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Brzezicka KA, Serna S, Reichardt NC. Fluorescent Neoglycoprotein Gold Nanoclusters: Synthesis and Applications in Plant Lectin Sensing and Cell Imaging. Nanoscale Res Lett. 2018;13(1):360doi: 10.1186/s11671-018-2772-2.
Brzezicka, K. A., Serna, S., & Reichardt, N. C. (2018). Fluorescent Neoglycoprotein Gold Nanoclusters: Synthesis and Applications in Plant Lectin Sensing and Cell Imaging. Nanoscale research letters, 13(1), 360. https://doi.org/10.1186/s11671-018-2772-2
Brzezicka, Katarzyna Alicja, et al. "Fluorescent Neoglycoprotein Gold Nanoclusters: Synthesis and Applications in Plant Lectin Sensing and Cell Imaging." Nanoscale research letters vol. 13,1 (2018): 360. doi: https://doi.org/10.1186/s11671-018-2772-2
Brzezicka KA, Serna S, Reichardt NC. Fluorescent Neoglycoprotein Gold Nanoclusters: Synthesis and Applications in Plant Lectin Sensing and Cell Imaging. Nanoscale Res Lett. 2018 Nov 12;13(1):360. doi: 10.1186/s11671-018-2772-2. PMID: 30421263; PMCID: PMC6232188.
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Nanoscale Res Lett. 2018 Nov 12;13(1):360. doi: 10.1186/s11671-018-2772-2.

Fluorescent Neoglycoprotein Gold Nanoclusters: Synthesis and Applications in Plant Lectin Sensing and Cell Imaging.

Nanoscale research letters

Katarzyna Alicja Brzezicka, Sonia Serna, Niels Christian Reichardt

Affiliations

  1. Glycotechnology Laboratory, CIC biomaGUNE, Paseo Miramon 182, 20014, San Sebastian, Spain. [email protected].
  2. Departments of Molecular Medicine and Microbiology and Immunology, The Scripps Research Institute, La Jolla, CA, 92037, USA. [email protected].
  3. Glycotechnology Laboratory, CIC biomaGUNE, Paseo Miramon 182, 20014, San Sebastian, Spain.
  4. Glycotechnology Laboratory, CIC biomaGUNE, Paseo Miramon 182, 20014, San Sebastian, Spain. [email protected].
  5. CIBER-BBN, Paseo Miramon 182, 20014, San Sebastian, Spain. [email protected].

PMID: 30421263 PMCID: PMC6232188 DOI: 10.1186/s11671-018-2772-2

Abstract

Carbohydrate-protein interactions mediate fundamental biological processes, such as fertilization, cell signaling, or host-pathogen communication. However, because of the enormous complexity of glycan recognition events, new tools enabling their analysis or applications emerge in recent years. Here, we describe the first preparation of neoglycoprotein functionalized fluorescent gold nanoclusters, containing a biantennary N-glycan G0 as targeting molecule, ovalbumin as carrier/model antigen, and a fluorescent gold core as imaging probe (G0-OVA-AuNCs). Subsequently, we demonstrate the utility of generated G0-OVA-AuNCs for specific sensing of plant lectins and in vitro imaging of dendritic cells.

Keywords: Carbohydrate-protein interactions; Dendritic cells; Gold nanoclusters; Lectin sensing; Neoglycoproteins; Targeting

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