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Parke JL, Linderman RG, Osterbauer NK, et al. Detection of Phytophthora ramorum Blight in Oregon Nurseries and Completion of Koch's Postulates on Pieris, Rhododendron, Viburnum, and Camellia. Plant Dis. 2004;88(1):87doi: 10.1094/PDIS.2004.88.1.87A.
Parke, J. L., Linderman, R. G., Osterbauer, N. K., & Griesbach, J. A. (2004). Detection of Phytophthora ramorum Blight in Oregon Nurseries and Completion of Koch's Postulates on Pieris, Rhododendron, Viburnum, and Camellia. Plant disease, 88(1), 87. https://doi.org/10.1094/PDIS.2004.88.1.87A
Parke, J L, et al. "Detection of Phytophthora ramorum Blight in Oregon Nurseries and Completion of Koch's Postulates on Pieris, Rhododendron, Viburnum, and Camellia." Plant disease vol. 88,1 (2004): 87. doi: https://doi.org/10.1094/PDIS.2004.88.1.87A
Parke JL, Linderman RG, Osterbauer NK, Griesbach JA. Detection of Phytophthora ramorum Blight in Oregon Nurseries and Completion of Koch's Postulates on Pieris, Rhododendron, Viburnum, and Camellia. Plant Dis. 2004 Jan;88(1):87. doi: 10.1094/PDIS.2004.88.1.87A. PMID: 30812475.
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Plant Dis. 2004 Jan;88(1):87. doi: 10.1094/PDIS.2004.88.1.87A.

Detection of Phytophthora ramorum Blight in Oregon Nurseries and Completion of Koch's Postulates on Pieris, Rhododendron, Viburnum, and Camellia.

Plant disease

J L Parke, R G Linderman, N K Osterbauer, J A Griesbach

Affiliations

  1. Oregon State University, Corvallis.
  2. USDA-ARS, Corvallis, OR.
  3. Oregon Department of Agriculture, Salem.

PMID: 30812475 DOI: 10.1094/PDIS.2004.88.1.87A

Abstract

Phytophthora ramorum, the cause of sudden oak death in California and Oregon coastal forests and ramorum blight in European nurseries and landscapes (1), was detected in six Oregon nurseries in Jackson, Clackamas, and Washington counties from May to June 2003. The pathogen was isolated from: Viburnum bodnantense 'Dawn', V. plicatum var. tomentosum 'Mariesii', Pieris japonica × formosa 'Forest Flame', P. japonica 'Variegata' and 'Flaming Silver', P. floribunda × japonica 'Brouwer's Beauty', Camellia sasanqua 'Bonanza' and other cultivars, C. japonica, and Rhododendron × 'Unique'. Samples of symptomatic tissues were plated on a Phytophthora-selective medium (PARP) and tested by polymerase chain reaction (PCR) (3). All samples positive for P. ramorum with PCR yielded P. ramorum isolates in culture. The isolates have the European genotype, mating type A1, except for the Camellia spp. isolates, which have the North American genotype, mating type A2 (2). Isolates are deposited in the American Type Culture Collection. Koch's postulates for this pathogen have been completed on V. bodnantense and C. japonica (1). To confirm pathogenicity on the new hosts, isolates from V. plicatum var. tomentosum 'Mariesii', Pieris × 'Forest Flame', Pieris × 'Brouwer's Beauty', and P. japonica 'Variegata' and 'Flaming Silver' were used to inoculate healthy plants of the same cultivars. For isolates from Rhododendron × 'Unique' and C. sasanqua 'Bonanza', pathogenicity was tested on Rhododendron × 'Nova Zembla' and C. sasanqua 'Sutsugekka' and 'Kanjiro'. Three to five plants of each cultivar were inoculated and three to five were noninoculated. Zoospore inoculum was prepared on dilute V8 agar for one isolate from each host. Foliage of plants growing in 10-cm pots was dipped for 5 sec in a zoospore suspension (3 × 10

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