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Taniguchi Y, Yoshioka T, Sugaya H, et al. Growth factor levels in leukocyte-poor platelet-rich plasma and correlations with donor age, gender, and platelets in the Japanese population. J Exp Orthop. 2019;6(1):4doi: 10.1186/s40634-019-0175-7.
Taniguchi, Y., Yoshioka, T., Sugaya, H., Gosho, M., Aoto, K., Kanamori, A., & Yamazaki, M. (2019). Growth factor levels in leukocyte-poor platelet-rich plasma and correlations with donor age, gender, and platelets in the Japanese population. Journal of experimental orthopaedics, 6(1), 4. https://doi.org/10.1186/s40634-019-0175-7
Taniguchi, Yu, et al. "Growth factor levels in leukocyte-poor platelet-rich plasma and correlations with donor age, gender, and platelets in the Japanese population." Journal of experimental orthopaedics vol. 6,1 (2019): 4. doi: https://doi.org/10.1186/s40634-019-0175-7
Taniguchi Y, Yoshioka T, Sugaya H, Gosho M, Aoto K, Kanamori A, Yamazaki M. Growth factor levels in leukocyte-poor platelet-rich plasma and correlations with donor age, gender, and platelets in the Japanese population. J Exp Orthop. 2019 Feb 02;6(1):4. doi: 10.1186/s40634-019-0175-7. PMID: 30712144; PMCID: PMC6359998.
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J Exp Orthop. 2019 Feb 02;6(1):4. doi: 10.1186/s40634-019-0175-7.

Growth factor levels in leukocyte-poor platelet-rich plasma and correlations with donor age, gender, and platelets in the Japanese population.

Journal of experimental orthopaedics

Yu Taniguchi, Tomokazu Yoshioka, Hisashi Sugaya, Masahiko Gosho, Katsuya Aoto, Akihiro Kanamori, Masashi Yamazaki

Affiliations

  1. Department of Orthopaedic Surgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.
  2. Musculoskeletal System, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.
  3. Department of Orthopaedic Surgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan. [email protected].
  4. Musculoskeletal System, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan. [email protected].
  5. Department of Clinical Trial and Clinical Epidemiology, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.

PMID: 30712144 PMCID: PMC6359998 DOI: 10.1186/s40634-019-0175-7

Abstract

BACKGROUND: Clinical application of platelet-rich-plasma (PRP) has been accelerated to investigate early recovery from various musculoskeletal conditions. It involves the promotion of tissue damage repair through the action of multiple growth factors at physiological concentrations. The composition of PRP differs based on many factors, which may include age and gender. Therefore, we analyzed correlations between age, gender, and platelet counts in PRP with growth factors in Japanese subjects.

METHOD: Peripheral blood was drawn from 39 healthy volunteers between 20 and 49 years of age (age, mean ± standard deviation = 33 ± 8.7 years; gender ratio, male:female = 19:20; BMI, mean ± standard deviation = 22 ± 4.0) and prepared through centrifugation (volume, 6 mL per sample). After being activated with CaCl

RESULTS: Age was negatively correlated with platelet-derived growth factor-BB and insulin-like growth factor-1 (r = - 0.32, - 0.39), and gender had no influence on growth factors. Platelet counts in PRP positively correlated with platelet-derived growth factor-BB, transforming growth factor-β1, epidermal growth factor, and hepatocyte growth factor (r = 0.39, 0.75, 0.71, and 0.48, respectively).

CONCLUSIONS: This clinical study shows a significant variation of PRP among individual patients and that this variation is influenced by the age and the platelet counts of the subjects. Our data demonstrate that patient characteristics account for the differences in PRP physiological activity.

Keywords: Age; Gender; Growth factor; Platelet count; Platelet-rich plasma

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