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Long L, Xie X, Tang Y. Distribution of Tryptophan Hydroxylase Immunoreactivity in the Spiral Ganglion Neurons of Mouse Cochlea. Indian J Otolaryngol Head Neck Surg. 2018;70(3):425-427doi: 10.1007/s12070-018-1448-7.
Long, L., Xie, X., & Tang, Y. (2018). Distribution of Tryptophan Hydroxylase Immunoreactivity in the Spiral Ganglion Neurons of Mouse Cochlea. Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India, 70(3), 425-427. https://doi.org/10.1007/s12070-018-1448-7
Long, Lili, et al. "Distribution of Tryptophan Hydroxylase Immunoreactivity in the Spiral Ganglion Neurons of Mouse Cochlea." Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India vol. 70,3 (2018): 425-427. doi: https://doi.org/10.1007/s12070-018-1448-7
Long L, Xie X, Tang Y. Distribution of Tryptophan Hydroxylase Immunoreactivity in the Spiral Ganglion Neurons of Mouse Cochlea. Indian J Otolaryngol Head Neck Surg. 2018 Sep;70(3):425-427. doi: 10.1007/s12070-018-1448-7. Epub 2018 Jul 09. PMID: 30211102; PMCID: PMC6127042.
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Indian J Otolaryngol Head Neck Surg. 2018 Sep;70(3):425-427. doi: 10.1007/s12070-018-1448-7. Epub 2018 Jul 09.

Distribution of Tryptophan Hydroxylase Immunoreactivity in the Spiral Ganglion Neurons of Mouse Cochlea.

Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India

Lili Long, Xiaoyan Xie, Yuedi Tang

Affiliations

  1. 1Department of Otorhinolaryngology-Head and Neck Surgery, West China Hospital of Sichuan University, No. 37 Guo-Xue-Xiang, Chengdu, 610041 Sichuan China.
  2. 5Present Address: Department of Otorhinolaryngology, Sichuan University Hospital of Sichuan University, Chengdu, Sichuan China.
  3. 2West China Institute of Women and Children's Health, National Center for Birth Defect Monitoring, West China Second Hospital of Sichuan University, Chengdu, Sichuan China.
  4. 3Department of Obstetric and Gynecologic, West China Second University Hospital, Sichuan University, Chengdu, Sichuan China.
  5. Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, No. 17, Section 3, Renmin South Road, Chengdu, 610041 Sichuan China.

PMID: 30211102 PMCID: PMC6127042 DOI: 10.1007/s12070-018-1448-7

Abstract

Serotonin, also known as 5-hydroxytryptamine, is an important neurotransmitter and neuromodulator in the nervous system. Spiral ganglion neurons (SGNs) are the first station of the peripheral afferent auditory nervous system. We have previously demonstrated positive serotonin immunoreactivity in mouse SGNs. Whether serotonin in the SGNs is absorbed from the circumstances, or is synthesized by the neurons themselves is unknown. The purpose of this study was to find out the source of serotonin in the SGNs. Tryptophan hydroxylase is the rate-limiting enzyme in the process of serotonin biosynthesis. We detected tryptophan hydroxylase in the mouse SGNs using immunohistochemistry. The positive immunostaining of tryptophan hydroxylase was observed in the cytoplasm of SGNs. The cytoplasm of the SGNs was not get stained in the negative control group. Our results suggest that SGNs can synthesize serotonin themselves, and they may serve as an additional source of serotonin in the cochlea. The SGNs are serotonergic neurons. The receptors of serotonin on the membrane of SGNs interact with serotonin as auto-receptors. Serotonin in these neurons plays its physiological role in an autocrine/paracrine way.

Keywords: Immunohistochemistry; Serotonin; Spiral ganglion neuron; Tryptophan hydroxylase

Conflict of interest statement

The authors declare that they have no conflict of interest.All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures perform

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