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Richter FG, Fendl S, Haag J, et al. Glutamate Signaling in the Fly Visual System. iScience. 2018;7:85-95doi: 10.1016/j.isci.2018.08.019.
Richter, F. G., Fendl, S., Haag, J., Drews, M. S., & Borst, A. (2018). Glutamate Signaling in the Fly Visual System. iScience, 785-95. https://doi.org/10.1016/j.isci.2018.08.019
Richter, Florian G, et al. "Glutamate Signaling in the Fly Visual System." iScience vol. 7 (2018): 85-95. doi: https://doi.org/10.1016/j.isci.2018.08.019
Richter FG, Fendl S, Haag J, Drews MS, Borst A. Glutamate Signaling in the Fly Visual System. iScience. 2018 Sep 28;7:85-95. doi: 10.1016/j.isci.2018.08.019. Epub 2018 Aug 29. PMID: 30267688; PMCID: PMC6135900.
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iScience. 2018 Sep 28;7:85-95. doi: 10.1016/j.isci.2018.08.019. Epub 2018 Aug 29.

Glutamate Signaling in the Fly Visual System.

iScience

Florian G Richter, Sandra Fendl, Jürgen Haag, Michael S Drews, Alexander Borst

Affiliations

  1. Max-Planck-Institute of Neurobiology, 82152 Martinsried, Germany. Electronic address: [email protected].
  2. Max-Planck-Institute of Neurobiology, 82152 Martinsried, Germany.
  3. Max-Planck-Institute of Neurobiology, 82152 Martinsried, Germany. Electronic address: [email protected].

PMID: 30267688 PMCID: PMC6135900 DOI: 10.1016/j.isci.2018.08.019

Abstract

For a proper understanding of neural circuit function, it is important to know which signals neurons relay to their downstream partners. Calcium imaging with genetically encoded calcium sensors like GCaMP has become the default approach for mapping these responses. How well such measurements represent the true neurotransmitter output of any given cell, however, remains unclear. Here, we demonstrate the viability of the glutamate sensor iGluSnFR for 2-photon in vivo imaging in Drosophila melanogaster and prove its usefulness for estimating spatiotemporal receptive fields in the visual system. We compare the results obtained with iGluSnFR with the ones obtained with GCaMP6f and find that the spatial aspects of the receptive fields are preserved between indicators. In the temporal domain, however, measurements obtained with iGluSnFR reveal the underlying response properties to be much faster than those acquired with GCaMP6f. Our approach thus offers a more accurate description of glutamatergic neurons in the fruit fly.

Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

Keywords: Optical Imaging; Sensory Neuroscience; Techniques in Neuroscience

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