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Anderson M, Choga WT, Moyo S, et al. Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana. Genes (Basel). 2018;9(9)doi: 10.3390/genes9090453.
Anderson, M., Choga, W. T., Moyo, S., Bell, T. G., Mbangiwa, T., Phinius, B. B., Bhebhe, L., Sebunya, T. K., Lockman, S., Marlink, R., Kramvis, A., Essex, M., Musonda, R. M., Blackard, J. T., & Gaseitsiwe, S. (2018). Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana. Genes, 9(9), . https://doi.org/10.3390/genes9090453
Anderson, Motswedi, et al. "Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana." Genes vol. 9,9 (2018). doi: https://doi.org/10.3390/genes9090453
Anderson M, Choga WT, Moyo S, Bell TG, Mbangiwa T, Phinius BB, Bhebhe L, Sebunya TK, Lockman S, Marlink R, Kramvis A, Essex M, Musonda RM, Blackard JT, Gaseitsiwe S. Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana. Genes (Basel). 2018 Sep 07;9(9). doi: 10.3390/genes9090453. PMID: 30205537; PMCID: PMC6162474.
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Genes (Basel). 2018 Sep 07;9(9). doi: 10.3390/genes9090453.

Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana.

Genes

Motswedi Anderson, Wonderful Tatenda Choga, Sikhulile Moyo, Trevor Graham Bell, Tshepiso Mbangiwa, Bonolo Bonita Phinius, Lynnette Bhebhe, Theresa Kibirige Sebunya, Shahin Lockman, Richard Marlink, Anna Kramvis, Max Essex, Rosemary Mubanga Musonda, Jason Tory Blackard, Simani Gaseitsiwe

Affiliations

  1. Botswana Harvard AIDS Institute Partnership, Gaborone, Botswana. [email protected].
  2. Department of Biological Sciences, Faculty of Science, University of Botswana, Gaborone, Botswana. [email protected].
  3. Botswana Harvard AIDS Institute Partnership, Gaborone, Botswana. [email protected].
  4. Botswana Harvard AIDS Institute Partnership, Gaborone, Botswana. [email protected].
  5. Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA 02138, USA. [email protected].
  6. Hepatitis Virus Diversity Research Unit (HVDRU), Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2050, South Africa. [email protected].
  7. Botswana Harvard AIDS Institute Partnership, Gaborone, Botswana. [email protected].
  8. Faculty of Allied Health Sciences, University of Botswana, Gaborone, Botswana. [email protected].
  9. Botswana Harvard AIDS Institute Partnership, Gaborone, Botswana. [email protected].
  10. Botswana Harvard AIDS Institute Partnership, Gaborone, Botswana. [email protected].
  11. Department of Biological Sciences, Faculty of Science, University of Botswana, Gaborone, Botswana. [email protected].
  12. Botswana Harvard AIDS Institute Partnership, Gaborone, Botswana. [email protected].
  13. Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA 02138, USA. [email protected].
  14. Botswana Harvard AIDS Institute Partnership, Gaborone, Botswana. [email protected].
  15. Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA 02138, USA. [email protected].
  16. Rutgers Global Health Institute, Robert Wood Johnson Medical School, Rutgers University, New Brunswick, NJ 08901, USA. [email protected].
  17. Hepatitis Virus Diversity Research Unit (HVDRU), Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2050, South Africa. [email protected].
  18. Botswana Harvard AIDS Institute Partnership, Gaborone, Botswana. [email protected].
  19. Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA 02138, USA. [email protected].
  20. Botswana Harvard AIDS Institute Partnership, Gaborone, Botswana. [email protected].
  21. College of Medicine, University of Cincinnati, Cincinnati, OH 45627, USA. [email protected].
  22. Botswana Harvard AIDS Institute Partnership, Gaborone, Botswana. [email protected].
  23. Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA 02138, USA. [email protected].

PMID: 30205537 PMCID: PMC6162474 DOI: 10.3390/genes9090453

Abstract

The World Health Organization plans to eliminate hepatitis B and C Infections by 2030. Therefore, there is a need to study and understand hepatitis B virus (HBV) epidemiology and viral evolution further, including evaluating occult (HBsAg-negative) HBV infection (OBI), given that such infections are frequently undiagnosed and rarely treated. We aimed to molecularly characterize HBV genomes from 108 individuals co-infected with human immunodeficiency virus (HIV) and chronic hepatitis B (CHB) or OBI identified from previous HIV studies conducted in Botswana from 2009 to 2012. Full-length (3.2 kb) and nearly full-length (~3 kb) genomes were amplified by nested polymerase chain reaction (PCR). Sequences from OBI participants were compared to sequences from CHB participants and GenBank references to identify OBI-unique mutations. HBV genomes from 50 (25 CHB and 25 OBI) individuals were successfully genotyped. Among OBI participants, subgenotype A1 was identified in 12 (48%), D3 in 12 (48%), and E in 1 (4%). A similar genotype distribution was observed in CHB participants. Whole HBV genome sequences from Botswana, representing OBI and CHB, were compared for the first time. There were 43 OBI-unique mutations, of which 26 were novel. Future studies using larger sample sizes and functional analysis of OBI-unique mutations are warranted.

Keywords: Africa; Botswana; HBV; chronic hepatitis B; mutations; occult hepatitis B

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