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Mustafa AG, Alfaqih MA, Al-Shboul O, et al. Scavenging of lipid peroxyl radicals protects plasma lipids and proteins from peroxynitrite. Biomed Rep. 2018;9(5):421-426doi: 10.3892/br.2018.1144.
Mustafa, A. G., Alfaqih, M. A., Al-Shboul, O., & Al-Dwairi, A. (2018). Scavenging of lipid peroxyl radicals protects plasma lipids and proteins from peroxynitrite. Biomedical reports, 9(5), 421-426. https://doi.org/10.3892/br.2018.1144
Mustafa, Ayman G, et al. "Scavenging of lipid peroxyl radicals protects plasma lipids and proteins from peroxynitrite." Biomedical reports vol. 9,5 (2018): 421-426. doi: https://doi.org/10.3892/br.2018.1144
Mustafa AG, Alfaqih MA, Al-Shboul O, Al-Dwairi A. Scavenging of lipid peroxyl radicals protects plasma lipids and proteins from peroxynitrite. Biomed Rep. 2018 Nov;9(5):421-426. doi: 10.3892/br.2018.1144. Epub 2018 Sep 07. PMID: 30345039; PMCID: PMC6176101.
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Biomed Rep. 2018 Nov;9(5):421-426. doi: 10.3892/br.2018.1144. Epub 2018 Sep 07.

Scavenging of lipid peroxyl radicals protects plasma lipids and proteins from peroxynitrite.

Biomedical reports

Ayman G Mustafa, Mahmoud A Alfaqih, Othman Al-Shboul, Ahmed Al-Dwairi

Affiliations

  1. Department of Anatomy, School of Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan.
  2. Department of Physiology and Biochemistry, School of Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan.

PMID: 30345039 PMCID: PMC6176101 DOI: 10.3892/br.2018.1144

Abstract

Peroxynitrite can be produced in the vasculature from a superoxide anion reaction with nitric oxide. A surplus of peroxynitrite in the intravascular compartment is a common feature of several chronic diseases. The development of pharmacological modalities that interfere with the formation of peroxynitrite or inhibit its oxidative damage may be of utility for the prevention and/or treatment of several pathologies. Our previous investigations showed that catalytically inactivating peroxynitrite-derived free radicals with tempol or scavenging reactive aldehyde species with phenelzine protects the blood plasma and platelets from the oxidative damage of peroxynitrite. However, the degree of inhibition of the cytotoxic effects of peroxynitrite using tempol or phenelzine was modest. In the present study, the aim was to examine if scavenging lipid peroxyl radicals with U-83836E can achieve superior protection from peroxynitrite. This was assessed by treating blood plasma or platelets with 100 µM peroxynitrite alone or in combination with U-83836E, and then measuring the levels of thiobarbituric acid reactive substances (TBARS) and protein carbonyl formation as indices of lipid peroxidation and protein oxidation, respectively. It was observed that scavenging lipid peroxyl radicals with 75-100 µM U-83836E increasingly reversed protein carbonylation induced by peroxynitrite in blood plasma and platelets, in addition to TBARS formation in blood plasma. These findings are further discussed in the context of the mechanisms by which U-83836E may protect against the cell-damaging effects of peroxynitrite.

Keywords: lipid peroxyl radicals; oxidative stress; peroxynitrite; reactive oxygen species; thiobarbituric acid reactive substances

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