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Zhou J, Zhou Z, Ji P, et al. Effect of fecal microbiota transplantation on experimental colitis in mice. Exp Ther Med. 2019;17(4):2581-2586doi: 10.3892/etm.2019.7263.
Zhou, J., Zhou, Z., Ji, P., Ma, M., Guo, J., & Jiang, S. (2019). Effect of fecal microbiota transplantation on experimental colitis in mice. Experimental and therapeutic medicine, 17(4), 2581-2586. https://doi.org/10.3892/etm.2019.7263
Zhou, Julan, et al. "Effect of fecal microbiota transplantation on experimental colitis in mice." Experimental and therapeutic medicine vol. 17,4 (2019): 2581-2586. doi: https://doi.org/10.3892/etm.2019.7263
Zhou J, Zhou Z, Ji P, Ma M, Guo J, Jiang S. Effect of fecal microbiota transplantation on experimental colitis in mice. Exp Ther Med. 2019 Apr;17(4):2581-2586. doi: 10.3892/etm.2019.7263. Epub 2019 Feb 13. PMID: 30906449; PMCID: PMC6425147.
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Exp Ther Med. 2019 Apr;17(4):2581-2586. doi: 10.3892/etm.2019.7263. Epub 2019 Feb 13.

Effect of fecal microbiota transplantation on experimental colitis in mice.

Experimental and therapeutic medicine

Julan Zhou, Zhongyin Zhou, Panpan Ji, Min Ma, Jinkun Guo, Shujuan Jiang

Affiliations

  1. Department of Gastroenterology, Renmin Hospital of Wuhan University, Hubei Key Laboratory of Digestive System Disease, Wuhan, Hubei 430060, P.R. China.
  2. Department of Gastroenterology, Zhengzhou Third People's Hospital, Zhengzhou, Henan 450000, P.R. China.

PMID: 30906449 PMCID: PMC6425147 DOI: 10.3892/etm.2019.7263

Abstract

The aim of the present study was to investigate the effect of fecal microbiota transplantation (FMT) on the acute inflammatory response in a murine model of dextran sulfate sodium (DSS)-induced colitis, and to delineate the putative underlying mechanism(s). Mice were divided into four groups, namely the normal control, DSS, 5-aminosalicylic acid (5-ASA) and FMT group. Mice in the DSS, 5-ASA and FMT groups were orally administered 3% DSS (w/v) solution for 7 days to induce colitis. On days 1, 3, 5 and 7, mice in the DSS, 5-ASA and FMT groups were respectively administered 0.5% carboxymethylcellulose sodium, 5-ASA suspension and fecal suspension by enema. The disease activity index of each mouse was calculated on a daily basis. All mice were sacrificed on day 8, and the length of their colons was measured. Myeloperoxidase (MPO) activity, and the levels of tumor necrosis factor α (TNF-α), interleukin (IL)-1β and IL-10 in the colon tissues of each group were also measured. Compared with that in the DSS group, FMT ameliorated the severity of inflammation due to ulcerative colitis in mice, which was accompanied by a significantly decreased MPO activity, reduced levels of TNF-α and IL-1β, and an increased level of IL-10 in colon tissue (all P<0.05). Taken together, these results demonstrated that FMT exerted a therapeutic effect on experimental colitis in mice, and the associated mechanism is likely to involve the remodeling of the intestinal flora and regulation of intestinal T-cell immunity homeostasis.

Keywords: dysbacteriosis; fecal microbiota transplantation; immunity homeostasis; intestinal symbiotic flora; ulcerative colitis

References

  1. Clin Exp Immunol. 2000 Apr;120(1):51-8 - PubMed
  2. Nature. 2008 May 29;453(7195):620-5 - PubMed
  3. Biomaterials. 2008 Dec;29(34):4554-60 - PubMed
  4. Environ Microbiol. 2009 Jun;11(6):1562-71 - PubMed
  5. Nature. 2009 Oct 29;461(7268):1282-6 - PubMed
  6. Genome Res. 2010 Oct;20(10):1411-9 - PubMed
  7. Science. 2011 Jan 21;331(6015):337-41 - PubMed
  8. Nature. 2011 Jun 15;474(7351):298-306 - PubMed
  9. Am J Gastroenterol. 2012 May;107(5):761-7 - PubMed
  10. Genome Biol. 2012 Apr 16;13(9):R79 - PubMed
  11. Immunol Rev. 2013 Mar;252(1):164-82 - PubMed
  12. Science. 2013 Aug 2;341(6145):569-73 - PubMed
  13. Transl Res. 2016 Oct;176:38-68 - PubMed
  14. Transl Res. 2017 Jan;179:38-48 - PubMed
  15. Biotechnol Adv. 2016 Nov 15;34(7):1210-1224 - PubMed
  16. Expert Rev Gastroenterol Hepatol. 2018 May;12(5):439-445 - PubMed
  17. PLoS One. 2018 Jul 31;13(7):e0201356 - PubMed
  18. Lab Invest. 1993 Aug;69(2):238-49 - PubMed

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