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Plant Dis. 2000 Feb;84(2):143-147. doi: 10.1094/PDIS.2000.84.2.143.

Detection and Partial Characterization of Tenuiviruses from Black Spruce.

Plant disease

J D Castello, S O Rogers, G D Bachand, R C Fillhart, J S Murray, K Weidemann, M Bachand, M A Almond

Affiliations

  1. Professor.
  2. Associate Professor.
  3. Former Graduate Research Assistant.
  4. former Undergraduate Research Assistant, State University of New York, College of Environmental Science and Forestry, 1 Forestry Drive, Syracuse 13210-2788.

PMID: 30841304 DOI: 10.1094/PDIS.2000.84.2.143

Abstract

Filamentous viral ribonucleoproteins (RNPs) 12 to 16 nm in diameter and 100 to 1,260 nm in length, and characteristic of the genus Tenuivirus, were detected by transmission electron microscopy in purified extracts of needles collected from two mature, asymptomatic black spruce (Picea mariana) trees in New York, but not in extracts of needles from nursery seedlings. Purified RNPs from one tree had a buoyant density in CsCl = 1.39 g/cm

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