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Mercati F, Maranesi M, Dall'Aglio C, et al. Apelin System in Mammary Gland of Sheep Reared in Semi-Natural Pastures of the Central Apennines. Animals (Basel). 2018;8(12)doi: 10.3390/ani8120223.
Mercati, F., Maranesi, M., Dall'Aglio, C., Petrucci, L., Pasquariello, R., Tardella, F. M., De Felice, E., & Scocco, P. (2018). Apelin System in Mammary Gland of Sheep Reared in Semi-Natural Pastures of the Central Apennines. Animals : an open access journal from MDPI, 8(12), . https://doi.org/10.3390/ani8120223
Mercati, Francesca, et al. "Apelin System in Mammary Gland of Sheep Reared in Semi-Natural Pastures of the Central Apennines." Animals : an open access journal from MDPI vol. 8,12 (2018). doi: https://doi.org/10.3390/ani8120223
Mercati F, Maranesi M, Dall'Aglio C, Petrucci L, Pasquariello R, Tardella FM, De Felice E, Scocco P. Apelin System in Mammary Gland of Sheep Reared in Semi-Natural Pastures of the Central Apennines. Animals (Basel). 2018 Nov 28;8(12). doi: 10.3390/ani8120223. PMID: 30486490; PMCID: PMC6315652.
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Animals (Basel). 2018 Nov 28;8(12). doi: 10.3390/ani8120223.

Apelin System in Mammary Gland of Sheep Reared in Semi-Natural Pastures of the Central Apennines.

Animals : an open access journal from MDPI

Francesca Mercati, Margherita Maranesi, Cecilia Dall'Aglio, Linda Petrucci, Rolando Pasquariello, Federico Maria Tardella, Elena De Felice, Paola Scocco

Affiliations

  1. Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy. [email protected].
  2. Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy. [email protected].
  3. Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy. [email protected].
  4. Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy. [email protected].
  5. Animal Reproduction and Biotechnology Laboratory, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, 1683 Campus delivery, Fort Collins, CO 80523, USA. [email protected].
  6. School of Biosciences and Veterinary Medicine, University of Camerino, Via Pontoni 5, 62032 Camerino, Italy. [email protected].
  7. School of Biosciences and Veterinary Medicine, University of Camerino, Via Pontoni 5, 62032 Camerino, Italy. [email protected].
  8. School of Biosciences and Veterinary Medicine, University of Camerino, Via Pontoni 5, 62032 Camerino, Italy. [email protected].

PMID: 30486490 PMCID: PMC6315652 DOI: 10.3390/ani8120223

Abstract

Sheep are the most bred species in the Central Italy Apennine using the natural pastures as a trophic resource and grazing activity is fundamental to maintain the grassland biodiversity: this goal can be reached only ensuring an economical sustainability to the farmers. This study aimed to investigate the apelin/apelin receptor system in ovine mammary gland and to evaluate the differences induced by food supplementation, in order to shed light on this system function. A flock of 15 Comisana x Appenninica adult dry ewes were free to graze from June until pasture maximum flowering (MxF). From this period to pasture maximum dryness (MxD), in addition to grazing, the experimental group (Exp) was supplemented with 600 g/day/head of cereals. Apelin and apelin receptor were assessed by Real-Time PCR and immunohistochemistry on the mammary glands of subjects pertaining to MxF, MxD and Exp groups. They were detected in alveolar and ductal epithelial cells. The pasture maximum flowering group showed significant differences in apelin expression compared with experimental and MxD groups. Apelin receptor expression significantly differed among the three groups. The reduced apelin receptor expression and immunoreactivity levels during parenchyma involution enables us to hypothesize that apelin receptor plays a modulating role in the system control.

Keywords: Apennine grasslands; Ovis aries; apelin; apelin receptor; drought stress; mammary gland

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