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Gholamnezhad Z, Fatehi Hassanabad Z. Effects of lipopolysaccharide-induced septic shock on rat isolated kidney, possible role of nitric oxide and protein kinase C pathways. Iran J Basic Med Sci. 2018;21(10):1073-1078doi: 10.22038/IJBMS.2018.27798.6773.
Gholamnezhad, Z., & Fatehi Hassanabad, Z. (2018). Effects of lipopolysaccharide-induced septic shock on rat isolated kidney, possible role of nitric oxide and protein kinase C pathways. Iranian journal of basic medical sciences, 21(10), 1073-1078. https://doi.org/10.22038/IJBMS.2018.27798.6773
Gholamnezhad, Zahra, and Fatehi Hassanabad, Zahra. "Effects of lipopolysaccharide-induced septic shock on rat isolated kidney, possible role of nitric oxide and protein kinase C pathways." Iranian journal of basic medical sciences vol. 21,10 (2018): 1073-1078. doi: https://doi.org/10.22038/IJBMS.2018.27798.6773
Gholamnezhad Z, Fatehi Hassanabad Z. Effects of lipopolysaccharide-induced septic shock on rat isolated kidney, possible role of nitric oxide and protein kinase C pathways. Iran J Basic Med Sci. 2018 Oct;21(10):1073-1078. doi: 10.22038/IJBMS.2018.27798.6773. PMID: 30524682; PMCID: PMC6281070.
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Iran J Basic Med Sci. 2018 Oct;21(10):1073-1078. doi: 10.22038/IJBMS.2018.27798.6773.

Effects of lipopolysaccharide-induced septic shock on rat isolated kidney, possible role of nitric oxide and protein kinase C pathways.

Iranian journal of basic medical sciences

Zahra Gholamnezhad, Zahra Fatehi Hassanabad

Affiliations

  1. Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
  2. Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
  3. Emam zaman Hospital, Mashhad, Iran.

PMID: 30524682 PMCID: PMC6281070 DOI: 10.22038/IJBMS.2018.27798.6773

Abstract

OBJECTIVES: Pathophysiology of sepsis-associated renal failure (one of the most common cause of death in intensive care units) had not been fully determined. The effect of nitric oxide and protein kinase C (PKC) pathways in isolated kidney of Lipopolysaccharide-treated (LPS) rats were investigated in this study.

MATERIALS AND METHODS: Vascular responsiveness to phenylephrine and acetylcholine in the presence and absence of a potent PKC inhibitor (chelerythrine) and nonspecific NO inhibitor (L-NAME) as well as responses to acetylcholine and sodium nitroprusside (SNP) were examined.

RESULTS: LPS (10 mg/kg, IP) treatment resulted in a lower systemic pressure and reduction of responses to vasoconstrictor and vasodilator agents (

CONCLUSION: Present study highlighted that five hours of intraperitoneal endotoxin injection is adequate to reduce renal basal perfusion pressure. These results also suggest that PKC inhibition may have a beneficial role in vascular hyporesponsiveness induced by LPS. Although our study partly elaborated on the effects of LPS on isolated renal vascular responses to vasoactive agents, further studies are required to explain how LPS exerts its renal vascular effects.

Keywords: Kidney; Lipopolysaccharide; Nitric oxide; Protein kinase C; Rat; Vasoconstrictor; Vasodilator

Conflict of interest statement

The authors declare no conflict of interest in this study.

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