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Kang WB, Chen YJ, Lu DY, et al. Folic acid contributes to peripheral nerve injury repair by promoting Schwann cell proliferation, migration, and secretion of nerve growth factor. Neural Regen Res. 2019;14(1):132-139doi: 10.4103/1673-5374.243718.
Kang, W. B., Chen, Y. J., Lu, D. Y., & Yan, J. Z. (2019). Folic acid contributes to peripheral nerve injury repair by promoting Schwann cell proliferation, migration, and secretion of nerve growth factor. Neural regeneration research, 14(1), 132-139. https://doi.org/10.4103/1673-5374.243718
Kang, Wei-Bo, et al. "Folic acid contributes to peripheral nerve injury repair by promoting Schwann cell proliferation, migration, and secretion of nerve growth factor." Neural regeneration research vol. 14,1 (2019): 132-139. doi: https://doi.org/10.4103/1673-5374.243718
Kang WB, Chen YJ, Lu DY, Yan JZ. Folic acid contributes to peripheral nerve injury repair by promoting Schwann cell proliferation, migration, and secretion of nerve growth factor. Neural Regen Res. 2019 Jan;14(1):132-139. doi: 10.4103/1673-5374.243718. PMID: 30531087; PMCID: PMC6263007.
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Neural Regen Res. 2019 Jan;14(1):132-139. doi: 10.4103/1673-5374.243718.

Folic acid contributes to peripheral nerve injury repair by promoting Schwann cell proliferation, migration, and secretion of nerve growth factor.

Neural regeneration research

Wei-Bo Kang, Yong-Jie Chen, Du-Yi Lu, Jia-Zhi Yan

Affiliations

  1. Department of Orthopedic Surgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

PMID: 30531087 PMCID: PMC6263007 DOI: 10.4103/1673-5374.243718

Abstract

After peripheral nerve injury, intraperitoneal injection of folic acid improves axon quantity, increases axon density and improves electromyography results. However, the mechanisms for this remain unclear. This study explored whether folic acid promotes peripheral nerve injury repair by affecting Schwann cell function. Primary Schwann cells were obtained from rats by in vitro separation and culture. Cell proliferation, assayed using the Cell Counting Kit-8 assay, was higher in cells cultured for 72 hours with 100 mg/L folic acid compared with the control group. Cell proliferation was also higher in the 50, 100, 150, and 200 mg/L folic acid groups compared with the control group after culture for 96 hours. Proliferation was markedly higher in the 100 mg/L folic acid group compared with the 50 mg/L folic acid group and the 40 ng/L nerve growth factor group. In Transwell assays, the number of migrated Schwann cells dramatically increased after culture with 100 and 150 mg/L folic acid compared with the control group. In nerve growth factor enzyme-linked immunosorbent assays, treatment of Schwann cell cultures with 50, 100, and 150 mg/L folic acid increased levels of nerve growth factor in the culture medium compared with the control group at 3 days. The nerve growth factor concentration of Schwann cell cultures treated with 100 mg/L folic acid group was remarkably higher than that in the 50 and 150 mg/L folic acid groups at 3 days. Nerve growth factor concentration in the 10, 50, and 100 mg/L folic acid groups was higher than that in the control group at 7 days. The nerve growth factor concentration in the 50 mg/L folic acid group was remarkably higher than that in the 10 and 100 mg/L folic acid groups at 7 days. In vivo, 80 μg/kg folic acid was intraperitoneally administrated for 7 consecutive days after sciatic nerve injury. Immunohistochemical staining showed that the number of Schwann cells in the folic acid group was greater than that in the control group. We suggest that folic acid may play a role in improving the repair of peripheral nerve injury by promoting the proliferation and migration of Schwann cells and the secretion of nerve growth factors.

Keywords: Schwann cell; biomaterial; cell functions; folic acid; nerve regeneration; neural regeneration; neurotrophic factor; peripheral nerve injury; peripheral nerve repair; tissue engineering

Conflict of interest statement

None

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