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Kolettis TM, Bagli E, Barka E, et al. Medium-term Electrophysiologic Effects of a Cellularized Scaffold Implanted in Rats After Myocardial Infarction. Cureus. 2018;10(7):e2959doi: 10.7759/cureus.2959.
Kolettis, T. M., Bagli, E., Barka, E., Kouroupis, D., Kontonika, M., Vilaeti, A. D., Markou, M., Roumpi, M., Maltabe, V., La Rocca, V., Agathopoulos, S., & Fotsis, T. (2018). Medium-term Electrophysiologic Effects of a Cellularized Scaffold Implanted in Rats After Myocardial Infarction. Cureus, 10(7), e2959. https://doi.org/10.7759/cureus.2959
Kolettis, Theofilos M, et al. "Medium-term Electrophysiologic Effects of a Cellularized Scaffold Implanted in Rats After Myocardial Infarction." Cureus vol. 10,7 (2018): e2959. doi: https://doi.org/10.7759/cureus.2959
Kolettis TM, Bagli E, Barka E, Kouroupis D, Kontonika M, Vilaeti AD, Markou M, Roumpi M, Maltabe V, La Rocca V, Agathopoulos S, Fotsis T. Medium-term Electrophysiologic Effects of a Cellularized Scaffold Implanted in Rats After Myocardial Infarction. Cureus. 2018 Jul 10;10(7):e2959. doi: 10.7759/cureus.2959. PMID: 30214847; PMCID: PMC6132679.
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Cureus. 2018 Jul 10;10(7):e2959. doi: 10.7759/cureus.2959.

Medium-term Electrophysiologic Effects of a Cellularized Scaffold Implanted in Rats After Myocardial Infarction.

Cureus

Theofilos M Kolettis, Eleni Bagli, Eleonora Barka, Dimitrios Kouroupis, Marianthi Kontonika, Agapi D Vilaeti, Maria Markou, Maria Roumpi, Violetta Maltabe, Vassilios La Rocca, Simeon Agathopoulos, Theodore Fotsis

Affiliations

  1. Cardiology, Cardiovascular Research Institute, Ioannina, GRC.
  2. Division of Biomedical Research, Institute of Molecular Biology and Biotechnology - Forth, Ioannina, GRC.
  3. Ceramics and Composites Laboratory, Materials Science and Engineering, University of Ioannina, Ioannina, GRC.
  4. Cardiology, Cardiovascular Research Institute, Athens, GRC.
  5. Ceramics and Composites Laboratory, Science and Engineering, University of Ioannina, Ioannina, GRC.

PMID: 30214847 PMCID: PMC6132679 DOI: 10.7759/cureus.2959

Abstract

Background Cardiac repair strategies are being evaluated for myocardial infarctions, but the safety issues regarding their arrhythmogenic potential remain unresolved. By utilizing the in-vivo rat model, we have examined the medium-term electrophysiologic effects of a biomaterial scaffold that has been cellularized with spheroids of human adipose tissue, derived from mesenchymal stem cells and umbilical vein endothelial cells. Methods Mesenchymal stem cells, which exhibit adequate differentiation capacity, were co-cultured with umbilical vein endothelial cells and were seeded on an alginate based scaffold. After in-vitro characterization, the cellularized scaffold was implanted in (n=15) adult Wistar rats 15 min post ligation of the left coronary artery, with an equal number of animals serving as controls. Two weeks thereafter, monophasic action potentials were recorded and activation-mapping was performed with a multi-electrode array. An arrhythmia score for inducible ventricular tachyarrhythmias was calculated after programmed electrical stimulation. Results The arrhythmia score was comparable between the treated animals and controls. No differences were detected in the local conduction at the infarct border and in the voltage rise in monophasic action potential recordings. Treatment did not affect the duration of local repolarization, but tended to enhance its dispersion. Conclusions The fabricated bi-culture cellularized scaffold displayed favorable properties after in-vitro characterization. Medium-term electrophysiologic assessment after implantation in the infarcted rat myocardium revealed low arrhythmogenic potential, but the long-term effects on repolarization dispersion will require further investigation.

Keywords: alginate-scaffold; cardiac repair; conduction-delay; mesenchymal stem-cells; myocardial infarction; repolarization-dispersion; ventricular arrhythmias

Conflict of interest statement

The authors have declared that no competing interests exist.

References

  1. Acta Biomater. 2015 Oct;26:13-22 - PubMed
  2. Cardiovasc Res. 2003 May 1;58(2):351-7 - PubMed
  3. Physiol Rev. 2004 Apr;84(2):431-88 - PubMed
  4. Int J Cardiol. 2006 Aug 10;111(2):231-9 - PubMed
  5. J Cardiovasc Electrophysiol. 2013 Jul;24(7):813-21 - PubMed
  6. J Mol Cell Cardiol. 2007 Jun;42(6):1016-25 - PubMed
  7. Circulation. 1998 Nov 10;98(19):2074-80 - PubMed
  8. Circ Res. 2005 Jul 22;97(2):159-67 - PubMed
  9. Life Sci. 2016 Jan 1;144:170-7 - PubMed
  10. Curr Opin Pharmacol. 2013 Apr;13(2):210-7 - PubMed
  11. Tissue Eng Part A. 2010 Jan;16(1):115-25 - PubMed
  12. Mini Rev Med Chem. 2011 Mar;11(3):263-70 - PubMed
  13. J Mol Cell Cardiol. 2007 Feb;42(2):304-14 - PubMed
  14. J Lab Clin Med. 2000 Apr;135(4):316-23 - PubMed
  15. Stem Cell Rev. 2013 Jun;9(3):339-49 - PubMed
  16. Cytotherapy. 2006;8(4):315-7 - PubMed
  17. Europace. 2007 Dec;9(12):1218-21 - PubMed
  18. Curr Pharm Des. 2014;20(12):1925-9 - PubMed
  19. Int J Cardiol. 2011 Nov 3;152(3):314-20 - PubMed
  20. Lancet. 2004 Jul 10-16;364(9429):141-8 - PubMed
  21. Int J Cardiol. 2013 May 10;165(2):278-84 - PubMed
  22. Circulation. 2006 Apr 18;113(15):1832-41 - PubMed
  23. Circ Res. 1997 Jul;81(1):110-9 - PubMed
  24. Cardiovasc Res. 2006 Feb 1;69(2):299-301 - PubMed
  25. Biomaterials. 2010 Sep;31(27):7012-20 - PubMed
  26. Circulation. 2015 Aug 25;132(8):772-84 - PubMed
  27. Cardiovasc Res. 2006 Feb 1;69(2):348-58 - PubMed
  28. Growth Factors. 2015;33(4):250-8 - PubMed
  29. Circulation. 2004 Feb 10;109(5):656-63 - PubMed

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