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Debski PR, Sklodowska K, Michalski JA, et al. Continuous Recirculation of Microdroplets in a Closed Loop Tailored for Screening of Bacteria Cultures. Micromachines (Basel). 2018;9(9)doi: 10.3390/mi9090469.
Debski, P. R., Sklodowska, K., Michalski, J. A., Korczyk, P. M., Dolata, M., & Jakiela, S. (2018). Continuous Recirculation of Microdroplets in a Closed Loop Tailored for Screening of Bacteria Cultures. Micromachines, 9(9), . https://doi.org/10.3390/mi9090469
Debski, Pawel R, et al. "Continuous Recirculation of Microdroplets in a Closed Loop Tailored for Screening of Bacteria Cultures." Micromachines vol. 9,9 (2018). doi: https://doi.org/10.3390/mi9090469
Debski PR, Sklodowska K, Michalski JA, Korczyk PM, Dolata M, Jakiela S. Continuous Recirculation of Microdroplets in a Closed Loop Tailored for Screening of Bacteria Cultures. Micromachines (Basel). 2018 Sep 17;9(9). doi: 10.3390/mi9090469. PMID: 30424402; PMCID: PMC6187375.
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Micromachines (Basel). 2018 Sep 17;9(9). doi: 10.3390/mi9090469.

Continuous Recirculation of Microdroplets in a Closed Loop Tailored for Screening of Bacteria Cultures.

Micromachines

Pawel R Debski, Karolina Sklodowska, Jacek A Michalski, Piotr M Korczyk, Miroslaw Dolata, Slawomir Jakiela

Affiliations

  1. Department of Biophysics, Warsaw University of Life Sciences, 159 Nowoursynowska Street, 02776 Warsaw, Poland. [email protected].
  2. Department of Biophysics, Warsaw University of Life Sciences, 159 Nowoursynowska Street, 02776 Warsaw, Poland. [email protected].
  3. Department of Plant Genetics, Breeding and Biotechnology, Warsaw University of Life Sciences, 159 Nowoursynowska Street, 02776 Warsaw, Poland. [email protected].
  4. Faculty of Civil Engineering, Mechanics and Petrochemistry, Warsaw University of Technology, 17 Lukasiewicza Street, 09400 Plock, Poland. [email protected].
  5. Institute of Fundamental Technological Research, Polish Academy of Sciences, Pawinskiego 5B, 02106 Warsaw, Poland. [email protected].
  6. Department of Econophysics and Physics Application, Warsaw University of Life Sciences, 159 Nowoursynowska Street, 02776 Warsaw, Poland. [email protected].
  7. Department of Biophysics, Warsaw University of Life Sciences, 159 Nowoursynowska Street, 02776 Warsaw, Poland. [email protected].

PMID: 30424402 PMCID: PMC6187375 DOI: 10.3390/mi9090469

Abstract

Emerging microfluidic technology has introduced new precision controls over reaction conditions. Owing to the small amount of reagents, microfluidics significantly lowers the cost of carrying a single reaction. Moreover, in two-phase systems, each part of a dispersed fluid can be treated as an independent chemical reactor with a volume from femtoliters to microliters, increasing the throughput. In this work, we propose a microfluidic device that provides continuous recirculation of droplets in a closed loop, maintaining low consumption of oil phase, no cross-contamination, stabilized temperature, a constant condition of gas exchange, dynamic feedback control on droplet volume, and a real-time optical characterization of bacterial growth in a droplet. The channels (tubing) and junction cubes are made of Teflon fluorinated ethylene propylene (FEP) to ensure non-wetting conditions and to prevent the formation of biofilm, which is particularly crucial for biological experiments. We show the design and operation of a novel microfluidic loop with the circular motion of microdroplet reactors monitored with optical sensors and precision temperature controls. We have employed the proposed system for long term monitoring of bacterial growth during the antibiotic chloramphenicol treatment. The proposed system can find applications in a broad field of biomedical diagnostics and therapy.

Keywords: Escherichia coli; antibiotic treatment; bacteria cultures; microfluidic loop; screening

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