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Hage CH, Gomes JT, Bardet SM, et al. Two-photon microscopy with a frequency-doubled fully fusion-spliced fiber laser at 1840  nm. Opt Lett. 2018;43(20):5098-5101doi: 10.1364/OL.43.005098.
Hage, C. H., Gomes, J. T., Bardet, S. M., Granger, G., Jossent, M., Lavoute, L., Gaponov, D., & Fevrier, S. (2018). Two-photon microscopy with a frequency-doubled fully fusion-spliced fiber laser at 1840  nm. Optics letters, 43(20), 5098-5101. https://doi.org/10.1364/OL.43.005098
Hage, C-H, et al. "Two-photon microscopy with a frequency-doubled fully fusion-spliced fiber laser at 1840  nm." Optics letters vol. 43,20 (2018): 5098-5101. doi: https://doi.org/10.1364/OL.43.005098
Hage CH, Gomes JT, Bardet SM, Granger G, Jossent M, Lavoute L, Gaponov D, Fevrier S. Two-photon microscopy with a frequency-doubled fully fusion-spliced fiber laser at 1840  nm. Opt Lett. 2018 Oct 15;43(20):5098-5101. doi: 10.1364/OL.43.005098. PMID: 30320829.
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Opt Lett. 2018 Oct 15;43(20):5098-5101. doi: 10.1364/OL.43.005098.

Two-photon microscopy with a frequency-doubled fully fusion-spliced fiber laser at 1840  nm.

Optics letters

C-H Hage, J-T Gomes, S M Bardet, G Granger, M Jossent, L Lavoute, D Gaponov, S Fevrier

PMID: 30320829 DOI: 10.1364/OL.43.005098

Abstract

We introduce a fiber-based laser system providing 130 fs pulses with 3.5 nJ energy at 920 nm at a 43 MHz repetition rate and illustrate the potential of the source for two-photon excited fluorescence microscopy of living mouse brain. The laser source is based on frequency-doubling high-energy solitons generated and frequency-shifted to 1840 nm in large mode area fibers. This simple laser system could unleash the potential of two-photon microscopy techniques in the biology laboratory where green fluorescent proteins with two-photon absorption spectrum peaking around 920 nm are routinely used.

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