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Gómez GI, Falcon RV, Maturana CJ, et al. Heavy Alcohol Exposure Activates Astroglial Hemichannels and Pannexons in the Hippocampus of Adolescent Rats: Effects on Neuroinflammation and Astrocyte Arborization. Front Cell Neurosci. 2018;12:472doi: 10.3389/fncel.2018.00472.
Gómez, G. I., Falcon, R. V., Maturana, C. J., Labra, V. C., Salgado, N., Rojas, C. A., Oyarzun, J. E., Cerpa, W., Quintanilla, R. A., & Orellana, J. A. (2018). Heavy Alcohol Exposure Activates Astroglial Hemichannels and Pannexons in the Hippocampus of Adolescent Rats: Effects on Neuroinflammation and Astrocyte Arborization. Frontiers in cellular neuroscience, 12472. https://doi.org/10.3389/fncel.2018.00472
Gómez, Gonzalo I, et al. "Heavy Alcohol Exposure Activates Astroglial Hemichannels and Pannexons in the Hippocampus of Adolescent Rats: Effects on Neuroinflammation and Astrocyte Arborization." Frontiers in cellular neuroscience vol. 12 (2018): 472. doi: https://doi.org/10.3389/fncel.2018.00472
Gómez GI, Falcon RV, Maturana CJ, Labra VC, Salgado N, Rojas CA, Oyarzun JE, Cerpa W, Quintanilla RA, Orellana JA. Heavy Alcohol Exposure Activates Astroglial Hemichannels and Pannexons in the Hippocampus of Adolescent Rats: Effects on Neuroinflammation and Astrocyte Arborization. Front Cell Neurosci. 2018 Dec 04;12:472. doi: 10.3389/fncel.2018.00472. eCollection 2018. PMID: 30564103; PMCID: PMC6288256.
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Front Cell Neurosci. 2018 Dec 04;12:472. doi: 10.3389/fncel.2018.00472. eCollection 2018.

Heavy Alcohol Exposure Activates Astroglial Hemichannels and Pannexons in the Hippocampus of Adolescent Rats: Effects on Neuroinflammation and Astrocyte Arborization.

Frontiers in cellular neuroscience

Gonzalo I Gómez, Romina V Falcon, Carola J Maturana, Valeria C Labra, Nicole Salgado, Consuelo A Rojas, Juan E Oyarzun, Waldo Cerpa, Rodrigo A Quintanilla, Juan A Orellana

Affiliations

  1. Departamento de Neurología, Escuela de Medicina and Centro Interdisciplinario de Neurociencias, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
  2. Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Santiago, Chile.
  3. Unidad de Microscopía Avanzada Medicina, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
  4. Laboratorio de Función y Patología Neuronal, Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
  5. Centro de Investigación y Estudio del Consumo de Alcohol en Adolescentes (CIAA), Santiago, Chile.
  6. Laboratory of Neurodegenerative Diseases, Universidad Autónoma de Chile, Santiago, Chile.

PMID: 30564103 PMCID: PMC6288256 DOI: 10.3389/fncel.2018.00472

Abstract

A mounting body of evidence indicates that adolescents are specially more susceptible to alcohol influence than adults. However, the mechanisms underlying this phenomenon remain poorly understood. Astrocyte-mediated gliotransmission is crucial for hippocampal plasticity and recently, the opening of hemichannels and pannexons has been found to participate in both processes. Here, we evaluated whether adolescent rats exposed to ethanol exhibit changes in the activity of astrocyte hemichannels and pannexons in the hippocampus, as well as alterations in astrocyte arborization and cytokine levels. Adolescent rats were subjected to ethanol (3.0 g/kg) for two successive days at 48-h periods over 14 days. The opening of hemichannels and pannexons was examined in hippocampal slices by dye uptake, whereas hippocampal cytokine levels and astroglial arborization were determined by ELISA and Sholl analysis, respectively. We found that adolescent ethanol exposure increased the opening of connexin 43 (Cx43) hemichannels and pannexin-1 (Panx1) channels in astrocytes. Blockade of p38 mitogen-activated protein kinase (MAPK), inducible nitric oxide synthase (iNOS) and cyclooxygenases (COXs), as well as chelation of intracellular Ca

Keywords: alcoholism; astrocyte; connexins; cytokines; glia; hippocampus; pannexins

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