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Silva ADA, Pinho DB, Costa H, et al. First Report of Leaf Spot Caused by Myrothecium roridum on Coffea canephora in Brazil. Plant Dis. 2014;98(11):1587doi: 10.1094/PDIS-06-14-0643-PDN.
Silva, A. D. A., Pinho, D. B., Costa, H., Lopes, U. P., & Pereira, O. L. (2014). First Report of Leaf Spot Caused by Myrothecium roridum on Coffea canephora in Brazil. Plant disease, 98(11), 1587. https://doi.org/10.1094/PDIS-06-14-0643-PDN
Silva, A D A, et al. "First Report of Leaf Spot Caused by Myrothecium roridum on Coffea canephora in Brazil." Plant disease vol. 98,11 (2014): 1587. doi: https://doi.org/10.1094/PDIS-06-14-0643-PDN
Silva ADA, Pinho DB, Costa H, Lopes UP, Pereira OL. First Report of Leaf Spot Caused by Myrothecium roridum on Coffea canephora in Brazil. Plant Dis. 2014 Nov;98(11):1587. doi: 10.1094/PDIS-06-14-0643-PDN. PMID: 30699835.
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Plant Dis. 2014 Nov;98(11):1587. doi: 10.1094/PDIS-06-14-0643-PDN.

First Report of Leaf Spot Caused by Myrothecium roridum on Coffea canephora in Brazil.

Plant disease

A D A Silva, D B Pinho, H Costa, U P Lopes, O L Pereira

Affiliations

  1. Departamento de Fitopatologia, Universidade Federal de Viçosa, Minas Gerais 36570-900, Brazil.
  2. Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural, Espírito Santo 29375-000, Brazil.

PMID: 30699835 DOI: 10.1094/PDIS-06-14-0643-PDN

Abstract

Coffea canephora (conilon coffee) represents approximately 30% of the coffee marketed worldwide. The state of Espírito Santo is the largest conilon coffee-producing state in Brazil. In 2013 and 2014, leaves with a leaf spot were observed on most of the conilon coffee seedlings in a commercial nursery in Laranja da Terra, Espírito Santo, Brazil. The infected leaves were deposited in the VIC Herbarium (VIC 42482) and a pure single-spore culture of the pathogen was deposited in the culture collection of the Universidade Federal de Viçosa (Accession No. COAD 1729). The initial symptoms were circular, brown to dark brown lesions with yellow margins occurring on both leaf surfaces. In high humidity, concentric rings formed and the lesions expanded rapidly to reach up to 30 mm in diameter, and later became dark brown with a grayish center. Black sporodochia with white, and marginal mycelial tuffs bearing black spore masses were observed in the older lesions. These symptoms were consistent with those of Myrothecium leaf spot reported on Coffea spp. (3). Microscopic observation revealed aseptate, hyaline, and cylindrical conidia, rounded at both ends, greenish to black in mass, and 5 to 6 μm long and 1 to 2 μm wide. The symptoms and morphological characteristics described above matched the description of Myrothecium roridum Tode (4). To confirm this identification, DNA was extracted using a Wizard Genomic DNA Purification Kit and the sequence of an internal transcribed spacer (ITS) region was obtained and deposited in GenBank (Accession No. KJ815095). The sequence of the ITS region exhibited 100% identity over 561 bp with another M. roridum sequence in GenBank (JF343832). To verify the pathogenicity of the fungus, healthy leaves of the C. canephora clones 12v and 14 (four seedlings each) were wounded superficially with a sterilized needle and inoculated by spraying them with a suspension of M. roridum conidia (10

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