J Cell Biochem. 2018 Sep 11; doi: 10.1002/jcb.27492. Epub 2018 Sep 11.
Comparison of large-scale deletions of the sperm mitochondrial DNA in normozoospermic and asthenoteratozoospermic men.
Journal of cellular biochemistry
Maryam Gholinezhad, Yousefreza Yousefnia-Pasha, Abasalt Hosseinzadeh Colagar, Milad Mohammadoo-Khorasani, Ali Bidmeshkipour
Affiliations
Affiliations
- Department of Biology, Faculty of Basic Sciences, Razi University, Kermanshah, Iran.
- Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
- Departement of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran.
- Department of Clinical Biochemistry, Faculty of Medical Sciences,Tarbiat Modares University, Tehran, Iran.
PMID: 30206972
DOI: 10.1002/jcb.27492
Abstract
BACKGROUND AND OBJECTIVE: Mitochondria play a crucial role in energy metabolism for the survival and motility of sperm during fertilization. The aim of this study was to determine the association of large-scale mitochondrial DNA deletions with abnormal sperm motility and morphology in asthenoteratozoospermic patients.
MATERIALS AND METHODS: In this case-control study, 41 semen samples were collected from 18 normozoospermic healthy men and 23 asthenoteratozoospermic patients, according to the WHO guidelines. The swim-up technique was used for separation of spermatozoa on the basis of their motility. Long-range polymerase chain reaction (PCR) was used for screening of mitochondrial DNA (mtDNA) large-scale deletions, and primer shift PCR was used for confirmation of deletions.
RESULTS: The mean sperm motility, normal morphology, and progressive motility in asthenoteratozoospermic patients were significantly lower than in the normozoospermic group (P < 0.0001). There was a positive significant correlation between motility and normal sperm morphology ( P < 0.0001, r = 0.741). The results of long-range PCR revealed the existence of 4866-bp deletion along with the two common 4977-bp and 7436-bp deleted mtDNA in both groups. However, the frequency of multiple mtDNA deletions in the asthenoteratozoospermic group (15/23, 65.22%) was significantly higher than that in the normozoospermic group (7/18, 38.89%). Direct sequencing of the 534-bp PCR product revealed that it was amplified from the mtDNA with a 4866-bp deletion flanked by a seven-nucleotide direct repeat (5'-ACCCCCT-3').
CONCLUSIONS: Our findings suggested that these large-scale deletions of mtDNA may be genetic risk factors for poor sperm quality in asthenoteratozoospermia-induced male infertility. Thus, it is necessary to understand the mechanisms behind the generation of these deletions.
© 2018 Wiley Periodicals, Inc.
Keywords: asthenoteratozoospermia; large-scale deletions; mitochondrial DNA (mtDNA); sperm quality
Publication Types
Grant support