Display options
Cite
Kaleka CC, Zucconi E, Vieira TDS, et al. Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells. Rev Bras Ortop. 2018;53(5):557-563doi: 10.1016/j.rboe.2018.07.009.
Kaleka, C. C., Zucconi, E., Vieira, T. D. S., Secco, M., Ferretti, M., & Cohen, M. (2018). Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells. Revista brasileira de ortopedia, 53(5), 557-563. https://doi.org/10.1016/j.rboe.2018.07.009
Kaleka, Camila Cohen, et al. "Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells." Revista brasileira de ortopedia vol. 53,5 (2018): 557-563. doi: https://doi.org/10.1016/j.rboe.2018.07.009
Kaleka CC, Zucconi E, Vieira TDS, Secco M, Ferretti M, Cohen M. Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells. Rev Bras Ortop. 2018 Aug 02;53(5):557-563. doi: 10.1016/j.rboe.2018.07.009. eCollection 2018. PMID: 30245994; PMCID: PMC6147760.
Copy Download .nbib Format: NLM
Share it on

Rev Bras Ortop. 2018 Aug 02;53(5):557-563. doi: 10.1016/j.rboe.2018.07.009. eCollection 2018.

Evaluation of different commercial hyaluronic acids as a vehicle for injection of human adipose-derived mesenchymal stem cells.

Revista brasileira de ortopedia

Camila Cohen Kaleka, Eder Zucconi, Tierri da Silva Vieira, Mariane Secco, Mário Ferretti, Moisés Cohen

Affiliations

  1. Instituto Cohen Ortopedia, Reabilitação e Medicina do Esporte, São Paulo, SP, Brazil.
  2. Programa do Aparelho Locomotor, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
  3. Departamento de Genética e Biologia Evolutiva, Universidade de São Paulo (USP), São Paulo, SP, Brazil.
  4. Laboratório StemCorp de Tecnologia em Células-Tronco, São Paulo, SP, Brazil.

PMID: 30245994 PMCID: PMC6147760 DOI: 10.1016/j.rboe.2018.07.009

Abstract

OBJECTIVE: The main purpose of this study is to evaluate,

METHODS: AD-MSCs were divided into seven groups: one control group where AD-MSCs were cultivated with phosphate-buffered saline (PBS) and six other groups where AD-MSCs were cultivated with different commercial brands of hyaluronic acid. AD-MSC viability analysis was performed after 4, 24, and 48 h in contact with each treatment, using the trypan staining method on a Countess automated cell counter (Thermo Fisher Scientific).

RESULTS: The results clearly demonstrated a significant difference in cell viability when AD-MSCs were exposed to different hyaluronic acids when compared with the control group.

CONCLUSION: These data suggest that hyaluronic acid can be used as a vehicle for injection of human AD-MSCs, but caution is needed to choose the best product, aiming at its future therapeutic application.

Keywords: Arthroscopy; Articular cartilage; Cartilage diseases; Knee; Mesenchymal stem cell transplantation; Mesenchymal stem cells

References

  1. Sci China C Life Sci. 2007 Aug;50(4):429-37 - PubMed
  2. Stem Cells Int. 2012;2012:394962 - PubMed
  3. Plast Reconstr Surg. 2008 Apr;121(4):1153-64 - PubMed
  4. Front Biosci (Schol Ed). 2011 Jan 01;3:69-81 - PubMed
  5. Stem Cells. 2008 Jan;26(1):146-50 - PubMed
  6. J Craniomaxillofac Surg. 2014 Sep;42(6):825-34 - PubMed
  7. Mol Pharm. 2011 Oct 3;8(5):1582-91 - PubMed
  8. J Clin Orthop Trauma. 2016 Jul-Sep;7(3):157-63 - PubMed
  9. Musculoskelet Surg. 2017 Apr;101(1):45-49 - PubMed
  10. Tissue Eng Part C Methods. 2009 Dec;15(4):595-604 - PubMed
  11. N Engl J Med. 1994 Oct 6;331(14):889-95 - PubMed
  12. Semin Arthritis Rheum. 2016 Feb;45(4 Suppl):S28-33 - PubMed
  13. J Orthop Res. 2009 May;27(5):612-9 - PubMed
  14. Stem Cells. 2008 Sep;26(9):2391-8 - PubMed
  15. Langenbecks Arch Surg. 2009 Nov;394(6):985-97 - PubMed
  16. Cytotherapy. 2006;8(4):315-7 - PubMed
  17. Knee Surg Sports Traumatol Arthrosc. 2013 Aug;21(8):1717-29 - PubMed
  18. Acta Biomater. 2014 Apr;10(4):1571-1580 - PubMed
  19. Stem Cell Rev. 2010 Dec;6(4):560-6 - PubMed
  20. Clin Med Insights Arthritis Musculoskelet Disord. 2016 Sep 25;9:175-9 - PubMed
  21. Cell Biochem Biophys. 2008;52(2):85-102 - PubMed
  22. In Vitro Cell Dev Biol Anim. 2007 Feb;43(2):49-58 - PubMed
  23. Stem Cell Res Ther. 2010 Jun 29;1(2):19 - PubMed
  24. Biol Cell. 2008 Apr;100(4):231-41 - PubMed

Publication Types