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Rudakova SA, Rudakov NV, Petrova YA, et al. [The molecular biological methods of express-detection of pathogens in ticks taken way from patients as a basis of preventive therapy of tick transmissible infections.]. Klin Lab Diagn. 2017;62(10):615-618doi: 10.18821/0869-2084-2017-62-10-615-618.
Rudakova, S. A., Rudakov, N. V., Petrova, Y. A., Berezkina, G. V., Okolelova, N. A., & Kolomeetz, A. N. (2017). [The molecular biological methods of express-detection of pathogens in ticks taken way from patients as a basis of preventive therapy of tick transmissible infections.]. Klinicheskaia laboratornaia diagnostika, 62(10), 615-618. https://doi.org/10.18821/0869-2084-2017-62-10-615-618
Rudakova, S A, et al. "[The molecular biological methods of express-detection of pathogens in ticks taken way from patients as a basis of preventive therapy of tick transmissible infections.]." Klinicheskaia laboratornaia diagnostika vol. 62,10 (2017): 615-618. doi: https://doi.org/10.18821/0869-2084-2017-62-10-615-618
Rudakova SA, Rudakov NV, Petrova YA, Berezkina GV, Okolelova NA, Kolomeetz AN. [The molecular biological methods of express-detection of pathogens in ticks taken way from patients as a basis of preventive therapy of tick transmissible infections.]. Klin Lab Diagn. 2017;62(10):615-618. doi: 10.18821/0869-2084-2017-62-10-615-618. Russian. PMID: 30821943.
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Klin Lab Diagn. 2017;62(10):615-618. doi: 10.18821/0869-2084-2017-62-10-615-618.

[The molecular biological methods of express-detection of pathogens in ticks taken way from patients as a basis of preventive therapy of tick transmissible infections.].

Klinicheskaia laboratornaia diagnostika

[Article in Russian]
S A Rudakova, N V Rudakov, Yu A Petrova, G V Berezkina, N A Okolelova, A N Kolomeetz

Affiliations

  1. The Omskii research institute of feral nidal infections of the Rospotrebnadzor, 644080, Omsk, Russia.
  2. The Omskii state medical university of Minzdrav of Russia, 644050, Omsk, Russia.

PMID: 30821943 DOI: 10.18821/0869-2084-2017-62-10-615-618

Abstract

The complex molecular biologic study of ticks, took off from people in the Omskii oblast during Spring-Summer periods in 2014-2016 established their infection with agents of tick's transmissive infections and testifies a significant risk of infection of population. The analysis of ticks I. persulcatus established that rate of detection of DNA of borrelia made up to 59,5%, rickettsia - 30.6%, anaplasmae and erlichiae - 5.9%. The analysis of ticks D. reticulatus by polymerase chain reaction detected DNA of rickettsia in 20.5%, borrelia - in 6.6%, RNA of tick-borne encephalitis virus - in 3.6%. No DNA of anaplasmae and erlichiae was detected. During sequencing of tests positive to rickettsia the most often Rickettsia raoultii was identified. The approaches to expressdiagnostic and preventive therapy of tick-born transmissive infections were substantiated with consideration for mixt-infection of agents and pathogenic characteristics of developing nosologies. The algorithms of laboratory analysis regulated by the Sanitary Regulations 3.1.33-10-15 "Prevention of infections transmitted by ticks" require a broader implementation.

Keywords: express-diagnostic; preventive therapy; rickettsia and ricketsiosis; ticks' transmissive infections

Conflict of interest statement

The authors declare no conflict of interest.

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