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Sokolov AV, Kostina DA, Marinchev SS, et al. [The development of express-test for semiquantitative detection of thiocyanate ions in saliva.]. Klin Lab Diagn. 2017;62(12):730-734doi: 10.18821/0869-2084-2017-62-12-730-734.
Sokolov, A. V., Kostina, D. A., Marinchev, S. S., Chaplygin, S. S., & Kolsanov, A. V. (2017). [The development of express-test for semiquantitative detection of thiocyanate ions in saliva.]. Klinicheskaia laboratornaia diagnostika, 62(12), 730-734. https://doi.org/10.18821/0869-2084-2017-62-12-730-734
Sokolov, A V, et al. "[The development of express-test for semiquantitative detection of thiocyanate ions in saliva.]." Klinicheskaia laboratornaia diagnostika vol. 62,12 (2017): 730-734. doi: https://doi.org/10.18821/0869-2084-2017-62-12-730-734
Sokolov AV, Kostina DA, Marinchev SS, Chaplygin SS, Kolsanov AV. [The development of express-test for semiquantitative detection of thiocyanate ions in saliva.]. Klin Lab Diagn. 2017;62(12):730-734. doi: 10.18821/0869-2084-2017-62-12-730-734. Russian. PMID: 30856304.
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Klin Lab Diagn. 2017;62(12):730-734. doi: 10.18821/0869-2084-2017-62-12-730-734.

[The development of express-test for semiquantitative detection of thiocyanate ions in saliva.].

Klinicheskaia laboratornaia diagnostika

[Article in Russian]
A V Sokolov, D A Kostina, S S Marinchev, S S Chaplygin, A V Kolsanov

Affiliations

  1. The Federal state budget educational institution of higher education "The Samara state medical university" of Minzdrav of Russia, 443099, Samara, Russia.

PMID: 30856304 DOI: 10.18821/0869-2084-2017-62-12-730-734

Abstract

The article presents the results of approbation of the express test-system for semi-quantitative detection of content of thiocyanate ions in saliva as markers of tobacco smoking. The device corresponds to hollow tube with sensory element inside functioning on the principle of "dry chemistry". At saliva intake, the analyzing component (thiocyanate ion) interacts with reagents of sensory element with formation of colored thiocyanate complex. The intensity of color of formed complex permits to judge about content of thiocyanates in saliva by comparison with standard scale. The concentration of of thiocyanate ions in oral fluid of 100 health people of both genders aged from 16 to 45 years was analyzed. The questionnaire survey of respondents was carried out to establish smoking and non-smoking contingents. The analysis established that 30% were active tobacco smokers. The everyday smokers made up to 50% out of them, non-regular smokers (several cigarettes per week or month) - 50%. The reliable relationship between intensity of smoking and concentration of thiocyanate ions in saliva is established. The level of thiocyanate ions in saliva is significantly higher (2.5 mmol/l) in the group of everyday smokers than in saliva of non-smokers of periodically using tobacco articles (0.3-0.5 mmol/l) The increased concentrations of thiocyanate ions in saliva (≥ 1,5 mmol/l) were established in 7% of nonsmokers and are possible related to consumtion of food containing glucosinolates. The test is efficient for detecting smokers using from 1 to 10 cigarettes per day. However, it is of no use in case of sporadic type of smoking.

Keywords: dry chemistry; express-analysis; saliva; smoking; thiocyanate ion

Conflict of interest statement

The authors declare no conflict of interest.

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