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Monteiro-Hara ACBA, Jadão AS, Mendes BMJ, et al. Genetic Transformation of Passionflower and Evaluation of R. Plant Dis. 2011;95(8):1021-1025doi: 10.1094/PDIS-12-10-0873.
Monteiro-Hara, A. C. B. A., Jadão, A. S., Mendes, B. M. J., Rezende, J. A. M., Trevisan, F., Mello, A. P. O. A., Vieira, M. L. C., Meletti, L. M. M., & De S Piedade, S. M. (2011). Genetic Transformation of Passionflower and Evaluation of R. Plant disease, 95(8), 1021-1025. https://doi.org/10.1094/PDIS-12-10-0873
Monteiro-Hara, Alessandra C B A, et al. "Genetic Transformation of Passionflower and Evaluation of R." Plant disease vol. 95,8 (2011): 1021-1025. doi: https://doi.org/10.1094/PDIS-12-10-0873
Monteiro-Hara ACBA, Jadão AS, Mendes BMJ, Rezende JAM, Trevisan F, Mello APOA, Vieira MLC, Meletti LMM, De S Piedade SM. Genetic Transformation of Passionflower and Evaluation of R. Plant Dis. 2011 Aug;95(8):1021-1025. doi: 10.1094/PDIS-12-10-0873. PMID: 30732105.
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Plant Dis. 2011 Aug;95(8):1021-1025. doi: 10.1094/PDIS-12-10-0873.

Genetic Transformation of Passionflower and Evaluation of R.

Plant disease

Alessandra C B A Monteiro-Hara, Adriana S Jadão, Beatriz M J Mendes, Jorge A M Rezende, Flavio Trevisan, Ana Paula O A Mello, Maria Lucia C Vieira, L M M Meletti, Sônia M De S Piedade

Affiliations

  1. Laboratório de Biotecnologia Vegetal, Centro de Energia Nuclear na Agricultura, Universidade de São Paulo (CENA/USP), 13400-970 Piracicaba, SP, Brazil.
  2. Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo (ESALQ/USP), 13418-900 Piracicaba, SP, Brazil.
  3. Laboratório de Biotecnologia Vegetal, CENA/USP.
  4. Departamento de Fitopatologia e Nematologia, ESALQ/USP.
  5. Departamento de Genética, ESALQ/USP, 13418-900 Piracicaba, SP, Brazil.
  6. Centro de Fruticultura, Instituto Agronômico, 13020-902 Campinas, SP, Brazil.
  7. Departamento de Ciências Exatas, ESALQ/USP.

PMID: 30732105 DOI: 10.1094/PDIS-12-10-0873

Abstract

We report on the production and evaluation of passionflower transgenic lines for resistance to Cowpea aphid borne mosaic virus (CABMV). Genetic transformation was done using Agrobacterium tumefaciens and transgene integration was confirmed by Southern blot analyses, resulting in nine transgenic lines for 'IAC 275' and three for 'IAC 277'. Transgenic lines were clonally propagated and evaluated for resistance to CABMV. After the third inoculation, under higher inoculum pressure, only propagated plants of the transgenic line T16 remained asymptomatic, indicating a high resistance to infection with CABMV. This transgenic line was self-pollinated and the R

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