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Krishnaswamy A, Barnes N, Lotlikar NP, et al. An Improved Method for Protein Extraction from Minuscule Quantities of Fungal Biomass. Indian J Microbiol. 2018;59(1):100-104doi: 10.1007/s12088-018-0752-y.
Krishnaswamy, A., Barnes, N., Lotlikar, N. P., & Damare, S. R. (2019). An Improved Method for Protein Extraction from Minuscule Quantities of Fungal Biomass. Indian journal of microbiology, 59(1), 100-104. https://doi.org/10.1007/s12088-018-0752-y
Krishnaswamy, Akhila, et al. "An Improved Method for Protein Extraction from Minuscule Quantities of Fungal Biomass." Indian journal of microbiology vol. 59,1 (2019): 100-104. doi: https://doi.org/10.1007/s12088-018-0752-y
Krishnaswamy A, Barnes N, Lotlikar NP, Damare SR. An Improved Method for Protein Extraction from Minuscule Quantities of Fungal Biomass. Indian J Microbiol. 2019 Mar;59(1):100-104. doi: 10.1007/s12088-018-0752-y. Epub 2018 Jun 26. PMID: 30728637; PMCID: PMC6328415.
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Indian J Microbiol. 2019 Mar;59(1):100-104. doi: 10.1007/s12088-018-0752-y. Epub 2018 Jun 26.

An Improved Method for Protein Extraction from Minuscule Quantities of Fungal Biomass.

Indian journal of microbiology

Akhila Krishnaswamy, Natasha Barnes, Nikita P Lotlikar, Samir R Damare

Affiliations

  1. 1Biological Oceanography Division, CSIR-National Institute of Oceanography, Dona Paula, Goa 403004 India.
  2. 2Present Address: Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong.

PMID: 30728637 PMCID: PMC6328415 DOI: 10.1007/s12088-018-0752-y

Abstract

Filamentous fungi are ubiquitous eukaryotes having chitin as a major constituent of the cell wall. Chitin is tough to lyse due to which the intracellular fungal proteins are not readily accessible. The problem is further enhanced when the biomass to be analyzed for protein studies is too little due to the extreme experimental parameters under consideration such as increased or lowered pH, temperature, hydrostatic pressure, nutrients, etc. The method described here is capable of obtaining proteins from minuscule quantities of biomass (~5 mg lyophilized biomass). In this study, different lysing conditions and varied composition of extraction buffers were tried to obtain maximum protein of high quality. Lysis with zirconium beads in a combination buffer system (Tris-MgCl

Keywords: Biomass; Fungi; Homogenizer; Protein; Zirconium beads

Conflict of interest statement

Compliance with ethical standardsThe authors declare that they have no conflict of interest.

References

  1. Bioprocess Technol. 1991;12:57-83 - PubMed
  2. Mycologia. 2008 Nov-Dec;100(6):867-75 - PubMed
  3. J Microbiol Methods. 2012 Mar;88(3):413-8 - PubMed
  4. Indian J Microbiol. 2010 Oct;50(Suppl 1):127-31 - PubMed
  5. Electrophoresis. 1997 Mar-Apr;18(3-4):307-16 - PubMed

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