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Bradley PH, Gibney PA, Botstein D, et al. Minor Isozymes Tailor Yeast Metabolism to Carbon Availability. mSystems. 2019;4(1)doi: 10.1128/mSystems.00170-18.
Bradley, P. H., Gibney, P. A., Botstein, D., Troyanskaya, O. G., & Rabinowitz, J. D. (2019). Minor Isozymes Tailor Yeast Metabolism to Carbon Availability. mSystems, 4(1), . https://doi.org/10.1128/mSystems.00170-18
Bradley, Patrick H, et al. "Minor Isozymes Tailor Yeast Metabolism to Carbon Availability." mSystems vol. 4,1 (2019). doi: https://doi.org/10.1128/mSystems.00170-18
Bradley PH, Gibney PA, Botstein D, Troyanskaya OG, Rabinowitz JD. Minor Isozymes Tailor Yeast Metabolism to Carbon Availability. mSystems. 2019 Feb 26;4(1). doi: 10.1128/mSystems.00170-18. eCollection 2019. PMID: 30834327; PMCID: PMC6392091.
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mSystems. 2019 Feb 26;4(1). doi: 10.1128/mSystems.00170-18. eCollection 2019.

Minor Isozymes Tailor Yeast Metabolism to Carbon Availability.

mSystems

Patrick H Bradley, Patrick A Gibney, David Botstein, Olga G Troyanskaya, Joshua D Rabinowitz

Affiliations

  1. Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA.
  2. Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA.
  3. Department of Computer Science, Princeton University, Princeton, New Jersey, USA.
  4. Department of Chemistry, Princeton University, Princeton, New Jersey, USA.

PMID: 30834327 PMCID: PMC6392091 DOI: 10.1128/mSystems.00170-18

Abstract

Isozymes are enzymes that differ in sequence but catalyze the same chemical reactions. Despite their apparent redundancy, isozymes are often retained over evolutionary time, suggesting that they contribute to fitness. We developed an unsupervised computational method for identifying environmental conditions under which isozymes are likely to make fitness contributions. This method analyzes published gene expression data to find specific experimental perturbations that induce differential isozyme expression. In yeast, we found that isozymes are strongly enriched in the pathways of central carbon metabolism and that many isozyme pairs show anticorrelated expression during the respirofermentative shift. Building on these observations, we assigned function to two minor central carbon isozymes, aconitase 2 (

Keywords: cerevisiae; duplicates; genomics; isozymes; metabolomics; paralogs; systems biology; transcriptomics; yeast

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