Display options
Cite
Zhang Z, Li Z, Chen W. Down-regulated G protein-coupled receptor kinase 6 leads to apoptosis in multiple myeloma MM1R cells. Exp Ther Med. 2018;16(5):4253-4259doi: 10.3892/etm.2018.6722.
Zhang, Z., Li, Z., & Chen, W. (2018). Down-regulated G protein-coupled receptor kinase 6 leads to apoptosis in multiple myeloma MM1R cells. Experimental and therapeutic medicine, 16(5), 4253-4259. https://doi.org/10.3892/etm.2018.6722
Zhang, Zhiyao, et al. "Down-regulated G protein-coupled receptor kinase 6 leads to apoptosis in multiple myeloma MM1R cells." Experimental and therapeutic medicine vol. 16,5 (2018): 4253-4259. doi: https://doi.org/10.3892/etm.2018.6722
Zhang Z, Li Z, Chen W. Down-regulated G protein-coupled receptor kinase 6 leads to apoptosis in multiple myeloma MM1R cells. Exp Ther Med. 2018 Nov;16(5):4253-4259. doi: 10.3892/etm.2018.6722. Epub 2018 Sep 11. PMID: 30402162; PMCID: PMC6201053.
Copy Download .nbib Format: NLM
Share it on

Exp Ther Med. 2018 Nov;16(5):4253-4259. doi: 10.3892/etm.2018.6722. Epub 2018 Sep 11.

Down-regulated G protein-coupled receptor kinase 6 leads to apoptosis in multiple myeloma MM1R cells.

Experimental and therapeutic medicine

Zhiyao Zhang, Zhenyu Li, Wenming Chen

Affiliations

  1. Department of Hematology, Beijing Chao Yang Hospital, Capital Medical University, Beijing 100020, P.R. China.
  2. Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221002, P.R. China.

PMID: 30402162 PMCID: PMC6201053 DOI: 10.3892/etm.2018.6722

Abstract

G protein-coupled receptor kinase 6 (GRK6) is highly expressed in multiple myeloma (MM) cell lines, but absent or only weakly expressed in most primary human somatic cells. In the present study, GRK6 expression was assessed in MM patients and healthy individuals by quantitative polymerase chain reaction. Flow cytometry were performed to measure the apoptosis of lentivial-transfected MM1R cells. Western blot analysis was performed to assess the apoptosis and signal transducer and activator of transcription 3 pathway-related factors. The results demonstrated that GRK6 was differentially expressed in individuals who suffered from MM and healthy individuals. Previous studies have shown that downregulating GRK6 has anti-cancer effects in the MM cell line, MM1R. The present study demonstrated that RNA interference-mediated GRK6 knockdown promoted apoptosis in the MM1R cell line. Therefore, we hypothesized that GRK6 plays a significant role in determining the course of MM.

Keywords: GRK6; MM1R; STAT3; apoptosis; multiple myeloma

References

  1. Cancer Lett. 2007 Jun 28;251(2):199-210 - PubMed
  2. Asian Pac J Trop Med. 2013 Mar;6(3):220-3 - PubMed
  3. J Leukoc Biol. 2004 Apr;75(4):698-704 - PubMed
  4. J Biol Chem. 1992 Apr 5;267(10):6886-92 - PubMed
  5. Methods. 2001 Dec;25(4):402-8 - PubMed
  6. Mol Cancer Res. 2007 Sep;5(9):943-55 - PubMed
  7. Nat Commun. 2013;4:1532 - PubMed
  8. Blood. 2004 Apr 15;103(8):3175-84 - PubMed
  9. Blood. 2004 Jan 1;103(1):242-51 - PubMed
  10. Genome Biol. 2006;7(9):236 - PubMed
  11. Fag Tidsskr Sykepleien. 1990 May 29;78(4):26-8 - PubMed
  12. Br J Cancer. 2006 Jan 30;94(2):318-22 - PubMed
  13. Annu Rev Physiol. 2007;69:483-510 - PubMed
  14. Blood. 1996 Dec 15;88(12):4547-57 - PubMed
  15. Acta Physiol Hung. 1987;70(4):351-6 - PubMed
  16. Cell. 1999 Aug 6;98(3):295-303 - PubMed
  17. J Cancer Res Clin Oncol. 2016 Dec;142(12):2541-2549 - PubMed
  18. Blood. 2008 Dec 15;112(13):5095-102 - PubMed
  19. Cell. 2012 Aug 31;150(5):987-1001 - PubMed
  20. J Biol Chem. 1997 Jul 11;272(28):17734-43 - PubMed
  21. Mayo Clin Proc. 2016 Jan;91(1):101-19 - PubMed
  22. Proc Natl Acad Sci U S A. 2002 May 28;99(11):7478-83 - PubMed
  23. Kidney Int. 1987 May;31(5):1153-9 - PubMed
  24. Nat Rev Cancer. 2002 Mar;2(3):175-87 - PubMed
  25. Nat Genet. 2005 Jun;37(6):590-2 - PubMed
  26. Blood. 2008 Mar 1;111(5):2516-20 - PubMed
  27. Stem Cells Dev. 2015 Mar 15;24(6):737-46 - PubMed

Publication Types