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Sklodowska K, Debski PR, Michalski JA, et al. Simultaneous Measurement of Viscosity and Optical Density of Bacterial Growth and Death in a Microdroplet. Micromachines (Basel). 2018;9(5)doi: 10.3390/mi9050251.
Sklodowska, K., Debski, P. R., Michalski, J. A., Korczyk, P. M., Dolata, M., Zajac, M., & Jakiela, S. (2018). Simultaneous Measurement of Viscosity and Optical Density of Bacterial Growth and Death in a Microdroplet. Micromachines, 9(5), . https://doi.org/10.3390/mi9050251
Sklodowska, Karolina, et al. "Simultaneous Measurement of Viscosity and Optical Density of Bacterial Growth and Death in a Microdroplet." Micromachines vol. 9,5 (2018). doi: https://doi.org/10.3390/mi9050251
Sklodowska K, Debski PR, Michalski JA, Korczyk PM, Dolata M, Zajac M, Jakiela S. Simultaneous Measurement of Viscosity and Optical Density of Bacterial Growth and Death in a Microdroplet. Micromachines (Basel). 2018 May 21;9(5). doi: 10.3390/mi9050251. PMID: 30424184; PMCID: PMC6187717.
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Micromachines (Basel). 2018 May 21;9(5). doi: 10.3390/mi9050251.

Simultaneous Measurement of Viscosity and Optical Density of Bacterial Growth and Death in a Microdroplet.

Micromachines

Karolina Sklodowska, Pawel R Debski, Jacek A Michalski, Piotr M Korczyk, Miroslaw Dolata, Miroslaw Zajac, Slawomir Jakiela

Affiliations

  1. Department of Biophysics, Warsaw University of Life Sciences, 159 Nowoursynowska Street, 02776 Warsaw, Poland. [email protected].
  2. Department of Plant Genetics, Breeding and Biotechnology, Warsaw University of Life Sciences, 159 Nowoursynowska Street, 02776 Warsaw, Poland. [email protected].
  3. Department of Biophysics, Warsaw University of Life Sciences, 159 Nowoursynowska Street, 02776 Warsaw, Poland. [email protected].
  4. Faculty of Civil Engineering, Mechanics and Petrochemistry, Warsaw University of Technology, 17 Lukasiewicza Street, 09400 Plock, Poland. [email protected].
  5. Institute of Fundamental Technological Research, Polish Academy of Sciences, Pawinskiego 5B, 02106 Warsaw, Poland. [email protected].
  6. Department of Econophysics and Physics Application, Warsaw University of Life Sciences, 159 Nowoursynowska Street, 02776 Warsaw, Poland. [email protected].
  7. Department of Biophysics, Warsaw University of Life Sciences, 159 Nowoursynowska Street, 02776 Warsaw, Poland. [email protected].
  8. Department of Biophysics, Warsaw University of Life Sciences, 159 Nowoursynowska Street, 02776 Warsaw, Poland. [email protected].

PMID: 30424184 PMCID: PMC6187717 DOI: 10.3390/mi9050251

Abstract

Herein, we describe a novel method for the assessment of droplet viscosity moving inside microfluidic channels. The method allows for the monitoring of the rate of the continuous growth of bacterial culture. It is based on the analysis of the hydrodynamic resistance of a droplet that is present in a microfluidic channel, which affects its motion. As a result, we were able to observe and quantify the change in the viscosity of the dispersed phase that is caused by the increasing population of interacting bacteria inside a size-limited system. The technique allows for finding the correlation between the viscosity of the medium with a bacterial culture and its optical density. These features, together with the high precision of the measurement, make our viscometer a promising tool for various experiments in the field of analytical chemistry and microbiology, where the rigorous control of the conditions of the reaction and the monitoring of the size of bacterial culture are vital.

Keywords: Escherichia coli; cell growth; droplet microfluidics; rheology; viscosity

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