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Seidman SA, Johnson NA, Arbelo U, et al. Tissue protein and lipid alterations in response to metallic impaction. J Cell Biochem. 2018;doi: 10.1002/jcb.27563.
Seidman, S. A., Johnson, N. A., Arbelo, U., Aribindi, K., & Bhattacharya, S. K. (2018). Tissue protein and lipid alterations in response to metallic impaction. Journal of cellular biochemistry, . https://doi.org/10.1002/jcb.27563
Seidman, Shari A, et al. "Tissue protein and lipid alterations in response to metallic impaction." Journal of cellular biochemistry vol. (2018). doi: https://doi.org/10.1002/jcb.27563
Seidman SA, Johnson NA, Arbelo U, Aribindi K, Bhattacharya SK. Tissue protein and lipid alterations in response to metallic impaction. J Cell Biochem. 2018 Sep 11; doi: 10.1002/jcb.27563. Epub 2018 Sep 11. PMID: 30277616.
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J Cell Biochem. 2018 Sep 11; doi: 10.1002/jcb.27563. Epub 2018 Sep 11.

Tissue protein and lipid alterations in response to metallic impaction.

Journal of cellular biochemistry

Shari A Seidman, Natasha A Johnson, Ulises Arbelo, Katyayini Aribindi, Sanjoy K Bhattacharya

Affiliations

  1. Bascom Palmer Eye Institute, McKnight Research Center, University of Miami, Miami, Florida.

PMID: 30277616 DOI: 10.1002/jcb.27563

Abstract

Tissue response to metallic contact and injury remains poorly understood at the level of proteins and lipid changes. We used corneas as a model system (bovine, porcine [n = 300 each], and human corneas [n = 6]) to characterize proteomic and lipidomic reactions to metallic exposure (impaction with iron). We also made a limited investigation into protein extractability and profile changes due to copper, and lead exposure as well. We identified selected proteins after trypsin digestion using an LCQ Deca XP and lipids using a TSQ Quantum Access Max mass spectrometer, respectively. Our findings indicate that iron impaction to corneal tissue results in cleavage of 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-2 variant (PLCB2; 134 kDa) into a 36 kDa species and presence of the epithelial layer is necessary for this cleavage. Penetration of the cornea with other metals (copper and lead) resulted in lower protein extractability from corneal tissue compared with controls but not distinct changes in PLCB2. The changes in protein profiles were unique to the type of metal used for impaction. The depth of injury negatively affected protein extractability and profile compared with controls. These changes were also dependent on several other factors in a complex manner. Iron impaction of corneal tissue for 24 hours results in cleavage of PLCB2 commensurate with significant changes in phosphatidylinositols but not phosphatidylcholines or other phospholipids.

© 2018 Wiley Periodicals, Inc.

Keywords: HPLC; cell biology; cornea; immunohistochemistry; iron; mass spectrometry; metallic impaction; protein identification; protein sequence

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