancer. 1975 Apr 15;15(4):673-83 - PubMed
  3. Ann N Y Acad Sci. 2000;915:231-6 - PubMed
  4. Crit Care Med. 1999 Aug;27(8):1429-36 - PubMed
  5. Shock. 1997 Aug;8(2):108-14 - PubMed
  6. Cytokine. 2012 Aug;59(2):264-72 - PubMed
  7. Gastroenterology. 1987 Sep;93(3):449-55 - PubMed
  8. Int J Mol Med. 2009 Jan;23(1):41-8 - PubMed
  9. J Natl Cancer Inst. 1975 May;54(5):1115-35 - PubMed
  10. Gut. 1986 Sep;27(9):999-1005 - PubMed
  11. Cancer Lett. 1977 Mar;2(4-5):185-90 - PubMed
  12. Carcinogenesis. 1999 Aug;20(8):1425-31 - PubMed
  13. Am J Physiol. 1997 May;272(5 Pt 1):G923-34 - PubMed
  14. Acta Physiol (Oxf). 2016 Jan;216(1):112-9 - PubMed
  15. Cancer Epidemiol Biomarkers Prev. 2015 Feb;24(2):406-14 - PubMed
  16. Am J Physiol. 1975 Jan;228(1):191-5 - PubMed
  17. J Biochem Biophys Methods. 1998 Sep 24;37(1-2):35-46 - PubMed
  18. Curr Opin Crit Care. 2003 Apr;9(2):143-51 - PubMed
  19. Front Biosci (Landmark Ed). 2009 Jan 01;14:2765-78 - PubMed
  20. Gastroenterol Clin North Am. 1998 Jun;27(2):289-307 - PubMed
  21. PLoS One. 2017 Feb 2;12(2):e0171045 - PubMed
  22. Rev Esp Enferm Dig. 2007 Aug;99(8):463-6 - PubMed
  23. Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1976 Sep 24;87(1):67-80 - PubMed
  24. Pflugers Arch. 1997 Jan;433(3):254-9 - PubMed
  25. Am J Physiol Gastrointest Liver Physiol. 2011 Jun;300(6):G1054-64 - PubMed
  26. ScientificWorldJournal. 2011 Apr 05;11:826-41 - PubMed
  27. Front Immunol. 2013 Sep 17;4:280 - PubMed
  28. Gut. 1993 May;34(5):616-20 - PubMed
  29. Lab Anim. 2014 Feb 4;48(3):178-192 - PubMed
  30. Physiol Res. 2016 Dec 13;65(6):1053-1058 - PubMed
  31. Gastroenterology. 1990 Jun;98(6):1502-8 - PubMed
  32. J Cell Biol. 1996 Aug;134(4):1031-49 - PubMed
  33. World J Gastroenterol. 2016 Mar 21;22(11):3117-26 - PubMed
  34. Int J Pharm. 2004 Mar 19;272(1-2):173-80 - PubMed
  35. Dig Dis Sci. 2016 Sep;61(9):2522-34 - PubMed
  36. J Biomed Biotechnol. 2011;2011:473964 - PubMed
  37. Crit Care Med. 1999 Oct;27(10):2246-51 - PubMed
  38. Gastroenterol Res Pract. 2016;2016:7374197 - PubMed
  39. J Comp Physiol B. 2010 Apr;180(4):591-8 - PubMed
  40. J Pharmacol Exp Ther. 2002 Oct;303(1):17-28 - PubMed
  41. Drug Discov Today. 2005 Mar 15;10(6):395-408 - PubMed
  42. Int J Colorectal Dis. 2007 Jun;22(6):651-9 - PubMed
  43. Pflugers Arch. 1997 Nov;434(6):801-8 - PubMed
  44. Physiol Rev. 2002 Jan;82(1):245-89 - PubMed
  45. Gastroenterology. 2013 Apr;144(4):705-17 - PubMed
  46. PLoS One. 2013 Jul 09;8(7):e68162 - PubMed
  47. Immunity. 2015 Oct 20;43(4):727-38 - PubMed
  48. Histol Histopathol. 2001 Oct;16(4):1183-95 - PubMed
  49. Ross Fiziol Zh Im I M Sechenova. 2011 Oct;97(10):1066-83 - PubMed
  50. Semin Cell Dev Biol. 2014 Dec;36:166-76 - PubMed
  51. PLoS One. 2014 Jun 19;9(6):e100621 - PubMed
  52. Oncol Res. 2001;12(11-12):469-76 - PubMed
  53. Cancer Biol Med. 2017 Feb;14(1):100-107 - PubMed
  54. Am J Physiol. 1994 Nov;267(5 Pt 1):G892-900 - PubMed
  55. Am J Pathol. 1997 Jul;151(1):45-54 - PubMed
  56. J Oncol. 2010;2010:541957 - PubMed
  57. Biomed Res Int. 2013;2013:725710 - PubMed
  58. Mol Biol Cell. 2009 Aug;20(16):3713-24 - PubMed
  59. Am J Physiol Gastrointest Liver Physiol. 2000 Nov;279(5):G851-7 - PubMed

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