Display options
Cite
Tarahomi M, de Melker AA, van Wely M, et al. pH stability of human preimplantation embryo culture media: effects of culture and batches. Reprod Biomed Online. 2018;37(4):409-414doi: 10.1016/j.rbmo.2018.08.011.
Tarahomi, M., de Melker, A. A., van Wely, M., Hamer, G., Repping, S., & Mastenbroek, S. (2018). pH stability of human preimplantation embryo culture media: effects of culture and batches. Reproductive biomedicine online, 37(4), 409-414. https://doi.org/10.1016/j.rbmo.2018.08.011
Tarahomi, Majid, et al. "pH stability of human preimplantation embryo culture media: effects of culture and batches." Reproductive biomedicine online vol. 37,4 (2018): 409-414. doi: https://doi.org/10.1016/j.rbmo.2018.08.011
Tarahomi M, de Melker AA, van Wely M, Hamer G, Repping S, Mastenbroek S. pH stability of human preimplantation embryo culture media: effects of culture and batches. Reprod Biomed Online. 2018 Oct;37(4):409-414. doi: 10.1016/j.rbmo.2018.08.011. Epub 2018 Aug 29. PMID: 30228072.
Copy Download .nbib Format: NLM
Share it on

Reprod Biomed Online. 2018 Oct;37(4):409-414. doi: 10.1016/j.rbmo.2018.08.011. Epub 2018 Aug 29.

pH stability of human preimplantation embryo culture media: effects of culture and batches.

Reproductive biomedicine online

Majid Tarahomi, Annemieke A de Melker, Madelon van Wely, Geert Hamer, Sjoerd Repping, Sebastiaan Mastenbroek

Affiliations

  1. Centre for Reproductive Medicine, Amsterdam Reproduction and Development Research Institute, Academic Medical Centre, University of AmsterdamAmsterdam, the Netherlands; Reproductive Biotechnology Research Centre, Avicenna Research Institute, ACECRTehran, Iran.
  2. Centre for Reproductive Medicine, Amsterdam Reproduction and Development Research Institute, Academic Medical Centre, University of AmsterdamAmsterdam, the Netherlands.
  3. Centre for Reproductive Medicine, Amsterdam Reproduction and Development Research Institute, Academic Medical Centre, University of AmsterdamAmsterdam, the Netherlands. Electronic address: [email protected].

PMID: 30228072 DOI: 10.1016/j.rbmo.2018.08.011

Abstract

RESEARCH QUESTION: How stable is the pH of human preimplantation embryo culture media during IVF culture and is there variation in pH between batches of culture media?

DESIGN: To evaluate pH stability, three batches of three culture media were incubated in triplicate without embryos (sham culture) at CO

RESULTS: An increase in pH during 4 days of culture was found in all three culture media, but the observed increased values were within the generally accepted range for clinical practice (pH 7.2-7.4). One medium was pH 7.1 in the first 2 days, but this was within the range provided by the manufacturer for that medium. Three out of five analysed media showed batch variation in pH that exceeded the generally accepted range for clinical practice.

CONCLUSIONS: A relevant difference in pH was found between batches of human preimplantation embryo culture media. This suggests that the CO

Copyright © 2018. Published by Elsevier Ltd.

Keywords: Culture medium; Human preimplantation embryo culture; IVF; Intracytoplasmic sperm injection; pH

Substances

MeSH terms

Publication Types